Pub Date : 2021-11-15DOI: 10.22038/NMJ.2021.60108.1622
Asefeh Dahmardeh Ghalehno, M. Saeedi, S. R. Bazaz, P. Asadi, M. Warkiani, Rezvan Yazdian-Robati
This review provides a literature update of the progress in optical and electrochemical aptasensors for the detection of streptomycin in human sera and animal-derived foods. The uncontrolled use of antibiotics and rising resistance to them, has created a global problem. Therefore, the detection and quantitation of antibiotics, i.e., streptomycin by robust, easy, and sensitive methods is in great demand. Among different strategies, new analytical methods for the efficient detection and quantitative determination of streptomycin have been developed. Aptasensors or aptamer-based biosensors have attracted more attention due to their unique recognition, simple fabrication, and significant selectivity, sensitivity, and specificity. Advantages of aptasensors will be highlighted in this review, with emphasis on methodological technique and specific properties of aptasensors developed for STR determination. In this review paper, we will focus on the recent development of aptasensors for streptomycin detection, considering the papers summarized in the data bases scopus and google scholar covering the period of time from 2013 till 2021.
{"title":"Nano aptasensors for detection of streptomycin: A review","authors":"Asefeh Dahmardeh Ghalehno, M. Saeedi, S. R. Bazaz, P. Asadi, M. Warkiani, Rezvan Yazdian-Robati","doi":"10.22038/NMJ.2021.60108.1622","DOIUrl":"https://doi.org/10.22038/NMJ.2021.60108.1622","url":null,"abstract":"This review provides a literature update of the progress in optical and electrochemical aptasensors for the detection of streptomycin in human sera and animal-derived foods. The uncontrolled use of antibiotics and rising resistance to them, has created a global problem. Therefore, the detection and quantitation of antibiotics, i.e., streptomycin by robust, easy, and sensitive methods is in great demand. Among different strategies, new analytical methods for the efficient detection and quantitative determination of streptomycin have been developed. Aptasensors or aptamer-based biosensors have attracted more attention due to their unique recognition, simple fabrication, and significant selectivity, sensitivity, and specificity. Advantages of aptasensors will be highlighted in this review, with emphasis on methodological technique and specific properties of aptasensors developed for STR determination. In this review paper, we will focus on the recent development of aptasensors for streptomycin detection, considering the papers summarized in the data bases scopus and google scholar covering the period of time from 2013 till 2021.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48383150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.22038/NMJ.2021.59843.1616
Seyed masih e'temad aubi, H. Honari, H. Bagheri, R. Ghasemi, M. Noofeli, S. M. Aghaie
Objective(s) Bacillus anthracis is the cause of the fatal anthrax. Available anthrax vaccines have low stability and require multiple injections in order to be effective. Poly lactic acid (PLA) has been approved as a biodegradable and biocompatible polymer for drug and vaccine delivery applications. The purpose of this study is to evaluate the antibody titer against the protective antigen recombinant protein (PA63) encapsulated by the mPEG-PLA double-block copolymers and to compare with the non-encapsulated PA63.Materials and Methods To attain this purpose, to start, the desired protein was purified and confirmed and then PA63 was encapsulated with mPEG-PLA double-block copolymers using a water- oil- water solvent evaporation method. Produced nanoparticles was characterized in terms of morphological specifications using scanning electron microscopy, size and polydispersity index using dynamic light scattering and zeta potential using a zeta seizer. The synthesized nanoparticle antigenic content and also its antigen release profile was measured. In the following, the nanoparticles containing antigens (PA63-NPs), blank nanoparticles (mPEG-PLA- NPs), PA63 and adjuvant control were injected subcutaneously to mice and the IgG polyclonal antibody titr was measured by indirect ELISA. Finally to evaluate biocompatibility and toxicity, synthesized nanoparticles were investigated by cell culture testing.Results The results of this study showed that the synthesized nanoparticles are of good quality. ELISA results showed that antibody production titr in mice receiving PA63-NPs was higher than those receiving the PA63 (P<0.05). Cell culture results revealed that the synthesized nanoparticles have no toxicity.Conclusion The findings of the study indicated that the obtained nano vaccine formulations had a higher ability than non-encapsulated recombinant proteins to stimulate the immune system of animal, and that PLA could be used as an appropriate carrier for an effective, stable, safe and biodegradable engineered recombinant vaccine against anthrax.
