{"title":"Issue Information TOC","authors":"","doi":"10.1002/cpim.67","DOIUrl":null,"url":null,"abstract":"<p><b>Cover</b>: In Geddes-McAlister and Gadjeva (https://doi.org/10.1002/cpim.87), Overview of workflow for MS-based proteomics profiling of neutrophils. Neutrophils are isolated and purified from bone marrow of 7- to 9-week-old male or female mice. The quality of the purification is assessed by FACS. Proteins are extracted by chemical (e.g., urea) and mechanical (e.g., sonication) cell disruption and enzymatically digested with Lys-C and trypsin proteases. The purified peptides are measured in the first MS scan and peptide fragmentation patterns are observed in the second MS scan (MS/;MS). The data is processed and analyzed using the publicly available MaxQuant and Perseus platforms. Figure generated with Biorender.com. See e87.\n\n <figure>\n <div><picture>\n <source></source></picture><p></p>\n </div>\n </figure></p>","PeriodicalId":10733,"journal":{"name":"Current Protocols in Immunology","volume":"126 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpim.67","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Immunology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpim.67","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Immunology and Microbiology","Score":null,"Total":0}
引用次数: 0
Abstract
Cover: In Geddes-McAlister and Gadjeva (https://doi.org/10.1002/cpim.87), Overview of workflow for MS-based proteomics profiling of neutrophils. Neutrophils are isolated and purified from bone marrow of 7- to 9-week-old male or female mice. The quality of the purification is assessed by FACS. Proteins are extracted by chemical (e.g., urea) and mechanical (e.g., sonication) cell disruption and enzymatically digested with Lys-C and trypsin proteases. The purified peptides are measured in the first MS scan and peptide fragmentation patterns are observed in the second MS scan (MS/;MS). The data is processed and analyzed using the publicly available MaxQuant and Perseus platforms. Figure generated with Biorender.com. See e87.
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