Viability and fertility of cryopreserved pollen of Brinjal and its wild relatives

IF 0.9 4区 生物学 Q4 PLANT SCIENCES Israel Journal of Plant Sciences Pub Date : 2023-07-12 DOI:10.1163/22238980-bja10084
P. Rajasekharan, Harsha Ravi
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引用次数: 1

Abstract

The Solanum wild relatives are valuable for developing pest and disease-resistant lines in the breeding program of S. melongena (brinjal). However, breeding new varieties that are superior to the existing ones in S. melongena faces several challenges. To overcome geographical and seasonal barriers, a breeding strategy involving pollen cryopreservation may be helpful. Fifteen different Solanum species/accessions of wild relatives and two accessions from cultivated species were studied for in vitro germination and fertility. Among these S. indicum accessions exhibited the highest fresh pollen germination of 92.7 ± 0.7 (JRPH/15–154) and 88.2 ± 3.2% after 1 year and 4 months of cryostorage. In vivo fertility was estimated by quantifying fruit set after pollination with both fresh and cryopreserved pollen. The cryopreserved pollen was able to set fruit (91.5 ± 0.5 in JRPH/15–154) compared to fresh pollen (100%). Scanning Electron Microscopic studies (SEM) were conducted on both fresh and cryopreserved pollen, and various morphological parameters such as pollen length, breadth, and perimeter were recorded for all the accessions and species. These studies explore the possibility of establishing a pollen cryobank for Solanum wild species.
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茄子及其野生近缘种低温保存花粉的活力和育性
茄属野生近缘种在茄属茄子选育中具有重要的抗病、病虫害选育价值。然而,培育优于现有品种的龙葵新品种面临着一些挑战。为了克服地理和季节障碍,一种涉及花粉冷冻保存的繁殖策略可能会有所帮助。研究了15个野生近缘种和2个栽培种的茄属植物的离体萌发和育性。其中,贮藏1年4个月的籼稻花粉萌发率最高,分别为92.7±0.7 (JRPH/ 15-154)和88.2±3.2%。用新鲜和低温保存的花粉授粉后,通过定量结果来估计体内育性。在JRPH/ 15-154条件下,低温保存花粉的结实率为91.5±0.5,而新鲜花粉的结实率为100%。对新鲜和冷冻保存的花粉进行了扫描电镜(SEM)研究,记录了所有材料和物种的花粉长度、宽度和周长等形态学参数。本研究探讨了建立野生茄属植物花粉冷冻库的可能性。
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来源期刊
Israel Journal of Plant Sciences
Israel Journal of Plant Sciences 生物-植物科学
CiteScore
1.90
自引率
0.00%
发文量
17
审稿时长
>12 weeks
期刊介绍: The Israel Journal of Plant Sciences is an international journal of extensive scope that publishes special issues dealing with all aspects of plant sciences, including but not limited to: physiology, cell biology, development, botany, genetic
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