Development and validation of a quick assay for the total glucosinolate content in horseradish (Armoracia rusticana) using glucose strips and a blood glucose meter.
{"title":"Development and validation of a quick assay for the total glucosinolate content in horseradish (Armoracia rusticana) using glucose strips and a blood glucose meter.","authors":"N. Meitinger, W. Kreis","doi":"10.5073/JABFQ.2018.091.031","DOIUrl":null,"url":null,"abstract":"A quick assay to determine the total glucosinolate content of fresh horseradish roots in less than 10 minutes is described. The method involves the following steps: 1. Maceration of horseradish root with 4% phosphoric acid to avoid enzymatic degradation of endogenous glucosinolates, 2. neutralization of the extract and determination of free glucose using a commercial blood glucose meter, 3. enzymatic hydrolysis of the glucosinolates by exogenous myrosinase, 4. detection of released glucose, again using a blood glucose meter, and 5. calculation of the glucosinolate content on the basis of the difference between the two glucose values determined. The newly developed assay (‘ITC quick test’) was compared with a standard high-performance liquid chromatographic method for glucosinolate analysis.","PeriodicalId":56276,"journal":{"name":"Journal of Applied Botany and Food Quality-Angewandte Botanik","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2018-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Botany and Food Quality-Angewandte Botanik","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.5073/JABFQ.2018.091.031","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 1
Abstract
A quick assay to determine the total glucosinolate content of fresh horseradish roots in less than 10 minutes is described. The method involves the following steps: 1. Maceration of horseradish root with 4% phosphoric acid to avoid enzymatic degradation of endogenous glucosinolates, 2. neutralization of the extract and determination of free glucose using a commercial blood glucose meter, 3. enzymatic hydrolysis of the glucosinolates by exogenous myrosinase, 4. detection of released glucose, again using a blood glucose meter, and 5. calculation of the glucosinolate content on the basis of the difference between the two glucose values determined. The newly developed assay (‘ITC quick test’) was compared with a standard high-performance liquid chromatographic method for glucosinolate analysis.
期刊介绍：
The Journal of Applied Botany and Food Quality is the Open Access journal of the German Society for Quality Research on Plant Foods and the Section Applied Botany of the German Botanical Society. It provides a platform for scientists to disseminate recent results of applied plant research in plant physiology and plant ecology, plant biotechnology, plant breeding and cultivation, phytomedicine, plant nutrition, plant stress and resistance, plant microbiology, plant analysis (including -omics techniques), and plant food chemistry. The articles have a clear focus on botanical and plant quality aspects and contain new and innovative information based on state-of-the-art methodologies.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
