Identification of grapevine virus G and grapevine virus H in Sardinia, Italy

IF 1.9 3区农林科学 Q2 AGRONOMY Phytopathologia Mediterranea Pub Date : 2023-01-13 DOI:10.36253/phyto-13619

N. Schianchi, Sandro Flore, Martin Jagunić, S. Serra, V. Prota, D. Vončina

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引用次数: 1

Abstract

Grapevine virus G (GVG) and grapevine virus H (GVH) (genus Vitivirus) are recently discovered viruses. Analysis of 38 samples from Sardinian grapevine cultivars for the presence of GVG and GVH was carried out using RT-PCR. All samples were also tested for grapevine Pinot gris virus (GPGV) using RT-PCR, and for grapevine leafroll virus -1, -2 and -3, grapevine virus A (GVA) and B (GVB), arabis mosaic virus (ArMV), grapevine fanleaf virus (GFLV) and grapevine fleck virus (GFkV) using multiplex RT-PCR. GVG was confirmed in four vines, and GVH was detected in only one sample. In phylogenetic analyses of the coat protein (CP) region, the Sardinian GVG isolates clustered separately from isolates from Croatia and New Zealand. The Sardinian GVH isolate clustered with most sequences from other countries, but with greater affinity to isolates from California (USA) for the CP region, whereas it clustered with isolates from Croatia in the RNA-dependent RNA polymerase (RdRp) region. In addition to GVG and GVH, many samples were coinfected with GVA, viruses from the leafroll complex, and GPGV. This is the first record of GVG and GVH occurring in Italy.

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意大利撒丁岛葡萄病毒G和H的鉴定

葡萄病毒G（GVG）和葡萄病毒H（GVH）（葡萄病毒属）是最近发现的病毒。使用RT-PCR对来自撒丁岛葡萄品种的38个样品进行了GVG和GVH的存在分析。所有样品还使用RT-PCR检测葡萄灰皮诺病毒（GPGV），并使用多重RT-PCR检测葡萄卷叶病毒-1、-2和-3、葡萄病毒A（GVA）和B（GVB）、阿拉伯马赛克病毒（ArMV）、葡萄扇叶病毒（GFLV）和葡萄斑点病毒（GFkV）。GVG在四个葡萄藤中得到证实，而GVH仅在一个样本中检测到。在外壳蛋白（CP）区域的系统发育分析中，撒丁岛GVG分离株与克罗地亚和新西兰的分离株分离。撒丁岛GVH分离株与其他国家的大多数序列聚在一起，但在CP区与来自加利福尼亚州（美国）的分离株具有更大的亲和力，而在RNA依赖性RNA聚合酶（RdRp）区与来自克罗地亚的分离株聚在一起。除了GVG和GVH，许多样本还与GVA、来自卷叶复合体的病毒和GPGV共同感染。这是意大利出现的GVG和GVH的第一个记录。

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来源期刊

Phytopathologia Mediterranea 生物-植物科学

CiteScore

4.40

自引率

8.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Phytopathologia Mediterranea is an international journal edited by the Mediterranean Phytopathological Union. The journal’s mission is the promotion of plant health for Mediterranean crops, climate and regions, safe food production, and the transfer of new knowledge on plant diseases and their sustainable management. The journal deals with all areas of plant pathology, including etiology, epidemiology, disease control, biochemical and physiological aspects, and utilization of molecular technologies. All types of plant pathogens are covered, including fungi, oomycetes, nematodes, protozoa, bacteria, phytoplasmas, viruses, and viroids. The journal also gives a special attention to research on mycotoxins, biological and integrated management of plant diseases, and the use of natural substances in disease and weed control. The journal focuses on pathology of Mediterranean crops grown throughout the world. The Editorial Board of Phytopathologia Mediterranea has recently been reorganised, under two Editors-in-Chief and with an increased number of editors.