Assessment of viability of wharton's jelly mesenchymal stem cells encapsulated in alginate scaffold by WST-8 assay kit

Q4 Biochemistry, Genetics and Molecular Biology Medical Journal of Cell Biology Pub Date : 2021-03-01 DOI:10.2478/acb-2021-0007

Zeinab Shafiei Seifabadi, Fatemeh Rezaei-Tazangi, Nastaran Azarbarz, D. Nejad, J. Mohammadiasl, Hanieh Darabi, Seyed Pezhmanlarki-Tork

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引用次数: 2

Abstract

Abstract Cell encapsulation utilizing biodegradable material has promising outcomes for tissue engineering. From a long time ago, alginate has been generally utilized for drug delivery, cell transplantation and as a scaffold in biomedical applications. The aim of this study was the comparison of cell viability in the presence of two polymerizing ions: Ba2+ and Ca2+ to improvement the quality of alginate scaffold. For this purpose, WJMSCs after three passage were encapsulated in alginate scaffold in the presence of Ba2+ and ca2+. Cell viability was evaluated by WST-8 assay kit after 24, 48 and 72 hours. The results showed that encapsulated cells in the presence of Ca2+ had more viability than Ba2+. It was also found that using the WST-8 assay kit is a convenient and fast method for evaluation the viability of cells. It can be claimed that Calcl2 polymerizing solution provides more favorable conditions for cell viability compared to Bacl2 solution. Running title: Assessing the viability of stem cells by WST-8 assay kit

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WST-8试剂盒测定海藻酸盐支架包封沃顿氏果冻间充质干细胞的生存能力

利用生物可降解材料对细胞进行包埋在组织工程中具有广阔的应用前景。长期以来，藻酸盐一直被广泛用于药物传递、细胞移植和生物医学中的支架。本研究的目的是比较在Ba2+和Ca2+两种聚合离子存在下的细胞活力，以提高海藻酸盐支架的质量。为此，在Ba2+和ca2+存在下，将三代后的WJMSCs包被在海藻酸盐支架中。24h、48h和72h后，采用WST-8检测试剂盒检测细胞活力。结果表明，Ca2+存在时囊化细胞比Ba2+具有更高的生存能力。同时发现WST-8检测试剂盒是一种方便、快速的细胞活力评价方法。可以认为，与Bacl2溶液相比，Calcl2聚合溶液提供了更有利的细胞生存条件。标题:用WST-8检测试剂盒评估干细胞活力

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Medical Journal of Cell Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

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