{"title":"Evaluation of mPEG-PLA nanoparticles as vaccine delivery system for modified protective antigen of Bacillus anthracis","authors":"Seyed masih e'temad aubi, H. Honari, H. Bagheri, R. Ghasemi, M. Noofeli, S. M. Aghaie","doi":"10.22038/NMJ.2021.59843.1616","DOIUrl":"https://doi.org/10.22038/NMJ.2021.59843.1616","url":null,"abstract":"Objective(s) Bacillus anthracis is the cause of the fatal anthrax. Available anthrax vaccines have low stability and require multiple injections in order to be effective. Poly lactic acid (PLA) has been approved as a biodegradable and biocompatible polymer for drug and vaccine delivery applications. The purpose of this study is to evaluate the antibody titer against the protective antigen recombinant protein (PA63) encapsulated by the mPEG-PLA double-block copolymers and to compare with the non-encapsulated PA63.Materials and Methods To attain this purpose, to start, the desired protein was purified and confirmed and then PA63 was encapsulated with mPEG-PLA double-block copolymers using a water- oil- water solvent evaporation method. Produced nanoparticles was characterized in terms of morphological specifications using scanning electron microscopy, size and polydispersity index using dynamic light scattering and zeta potential using a zeta seizer. The synthesized nanoparticle antigenic content and also its antigen release profile was measured. In the following, the nanoparticles containing antigens (PA63-NPs), blank nanoparticles (mPEG-PLA- NPs), PA63 and adjuvant control were injected subcutaneously to mice and the IgG polyclonal antibody titr was measured by indirect ELISA. Finally to evaluate biocompatibility and toxicity, synthesized nanoparticles were investigated by cell culture testing.Results The results of this study showed that the synthesized nanoparticles are of good quality. ELISA results showed that antibody production titr in mice receiving PA63-NPs was higher than those receiving the PA63 (P<0.05). Cell culture results revealed that the synthesized nanoparticles have no toxicity.Conclusion The findings of the study indicated that the obtained nano vaccine formulations had a higher ability than non-encapsulated recombinant proteins to stimulate the immune system of animal, and that PLA could be used as an appropriate carrier for an effective, stable, safe and biodegradable engineered recombinant vaccine against anthrax.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42459550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.22038/NMJ.2021.60221.1623
Sanaz Abbaszadegan, H. Hosseinzadeh, S. Alavizadeh, Marziyeh Moghri, A. Abbasi, M. Jaafari
Objective(s): Saffron, the dehydrated stigma of the Crocus sativus L. flower, has been reputed as an effective anticancer and chemopreventive agent in cancer therapy. This study aimed to design PEGylated nanoliposomes containing crude extract of saffron for the treatment of cancer.Materials and Methods: Various PEGylated nanoliposomes containing 25 mg/ml aqueous extract of saffron were prepared using the thin lipid film method. The characterization of liposomes was indicated by their size, in vitro cytotoxicity, and in vivo therapeutic efficacy against C26 tumor-bearing mice. Results: By increasing cholesterol levels, the IC50 values of the formulations increased. Liposome characterization illustrated the properties of formulation of choice, as follows: Z-average size: 73.7 ± 1.3 nm; PDI: 0.103 ± 0.035; zeta potential: -20.8 mV ± 3.7; % encapsulation: 91 ± 0.059, % release after 168 hours in 30% FBS: 16.26 ± 0.01.Conclusion: Treating tumor-bearing mice with the selected saffron liposomes indicated that, for the first time, the i.v. injection of nano-liposomal saffron at a dose of 300 mg/kg significantly increased the anti-tumor property compared to the negative control group, while no significant difference was observed compared with aqueous extract of saffron. Hence, to achieve an optimal formulation for human use, the formulation merits further study.
