Reno-protective Effect of Citrus sinensis by Regulating Antioxidant Capability and Gene Expression in Adenine-Induced Chronic Kidney in Rats

Q3 Biochemistry, Genetics and Molecular Biology Biointerface Research in Applied Chemistry Pub Date : 2022-09-17 DOI:10.33263/briac134.349

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Abstract

This study was performed to evaluate the reno-protective and antioxidant effects of aqueous Citrus sinensis (orange) peel extract in male Wistar rats administrated adenine. Adenine was administrated to male Wistar rats at a dose of 0.75 percent w/w in feed daily for four weeks. Rats previously fed adenine were treated with orange peel extract at a dose of 40 mg/kg BW/day by oral gavage during the same period of adenine administration. The results indicate that rats fed adenine- with aqueous orange peel extract significantly decreased urea, creatinine, and uric acid concentrations in the blood. The elevated kidney lipid peroxidation product, the lowered glutathione concentration, and the suppressed antioxidant enzyme activities were significantly improved. At the same time, the expressions of heme-oxygenase1 (HO-1), wnt7a, and β-catenin genes were actively regulated. Histological changes in the kidney were represented by congestion, hypertrophied glomerulus, vacuolization of the endothelial cells lining the glomerular tuft, and interstitial nephritis in adenine-administrated rats also remarkably improved. In conclusion, administering orange peel aqueous extract with adenine reduces the signs of adenine toxicity on renal function by enhancing the antioxidant defense system.

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柑桔通过调节腺嘌呤诱导的大鼠慢性肾脏的抗氧化能力和基因表达对肾的保护作用

本研究旨在评价柑橘皮提取物对雄性Wistar大鼠腺嘌呤的保护和抗氧化作用。雄性Wistar大鼠每天以0.75%w/w的饲料剂量给予腺嘌呤，持续四周。先前喂食腺嘌呤的大鼠在腺嘌呤给药的同一时间段内，以40mg/kg BW/天的剂量经口灌胃用橙皮提取物处理。结果表明，喂食腺嘌呤和陈皮水提取物的大鼠显著降低了血液中的尿素、肌酐和尿酸浓度。升高的肾脏脂质过氧化产物、降低的谷胱甘肽浓度和抑制的抗氧化酶活性都得到了显著改善。同时，血红素加氧酶1（HO-1）、wnt7a和β-catenin基因的表达受到积极调控。肾脏的组织学变化表现为充血、肾小球肥大、肾小球簇内皮细胞空泡化，腺嘌呤给药大鼠的间质性肾炎也显著改善。总之，服用含有腺嘌呤的橙皮水提取物可以通过增强抗氧化防御系统来减少腺嘌呤对肾功能的毒性迹象。

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来源期刊

Biointerface Research in Applied Chemistry CHEMISTRY, APPLIED-

CiteScore

4.80

自引率

0.00%

发文量

256

期刊介绍： Biointerface Research in Applied Chemistry is an international and interdisciplinary research journal that focuses on all aspects of nanoscience, bioscience and applied chemistry. Submissions are solicited in all topical areas, ranging from basic aspects of the science materials to practical applications of such materials. With 6 issues per year, the first one published on the 15th of February of 2011, Biointerface Research in Applied Chemistry is an open-access journal, making all research results freely available online. The aim is to publish original papers, short communications as well as review papers highlighting interdisciplinary research, the potential applications of the molecules and materials in the bio-field. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible.