Evaluation of mPEG-PLA nanoparticles as vaccine delivery system for modified protective antigen of Bacillus anthracis

IF 1.4 Q4 NANOSCIENCE & NANOTECHNOLOGY Nanomedicine Journal Pub Date : 2021-10-01 DOI:10.22038/NMJ.2021.59843.1616
Seyed masih e'temad aubi, H. Honari, H. Bagheri, R. Ghasemi, M. Noofeli, S. M. Aghaie
{"title":"Evaluation of mPEG-PLA nanoparticles as vaccine delivery system for modified protective antigen of Bacillus anthracis","authors":"Seyed masih e'temad aubi, H. Honari, H. Bagheri, R. Ghasemi, M. Noofeli, S. M. Aghaie","doi":"10.22038/NMJ.2021.59843.1616","DOIUrl":null,"url":null,"abstract":"Objective(s) Bacillus anthracis is the cause of the fatal anthrax. Available anthrax vaccines have low stability and require multiple injections in order to be effective. Poly lactic acid (PLA) has been approved as a biodegradable and biocompatible polymer for drug and vaccine delivery applications. The purpose of this study is to evaluate the antibody titer against the protective antigen recombinant protein (PA63) encapsulated by the mPEG-PLA double-block copolymers and to compare with the non-encapsulated PA63.Materials and Methods To attain this purpose, to start, the desired protein was purified and confirmed and then PA63 was encapsulated with mPEG-PLA double-block copolymers using a water- oil- water solvent evaporation method. Produced nanoparticles was characterized in terms of morphological specifications using scanning electron microscopy, size and polydispersity index using dynamic light scattering and zeta potential using a zeta seizer. The synthesized nanoparticle antigenic content and also its antigen release profile was measured. In the following, the nanoparticles containing antigens (PA63-NPs), blank nanoparticles (mPEG-PLA- NPs), PA63 and adjuvant control were injected subcutaneously to mice and the IgG polyclonal antibody titr was measured by indirect ELISA. Finally to evaluate biocompatibility and toxicity, synthesized nanoparticles were investigated  by cell culture testing.Results  The results of this study showed that the synthesized nanoparticles are of good quality. ELISA results showed that antibody production titr in mice receiving PA63-NPs was higher than those receiving the PA63 (P<0.05). Cell culture results revealed that the synthesized nanoparticles have no toxicity.Conclusion The findings of the study indicated that the obtained nano vaccine formulations had a higher ability than non-encapsulated recombinant proteins to stimulate the immune system of animal, and that PLA could be used as an appropriate carrier for an effective, stable, safe and biodegradable engineered recombinant vaccine against anthrax.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanomedicine Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22038/NMJ.2021.59843.1616","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"NANOSCIENCE & NANOTECHNOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Objective(s) Bacillus anthracis is the cause of the fatal anthrax. Available anthrax vaccines have low stability and require multiple injections in order to be effective. Poly lactic acid (PLA) has been approved as a biodegradable and biocompatible polymer for drug and vaccine delivery applications. The purpose of this study is to evaluate the antibody titer against the protective antigen recombinant protein (PA63) encapsulated by the mPEG-PLA double-block copolymers and to compare with the non-encapsulated PA63.Materials and Methods To attain this purpose, to start, the desired protein was purified and confirmed and then PA63 was encapsulated with mPEG-PLA double-block copolymers using a water- oil- water solvent evaporation method. Produced nanoparticles was characterized in terms of morphological specifications using scanning electron microscopy, size and polydispersity index using dynamic light scattering and zeta potential using a zeta seizer. The synthesized nanoparticle antigenic content and also its antigen release profile was measured. In the following, the nanoparticles containing antigens (PA63-NPs), blank nanoparticles (mPEG-PLA- NPs), PA63 and adjuvant control were injected subcutaneously to mice and the IgG polyclonal antibody titr was measured by indirect ELISA. Finally to evaluate biocompatibility and toxicity, synthesized nanoparticles were investigated  by cell culture testing.Results  The results of this study showed that the synthesized nanoparticles are of good quality. ELISA results showed that antibody production titr in mice receiving PA63-NPs was higher than those receiving the PA63 (P<0.05). Cell culture results revealed that the synthesized nanoparticles have no toxicity.Conclusion The findings of the study indicated that the obtained nano vaccine formulations had a higher ability than non-encapsulated recombinant proteins to stimulate the immune system of animal, and that PLA could be used as an appropriate carrier for an effective, stable, safe and biodegradable engineered recombinant vaccine against anthrax.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
mPEG-PLA纳米颗粒作为炭疽芽孢杆菌修饰保护性抗原疫苗递送系统的评价
目的炭疽杆菌是造成致命炭疽病的原因。现有的炭疽疫苗稳定性低,需要多次注射才能有效。聚乳酸(PLA)已被批准为一种可生物降解和生物相容性聚合物,用于药物和疫苗递送应用。本研究的目的是评估由mPEG-PLA双嵌段共聚物包封的保护性抗原重组蛋白(PA63)的抗体滴度,并与未包封的PA63进行比较,纯化并确认所需的蛋白质,然后使用水-油-水溶剂蒸发法用mPEG-PLA双嵌段共聚物包封PA63。使用扫描电子显微镜对所制备的纳米颗粒的形态规格、使用动态光散射的尺寸和多分散指数以及使用ζ-seizer的ζ电势进行表征。测量了合成的纳米颗粒抗原含量及其抗原释放谱。在下文中,将含有抗原的纳米颗粒(PA63NP)、空白纳米颗粒(mPEG-PLA-NPs)、PA63和佐剂对照皮下注射到小鼠,并通过间接ELISA测量IgG多克隆抗体滴度。最后,通过细胞培养试验对合成的纳米颗粒进行了研究,以评价其生物相容性和毒性。结果本研究结果表明,合成的纳米颗粒具有良好的质量。ELISA结果显示,PA63纳米粒子对小鼠的抗体产生滴度高于PA63纳米粒子(P<0.05),细胞培养结果表明,合成的纳米粒子无毒性。结论所制备的纳米疫苗制剂比未包封的重组蛋白具有更高的刺激动物免疫系统的能力,PLA可作为有效、稳定、安全、可生物降解的炭疽工程重组疫苗的合适载体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Nanomedicine Journal
Nanomedicine Journal NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
3.40
自引率
0.00%
发文量
0
审稿时长
12 weeks
期刊最新文献
Nano aptasensors for detection of streptomycin: A review Synthesis of silver nanoparticles by Galega officinalis and its hypoglycemic effects in type 1 diabetic rats Evaluation of mPEG-PLA nanoparticles as vaccine delivery system for modified protective antigen of Bacillus anthracis Synthesis and evaluation of SPION@CMD@Ser-LTVSPWY peptide as a targeted probe for detection of HER2+ cancer cells in MRI Synthesis of L-DOPA conjugated doxorubicin-polyethylenimine nanocarrier and evaluation of its cytotoxicity on A375 and HepG2 cell lines
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1