{"title":"Anti-tumor activity of nanoliposomes containing crude extract of saffron in mice bearing C26 colon carcinoma","authors":"Sanaz Abbaszadegan, H. Hosseinzadeh, S. Alavizadeh, Marziyeh Moghri, A. Abbasi, M. Jaafari","doi":"10.22038/NMJ.2021.60221.1623","DOIUrl":"https://doi.org/10.22038/NMJ.2021.60221.1623","url":null,"abstract":"Objective(s): Saffron, the dehydrated stigma of the Crocus sativus L. flower, has been reputed as an effective anticancer and chemopreventive agent in cancer therapy. This study aimed to design PEGylated nanoliposomes containing crude extract of saffron for the treatment of cancer.Materials and Methods: Various PEGylated nanoliposomes containing 25 mg/ml aqueous extract of saffron were prepared using the thin lipid film method. The characterization of liposomes was indicated by their size, in vitro cytotoxicity, and in vivo therapeutic efficacy against C26 tumor-bearing mice. Results: By increasing cholesterol levels, the IC50 values of the formulations increased. Liposome characterization illustrated the properties of formulation of choice, as follows: Z-average size: 73.7 ± 1.3 nm; PDI: 0.103 ± 0.035; zeta potential: -20.8 mV ± 3.7; % encapsulation: 91 ± 0.059, % release after 168 hours in 30% FBS: 16.26 ± 0.01.Conclusion: Treating tumor-bearing mice with the selected saffron liposomes indicated that, for the first time, the i.v. injection of nano-liposomal saffron at a dose of 300 mg/kg significantly increased the anti-tumor property compared to the negative control group, while no significant difference was observed compared with aqueous extract of saffron. Hence, to achieve an optimal formulation for human use, the formulation merits further study.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46590622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.22038/NMJ.2021.58980.1607
Noorin Yousefiyeh, Abolfazl Arab, Elnaz Salehian, R. Alikhani, Shabnam Samimi, A. Assadi, M. Ardestani
Objective(s): Cancer is known as one of the most life-threatening diseases in the world. Early diagnosis of cancer may significantly increase the chance of effective treatment. In the recent years, the importance of medical imaging usage has been increased to identify cancer’s nature and pattern of growth in order to provide the most advantageous treatment approaches for cancer tumors. Magnetic resonance imaging is an efficient non-invasive tool for early diagnosis of cancer which provides clear scans of various tissues without radiation. Contrast Agents such as Gadoterate Meglumine enhance contrast MR imaging and provide imaging from inside the cells without entering them.Materials and Methods: In this study, Gadoterate Meglumine nano-sized anionic linear globular dendrimer second generation was first synthesized and then qualitative and quantitative methods were carried out to ensure the proper synthesis and to assess the toxicity of the compound. Once the non-toxicity of the chemical was ensured, in vivo MR imaging studies was performed to test the impact of the synthesized compound on the resolution of image. Results: The result obtained from this study demonstrated that the attachment of Gadolinium (III) to a nano dendrimer reduces its cytotoxicity and also improved resolution of image. In this research, Gadoterate Meglumine nano-sized anionic linear globular dendrimer second generation was effectively able to enter the cells while showing low cytotoxicity in the normal cells and moderate cytotoxicity on cancer cells. Conclusion: Therefore, ALGDG2-GM could be introduced as a novel, safe, effective and promising nano-sized theranostic contrast agent candidate.
