Studies on sexual compatibility and or incompatibility among Psidium species and their hybridity confirmation through ssr markers

IF 0.9 4区 生物学 Q4 PLANT SCIENCES Israel Journal of Plant Sciences Pub Date : 2021-02-03 DOI:10.1163/22238980-BJA10018
J. Mulagund, M. Dinesh, C. Vasugi, A. Rekha, K. Ravishankar
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引用次数: 1

Abstract

The present investigation was carried out with an aim to determine the cross compatibility between Psidium species (P. chinensis, P. guineense, P. cattleianum and P. molle) and commercial guava cultivars (Arka Kiran, Arka Rashmi, Arka Mridula and Allahabad Safed). The results revealed that, P. chinensis and P. guineense were found to be good cross compatible male parents whereas, Allahabad Safeda and Arka Kiran were found to be good cross compatible female parents. However, no fruit set and seed set were obtained when P. cattleianum and P. molle were used as male parent indicating the cross incompatibility of these species with commercial guava cultivars which is reconfirmed using florescence microscopy technique to understand the pollen-pistil interaction between incompatible P. cattleianum and P. molle species with P. guajava cv. Allahabad Safeda. Result indicated that low fluorescence intensity along with poor pollen germination and improper pollen-tube growth could be the reason for incompatibility. In order to break this incompatibility, a modified pollination technique called stigma maceration technique was followed wherein stigma of the male parent macerated with different chemicals viz., carbohydrate source (2% sucrose), growth regulators (50 ppm GA3 and iaa) and micronutrients (50 ppm of each Boric acid, MgSO4, CaNO3 and KNO3) and pollinated twice i.e., on the day of flower opening and the same time of next day resulted in cross compatibility of P. cattleianum and P. molle with commercial guava cultivars. Further hybridity confirmation of interspecific hybrids using ssr markers revealed that, 8 markers were found to be co-dominant and confirmed the hybridity of interspecific hybrid combinations.
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紫堇属植物的性亲和性与性不亲和性及其ssr标记鉴定
本研究旨在确定番石榴属番石榴(P. chinensis、P. guineense、P. cattleanum和P. molle)与商业番石榴品种(Arka Kiran、Arka Rashmi、Arka Mridula和Allahabad Safed)的杂交亲和性。结果表明,中国稻和几内亚稻是优良的杂交亲本,阿拉哈巴德Safeda和Arka Kiran是优良的杂交亲本。然而,当以牛菖蒲和毛菖蒲为父本时,没有结实和结实的结果,表明这些物种与商业番石榴品种杂交不亲和,利用荧光显微镜技术重新证实了这一点,以了解不亲和的牛菖蒲和毛菖蒲与番石榴的花粉-雌蕊相互作用。阿拉哈巴德Safeda。结果表明,荧光强度低、花粉萌发差、花粉管生长不正常可能是不亲和的原因。为了打破这种不亲和性,采用了一种改良的授粉技术,即柱头浸渍技术,将父本的柱头浸渍不同的化学物质,即碳水化合物源(2%蔗糖),生长调节剂(50 ppm GA3和iaa)和微量营养素(50 ppm硼酸,MgSO4, CaNO3和KNO3),并授粉两次,即。在开花当天和第二天同一时间进行杂交处理,结果表明,牛番石榴和毛番石榴与商品番石榴品种杂交亲和性较好。进一步利用ssr标记对种间杂交种进行杂种性鉴定,发现8个标记共显性,证实了种间杂交种组合的杂种性。
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来源期刊
Israel Journal of Plant Sciences
Israel Journal of Plant Sciences 生物-植物科学
CiteScore
1.90
自引率
0.00%
发文量
17
审稿时长
>12 weeks
期刊介绍: The Israel Journal of Plant Sciences is an international journal of extensive scope that publishes special issues dealing with all aspects of plant sciences, including but not limited to: physiology, cell biology, development, botany, genetic
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