Viability and fertility of cryopreserved pollen of Brinjal and its wild relatives

IF 0.9 4区 生物学 Q4 PLANT SCIENCES Israel Journal of Plant Sciences Pub Date : 2023-07-12 DOI:10.1163/22238980-bja10084
P. Rajasekharan, Harsha Ravi
{"title":"Viability and fertility of cryopreserved pollen of Brinjal and its wild relatives","authors":"P. Rajasekharan, Harsha Ravi","doi":"10.1163/22238980-bja10084","DOIUrl":null,"url":null,"abstract":"\nThe Solanum wild relatives are valuable for developing pest and disease-resistant lines in the breeding program of S. melongena (brinjal). However, breeding new varieties that are superior to the existing ones in S. melongena faces several challenges. To overcome geographical and seasonal barriers, a breeding strategy involving pollen cryopreservation may be helpful. Fifteen different Solanum species/accessions of wild relatives and two accessions from cultivated species were studied for in vitro germination and fertility. Among these S. indicum accessions exhibited the highest fresh pollen germination of 92.7 ± 0.7 (JRPH/15–154) and 88.2 ± 3.2% after 1 year and 4 months of cryostorage. In vivo fertility was estimated by quantifying fruit set after pollination with both fresh and cryopreserved pollen. The cryopreserved pollen was able to set fruit (91.5 ± 0.5 in JRPH/15–154) compared to fresh pollen (100%). Scanning Electron Microscopic studies (SEM) were conducted on both fresh and cryopreserved pollen, and various morphological parameters such as pollen length, breadth, and perimeter were recorded for all the accessions and species. These studies explore the possibility of establishing a pollen cryobank for Solanum wild species.","PeriodicalId":14689,"journal":{"name":"Israel Journal of Plant Sciences","volume":" ","pages":""},"PeriodicalIF":0.9000,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Israel Journal of Plant Sciences","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1163/22238980-bja10084","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 1

Abstract

The Solanum wild relatives are valuable for developing pest and disease-resistant lines in the breeding program of S. melongena (brinjal). However, breeding new varieties that are superior to the existing ones in S. melongena faces several challenges. To overcome geographical and seasonal barriers, a breeding strategy involving pollen cryopreservation may be helpful. Fifteen different Solanum species/accessions of wild relatives and two accessions from cultivated species were studied for in vitro germination and fertility. Among these S. indicum accessions exhibited the highest fresh pollen germination of 92.7 ± 0.7 (JRPH/15–154) and 88.2 ± 3.2% after 1 year and 4 months of cryostorage. In vivo fertility was estimated by quantifying fruit set after pollination with both fresh and cryopreserved pollen. The cryopreserved pollen was able to set fruit (91.5 ± 0.5 in JRPH/15–154) compared to fresh pollen (100%). Scanning Electron Microscopic studies (SEM) were conducted on both fresh and cryopreserved pollen, and various morphological parameters such as pollen length, breadth, and perimeter were recorded for all the accessions and species. These studies explore the possibility of establishing a pollen cryobank for Solanum wild species.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
茄子及其野生近缘种低温保存花粉的活力和育性
茄属野生近缘种在茄属茄子选育中具有重要的抗病、病虫害选育价值。然而,培育优于现有品种的龙葵新品种面临着一些挑战。为了克服地理和季节障碍,一种涉及花粉冷冻保存的繁殖策略可能会有所帮助。研究了15个野生近缘种和2个栽培种的茄属植物的离体萌发和育性。其中,贮藏1年4个月的籼稻花粉萌发率最高,分别为92.7±0.7 (JRPH/ 15-154)和88.2±3.2%。用新鲜和低温保存的花粉授粉后,通过定量结果来估计体内育性。在JRPH/ 15-154条件下,低温保存花粉的结实率为91.5±0.5,而新鲜花粉的结实率为100%。对新鲜和冷冻保存的花粉进行了扫描电镜(SEM)研究,记录了所有材料和物种的花粉长度、宽度和周长等形态学参数。本研究探讨了建立野生茄属植物花粉冷冻库的可能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Israel Journal of Plant Sciences
Israel Journal of Plant Sciences 生物-植物科学
CiteScore
1.90
自引率
0.00%
发文量
17
审稿时长
>12 weeks
期刊介绍: The Israel Journal of Plant Sciences is an international journal of extensive scope that publishes special issues dealing with all aspects of plant sciences, including but not limited to: physiology, cell biology, development, botany, genetic
期刊最新文献
Evaluation of morpho-physiological and biochemical indicators of salinity stress in Argemone mexicana L., medicinal plant from Papaveraceae Effect of salt stress on morpho-physiological and anatomical attributes of Salix clones Enhancement of salt tolerance of transgenic Arabidopsis thaliana by Zostera japonica DnaJ gene Viability and fertility of cryopreserved pollen of Brinjal and its wild relatives Studies on soil physico-chemical properties and biochemical composition of selected vegetables collected from locally irrigated farmland with municipal wastewater in Minna, Niger State, Nigeria
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1