{"title":"Gadoterate meglumine - anionic linear globular dendrimer second generation: A novel nano sized theranostic contrast agent","authors":"Noorin Yousefiyeh, Abolfazl Arab, Elnaz Salehian, R. Alikhani, Shabnam Samimi, A. Assadi, M. Ardestani","doi":"10.22038/NMJ.2021.58980.1607","DOIUrl":"https://doi.org/10.22038/NMJ.2021.58980.1607","url":null,"abstract":"Objective(s): Cancer is known as one of the most life-threatening diseases in the world. Early diagnosis of cancer may significantly increase the chance of effective treatment. In the recent years, the importance of medical imaging usage has been increased to identify cancer’s nature and pattern of growth in order to provide the most advantageous treatment approaches for cancer tumors. Magnetic resonance imaging is an efficient non-invasive tool for early diagnosis of cancer which provides clear scans of various tissues without radiation. Contrast Agents such as Gadoterate Meglumine enhance contrast MR imaging and provide imaging from inside the cells without entering them.Materials and Methods: In this study, Gadoterate Meglumine nano-sized anionic linear globular dendrimer second generation was first synthesized and then qualitative and quantitative methods were carried out to ensure the proper synthesis and to assess the toxicity of the compound. Once the non-toxicity of the chemical was ensured, in vivo MR imaging studies was performed to test the impact of the synthesized compound on the resolution of image. Results: The result obtained from this study demonstrated that the attachment of Gadolinium (III) to a nano dendrimer reduces its cytotoxicity and also improved resolution of image. In this research, Gadoterate Meglumine nano-sized anionic linear globular dendrimer second generation was effectively able to enter the cells while showing low cytotoxicity in the normal cells and moderate cytotoxicity on cancer cells. Conclusion: Therefore, ALGDG2-GM could be introduced as a novel, safe, effective and promising nano-sized theranostic contrast agent candidate.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49446007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.22038/NMJ.2021.59629.1614
Arash Papi, Rasoul Irajirad, Milad Yousefvand, A. Montazerabadi, Z. Mohammadi
Objective(s): Successful detection of tumors in the early stages can significantly increase a patient’s healing process and recovery speed. Conventional imaging techniques usually depend on the tissues’ anatomical structure. Epidermal growth factor receptor-2 (HER-2) is a transmembrane protein with an extracellular ligand-binding domain. HER2 plays an essential role in cell proliferation, differentiation, and survival, and its overexpression is associated with various cancers, especially breast and ovarian cancers. Access to its extracellular domain makes HER2 an ideal target for drug preparation and molecular imaging probes. In this study, a targeted magnetic nanoprobe for molecular imaging of HER2 positive cancers was synthesized, and also its potential as a T2-weighted targeted contrast agent was assessed.Materials and Methods: Superparamagnetic SPION nanoparticles were synthesized using the co-precipitation method in the presence of CMD and were labeled with SLTVSPWY peptide. The SPION@CMD@SLTVSPWY nanocomplex was characterized by TEM, DLS, XRD, AAS, FTIR, EDX, and VSM. The r1 and r2 relaxivities were then calculated using a 1.5 Tesla clinical magnetic field. The cytotoxicity of the nanocomplex was evaluated by MTT assay. Finally, the difference between uptake of targeted nanocomplexes and SPION by the human SKOV-3 cell line (HER2 +) was investigated.Results: The SPION@CMD NPs were synthesized with spherical shape and superparamagnetic behavior. Characterization results confirmed the formation of SPION@CMD@SLTVSPWY. r2 relaxivity and r2/r1 calculations resulted in suitable values of 313 mM-1s-1 and 8.05 for SPION@CMD@SLTVSPWY, respectively. Increased uptake of targeted nanocomplexed (SPION@CMD@SLTVSPWY) compared to non-targeted NPs (SPION@CMD) was very noticeable visually, and its numerical ratio was 3.51 at a concentration of 0.075 mM. Conclusion: The targeted synthesized nanocomplex in this study has great potential as a T2 weighted probe contrast agent in MR imaging owing to its appropriate high uptake in HER2 + cells.
{"title":"Synthesis and evaluation of SPION@CMD@Ser-LTVSPWY peptide as a targeted probe for detection of HER2+ cancer cells in MRI","authors":"Arash Papi, Rasoul Irajirad, Milad Yousefvand, A. Montazerabadi, Z. Mohammadi","doi":"10.22038/NMJ.2021.59629.1614","DOIUrl":"https://doi.org/10.22038/NMJ.2021.59629.1614","url":null,"abstract":"Objective(s): Successful detection of tumors in the early stages can significantly increase a patient’s healing process and recovery speed. Conventional imaging techniques usually depend on the tissues’ anatomical structure. Epidermal growth factor receptor-2 (HER-2) is a transmembrane protein with an extracellular ligand-binding domain. HER2 plays an essential role in cell proliferation, differentiation, and survival, and its overexpression is associated with various cancers, especially breast and ovarian cancers. Access to its extracellular domain makes HER2 an ideal target for drug preparation and molecular imaging probes. In this study, a targeted magnetic nanoprobe for molecular imaging of HER2 positive cancers was synthesized, and also its potential as a T2-weighted targeted contrast agent was assessed.Materials and Methods: Superparamagnetic SPION nanoparticles were synthesized using the co-precipitation method in the presence of CMD and were labeled with SLTVSPWY peptide. The SPION@CMD@SLTVSPWY nanocomplex was characterized by TEM, DLS, XRD, AAS, FTIR, EDX, and VSM. The r1 and r2 relaxivities were then calculated using a 1.5 Tesla clinical magnetic field. The cytotoxicity of the nanocomplex was evaluated by MTT assay. Finally, the difference between uptake of targeted nanocomplexes and SPION by the human SKOV-3 cell line (HER2 +) was investigated.Results: The SPION@CMD NPs were synthesized with spherical shape and superparamagnetic behavior. Characterization results confirmed the formation of SPION@CMD@SLTVSPWY. r2 relaxivity and r2/r1 calculations resulted in suitable values of 313 mM-1s-1 and 8.05 for SPION@CMD@SLTVSPWY, respectively. Increased uptake of targeted nanocomplexed (SPION@CMD@SLTVSPWY) compared to non-targeted NPs (SPION@CMD) was very noticeable visually, and its numerical ratio was 3.51 at a concentration of 0.075 mM. Conclusion: The targeted synthesized nanocomplex in this study has great potential as a T2 weighted probe contrast agent in MR imaging owing to its appropriate high uptake in HER2 + cells.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42717275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.22038/NMJ.2021.59391.1613
Fariba Azimi, F. Mahmoudi, Farzaneh Mahmoudi, M. Amini
Objective(s): Diabetes is related with the higher blood levels of liver enzymes and inflammatory factors. Galega officinalis is used as a medicinal plant for treatment of diabetes traditionally. In this work, silver nanoparticles (Ag-NPs) were synthesized with green method using Galega officinalis extract.Materials and Methods: The synthesized green Ag-NPs were characterized completely. Intact or diabetic rats receieved intraperitoneal injection of saline or 2/5mg/Kg green synthesized Ag-NPs. Mean serum levels of glucose, hepatic enzymes and hematological parameter were determined. Gene expression of tumor necrotic factor alpha (TNF-α) was done by real-time PCR. Results: Synthesis of green synthesized Ag-NPs was confirmed by FT-IR, XRD and UV-vis analyses. The FESEM and TEM images showed spherical Ag-NPs with size of 25 nm. The hypoglycemic influence of Ag-NPs using Galega officinalis extract is reported for the first time in this study. Blood concentration of liver enzymes, urea, glucose, white blood cells count and TNF-α mRNA levels in visceral adipose tissue significantly declined in diabetic rats receiving Ag-NPs.Conclusion: The synthesized Ag-NPs using Galega officinalis extract may improve complication of diabetes via preventing liver hepatocyte damage and reducing inflammatory factors.
{"title":"Synthesis of silver nanoparticles by Galega officinalis and its hypoglycemic effects in type 1 diabetic rats","authors":"Fariba Azimi, F. Mahmoudi, Farzaneh Mahmoudi, M. Amini","doi":"10.22038/NMJ.2021.59391.1613","DOIUrl":"https://doi.org/10.22038/NMJ.2021.59391.1613","url":null,"abstract":"Objective(s): Diabetes is related with the higher blood levels of liver enzymes and inflammatory factors. Galega officinalis is used as a medicinal plant for treatment of diabetes traditionally. In this work, silver nanoparticles (Ag-NPs) were synthesized with green method using Galega officinalis extract.Materials and Methods: The synthesized green Ag-NPs were characterized completely. Intact or diabetic rats receieved intraperitoneal injection of saline or 2/5mg/Kg green synthesized Ag-NPs. Mean serum levels of glucose, hepatic enzymes and hematological parameter were determined. Gene expression of tumor necrotic factor alpha (TNF-α) was done by real-time PCR. Results: Synthesis of green synthesized Ag-NPs was confirmed by FT-IR, XRD and UV-vis analyses. The FESEM and TEM images showed spherical Ag-NPs with size of 25 nm. The hypoglycemic influence of Ag-NPs using Galega officinalis extract is reported for the first time in this study. Blood concentration of liver enzymes, urea, glucose, white blood cells count and TNF-α mRNA levels in visceral adipose tissue significantly declined in diabetic rats receiving Ag-NPs.Conclusion: The synthesized Ag-NPs using Galega officinalis extract may improve complication of diabetes via preventing liver hepatocyte damage and reducing inflammatory factors.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42035427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.22038/NMJ.2021.59681.1615
Kimia Mansouri, F. Ahmadi, A. Dehshahri
Objective(s): Polyethylenimine (PEI) is one of the most-extensively investigated cationic polymers for gene and drug delivery. Recently, great attention has been directed to design of carriers for co-delivery of nucleic acids and small molecules. These delivery systems are able to overcome the limitations of gene or drug delivery alone. The aim of this study is to prepare a targeted nano-carrier for co-delivery of doxorubicin (Dox) and gene using polyethylenimine. Materials and Methods: In order to prepare the ligand-containing polymer conjugates, succinic anhydride was conjugated onto the hydroxyl group of Dox through an ester bond following the protection of Dox amines by Fmoc. Drug-polymer conjugates were then coupled with L-DOPA in order to prepare the targeted nanocarriers to the cells through Large Amino Acid Transporter-1 (LAT-1). The PEI derivatives were characterized using 1H-NMR. The toxicity of conjugated polymer, Dox and PEI was assessed on HepG2 and A375 cell lines with different expression level of LAT-1 transporters using MTT assay. Results: The chemical structure of PEI conjugate was confirmed by 1H-NMR. The cytotoxicity measurement demonstrated a cell line-dependent toxicity profile at the concentrations tested in this study. It was shown that there was no significant difference in cell-induced toxicity between conjugated polymer and its parent form in A375 cell line while the cytotoxicity of conjugated polymer was significantly lower than the parent PEI in HepG2 cells.Conclusion: These results provide promising evidence for further evaluation of PEI conjugate for co-delivery of drug and gene via LAT-1 transporters.
{"title":"Synthesis of L-DOPA conjugated doxorubicin-polyethylenimine nanocarrier and evaluation of its cytotoxicity on A375 and HepG2 cell lines","authors":"Kimia Mansouri, F. Ahmadi, A. Dehshahri","doi":"10.22038/NMJ.2021.59681.1615","DOIUrl":"https://doi.org/10.22038/NMJ.2021.59681.1615","url":null,"abstract":"Objective(s): Polyethylenimine (PEI) is one of the most-extensively investigated cationic polymers for gene and drug delivery. Recently, great attention has been directed to design of carriers for co-delivery of nucleic acids and small molecules. These delivery systems are able to overcome the limitations of gene or drug delivery alone. The aim of this study is to prepare a targeted nano-carrier for co-delivery of doxorubicin (Dox) and gene using polyethylenimine. Materials and Methods: In order to prepare the ligand-containing polymer conjugates, succinic anhydride was conjugated onto the hydroxyl group of Dox through an ester bond following the protection of Dox amines by Fmoc. Drug-polymer conjugates were then coupled with L-DOPA in order to prepare the targeted nanocarriers to the cells through Large Amino Acid Transporter-1 (LAT-1). The PEI derivatives were characterized using 1H-NMR. The toxicity of conjugated polymer, Dox and PEI was assessed on HepG2 and A375 cell lines with different expression level of LAT-1 transporters using MTT assay. Results: The chemical structure of PEI conjugate was confirmed by 1H-NMR. The cytotoxicity measurement demonstrated a cell line-dependent toxicity profile at the concentrations tested in this study. It was shown that there was no significant difference in cell-induced toxicity between conjugated polymer and its parent form in A375 cell line while the cytotoxicity of conjugated polymer was significantly lower than the parent PEI in HepG2 cells.Conclusion: These results provide promising evidence for further evaluation of PEI conjugate for co-delivery of drug and gene via LAT-1 transporters.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46497236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-18DOI: 10.22038/NMJ.2021.56425.1575
M. Mansouri, D. Shahbazi-Gahrouei
Application of nanoparticles have in the core of researchers attention for both imaging and therapy of cancers. This review article aimed to prepare an outline on recent applications of iodine nanoparticles (INPs) as theranostic agents in both diagnosis and therapies. Among various strategies are used in treatment of cancers, radiotherapy with radiopharmaceutical agents especially radioisotope of iodine displays satisfactory results for numerous types of cancers. In recent years, new investigations were done in order to develop the novel structure of INPs. These nanoprobes could act as efficient theranostic purposes. Iodine nanoparticles may be applied in nuclear medicine imaging and may be effective with mega voltage (MV) photons in cancer therapy, but this remains to be tested with different cancer cells. By using INPs, effective steps can be taken in the future in both diagnosis and treatment of cancers. This review emphasized the recent research findings on the application of INPs in medical imaging and therapeutic of cancers. The current challenges and the perspectives for their future applications were also represented and discussed.
{"title":"A review on theranostic applications of iodine nanoparticles: Recent findings and perspectives","authors":"M. Mansouri, D. Shahbazi-Gahrouei","doi":"10.22038/NMJ.2021.56425.1575","DOIUrl":"https://doi.org/10.22038/NMJ.2021.56425.1575","url":null,"abstract":"Application of nanoparticles have in the core of researchers attention for both imaging and therapy of cancers. This review article aimed to prepare an outline on recent applications of iodine nanoparticles (INPs) as theranostic agents in both diagnosis and therapies. Among various strategies are used in treatment of cancers, radiotherapy with radiopharmaceutical agents especially radioisotope of iodine displays satisfactory results for numerous types of cancers. In recent years, new investigations were done in order to develop the novel structure of INPs. These nanoprobes could act as efficient theranostic purposes. Iodine nanoparticles may be applied in nuclear medicine imaging and may be effective with mega voltage (MV) photons in cancer therapy, but this remains to be tested with different cancer cells. By using INPs, effective steps can be taken in the future in both diagnosis and treatment of cancers. This review emphasized the recent research findings on the application of INPs in medical imaging and therapeutic of cancers. The current challenges and the perspectives for their future applications were also represented and discussed.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48091886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-14DOI: 10.22038/NMJ.2021.57263.1590
Mohadesse Mirshekari, A. B. Ghomi, A. Mehravaran
Terbinafine (TBF) is a drug with well-known antifungal properties effective against skin dermatophyte infections and nail particularly in treatment of pityriasis (tinea) vesicolor due to Malassezia furfur. Terbinafine topical administration is often recommended because commercial conventional terbinafine hydrochloride tablets are more expensive and have potential for significant adverse effects. Only less than 5% of terbinafine is absorbed in conventional topical forms. Novel nano-formulation approaches would be an efficient way to enhance penetration and abortion of topical drugs and eliminate limitations of conventional drug delivery systems. As conclusion, we believe that administering the Terbinafine in nano-formulations, according to different studies, could increases penetration of TBF through stratum corneum and viable epidermis and light the path of nano-structural delivery system in clinical application. Present overview aims to evaluate nano-strategies applied to improve permeation profile and terbinafine skin delivery.
{"title":"Smart terbinafine recent nano-advances in delivery of terbinafine","authors":"Mohadesse Mirshekari, A. B. Ghomi, A. Mehravaran","doi":"10.22038/NMJ.2021.57263.1590","DOIUrl":"https://doi.org/10.22038/NMJ.2021.57263.1590","url":null,"abstract":"Terbinafine (TBF) is a drug with well-known antifungal properties effective against skin dermatophyte infections and nail particularly in treatment of pityriasis (tinea) vesicolor due to Malassezia furfur. Terbinafine topical administration is often recommended because commercial conventional terbinafine hydrochloride tablets are more expensive and have potential for significant adverse effects. Only less than 5% of terbinafine is absorbed in conventional topical forms. Novel nano-formulation approaches would be an efficient way to enhance penetration and abortion of topical drugs and eliminate limitations of conventional drug delivery systems. As conclusion, we believe that administering the Terbinafine in nano-formulations, according to different studies, could increases penetration of TBF through stratum corneum and viable epidermis and light the path of nano-structural delivery system in clinical application. Present overview aims to evaluate nano-strategies applied to improve permeation profile and terbinafine skin delivery.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44503309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-01DOI: 10.22038/NMJ.2021.56952.1581
N. Bavarsad, N. S. Karampour, G. Hemmati, A. Rezaie
Objective(s) Quercetin antioxidant properties could play an important role in various fields of health. However, its use has been limited because of several disadvantages such as very low solubility in water and high instability in the presence of air, light and heat. Encapsulation of quercetin in nanostructure systems such as liposome may lead to decrease the adverse effects and protect this molecule against degradation. The aim of this study was preparation and in-vitro and in-vivo evaluation of liposomes for topical delivery of quercetin to improve the pressure ulcers.Materials and Methods Liposomal formulations were prepared by fusion method and characterized. The amount of drug retained in and penetrated through mouse skin after 8 hours were determined. Also microscopic and macroscopic examination of laboratory animals was performed.Results Encapsulation efficacy of liposomes was in range 64.66-77.83%. Formulation F4 showed maximum drug release in 8 hours and the remaining drug in the skin layers was more than 46%. Histological investigation suggested that F4 and phenytoin 1% cream have the healing effect on the pressure ulcer during 28 day-treatment.Conclusion Quercetin liposomes due to its natural structure and minimal systemic absorption and side effects can be a suitable candidate for the treatment of pressure ulcers.
{"title":"The effect of topical quercetin loaded liposome on pressure ulcer healing in rats","authors":"N. Bavarsad, N. S. Karampour, G. Hemmati, A. Rezaie","doi":"10.22038/NMJ.2021.56952.1581","DOIUrl":"https://doi.org/10.22038/NMJ.2021.56952.1581","url":null,"abstract":"Objective(s) Quercetin antioxidant properties could play an important role in various fields of health. However, its use has been limited because of several disadvantages such as very low solubility in water and high instability in the presence of air, light and heat. Encapsulation of quercetin in nanostructure systems such as liposome may lead to decrease the adverse effects and protect this molecule against degradation. The aim of this study was preparation and in-vitro and in-vivo evaluation of liposomes for topical delivery of quercetin to improve the pressure ulcers.Materials and Methods Liposomal formulations were prepared by fusion method and characterized. The amount of drug retained in and penetrated through mouse skin after 8 hours were determined. Also microscopic and macroscopic examination of laboratory animals was performed.Results Encapsulation efficacy of liposomes was in range 64.66-77.83%. Formulation F4 showed maximum drug release in 8 hours and the remaining drug in the skin layers was more than 46%. Histological investigation suggested that F4 and phenytoin 1% cream have the healing effect on the pressure ulcer during 28 day-treatment.Conclusion Quercetin liposomes due to its natural structure and minimal systemic absorption and side effects can be a suitable candidate for the treatment of pressure ulcers.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48950019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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