Estudio de los efectos de un microambiente hipóxico en queratinocitos humanos in vitro y su correlato con alteraciones del microambiente en la patología de liquen plano oral

IF 0.2 Q4 DENTISTRY, ORAL SURGERY & MEDICINE Odontoestomatologia Pub Date : 2023-03-15 DOI:10.22592/ode2022n40e223
Natali D´ AIUTO
{"title":"Estudio de los efectos de un microambiente hipóxico en queratinocitos humanos in vitro y su correlato con alteraciones del microambiente en la patología de liquen plano oral","authors":"Natali D´ AIUTO","doi":"10.22592/ode2022n40e223","DOIUrl":null,"url":null,"abstract":"Hypoxia is a fundamental factor in the process of tumor genesis, as well as in precursor pathologies of cancer, such as Oral Lichen Planus (OLP). Objective: To determine if it is possible to establish a correlation between the alterations that normal keratinocytes suffer in a hypoxic microenvironment in vitro and alterations that appear in the keratinocytes in the epithelium of the oral mucosa in the context of OLP pathology. Methods: Morphological changes were studied by phase contrast microscopy, and the detection of markers associated with hypoxia of human keratinocytes (HaCaT), as an oral cell model, in a hypoxic microenvironment generated by the variant of the method “Hypoxia induced by coverslips”. Results: Using confocal microscopy, the presence of hypoxia markers GLUT-1 and Hi-poxyprobe was observed in HaCaT cell cultures exposed to a hypoxic microenvironment. In addition, the presence of the GLUT-1 marker was observed by immunohistochemistry in human epithelial tissue derived from biopsies of OLP pathology. Conclusions: A correlation was established between the alterations detected in human keratinocytes induced in a hypoxic microenvironment in vitro and the alterations detected in vivo in epithelial tissue of the oral mucosa.","PeriodicalId":41109,"journal":{"name":"Odontoestomatologia","volume":" ","pages":""},"PeriodicalIF":0.2000,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Odontoestomatologia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22592/ode2022n40e223","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0

Abstract

Hypoxia is a fundamental factor in the process of tumor genesis, as well as in precursor pathologies of cancer, such as Oral Lichen Planus (OLP). Objective: To determine if it is possible to establish a correlation between the alterations that normal keratinocytes suffer in a hypoxic microenvironment in vitro and alterations that appear in the keratinocytes in the epithelium of the oral mucosa in the context of OLP pathology. Methods: Morphological changes were studied by phase contrast microscopy, and the detection of markers associated with hypoxia of human keratinocytes (HaCaT), as an oral cell model, in a hypoxic microenvironment generated by the variant of the method “Hypoxia induced by coverslips”. Results: Using confocal microscopy, the presence of hypoxia markers GLUT-1 and Hi-poxyprobe was observed in HaCaT cell cultures exposed to a hypoxic microenvironment. In addition, the presence of the GLUT-1 marker was observed by immunohistochemistry in human epithelial tissue derived from biopsies of OLP pathology. Conclusions: A correlation was established between the alterations detected in human keratinocytes induced in a hypoxic microenvironment in vitro and the alterations detected in vivo in epithelial tissue of the oral mucosa.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
体外低氧微环境对人角化细胞的影响及其与口腔扁平地衣病理微环境改变的相关性研究
缺氧是肿瘤发生过程中的一个基本因素,也是癌症的前兆病理,如口腔扁平苔藓(OLP)。目的:确定是否有可能建立正常角质形成细胞在体外缺氧微环境中所遭受的改变与口腔黏膜上皮角质形成细胞中出现的改变之间的相关性。方法:通过相差显微镜研究形态学变化,并检测作为口腔细胞模型的人角质形成细胞(HaCaT)在“盖玻片缺氧诱导”方法产生的缺氧微环境中的缺氧相关标志物。结果:利用共聚焦显微镜,在暴露于缺氧微环境的HaCaT细胞培养物中观察到缺氧标记物GLUT-1和Hi-环氧探针的存在。此外,通过免疫组织化学在来源于OLP病理活检的人类上皮组织中观察到GLUT-1标记物的存在。结论:在体外缺氧微环境中诱导的人角质形成细胞中检测到的变化与在体内口腔粘膜上皮组织中检测到变化之间建立了相关性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Odontoestomatologia
Odontoestomatologia DENTISTRY, ORAL SURGERY & MEDICINE-
自引率
33.30%
发文量
19
审稿时长
20 weeks
期刊最新文献
Asociación entre obesidad y caries dental en niños y adolescentes. Revisión Una aplicación web de promoción de salud para capacitación virtual de maestros Sialometaplasia Necrotizante y la importancia de considerarla como un diagnóstico diferencial de neoplasias intraorales Manejo de pacientes con trastornos del neurodesarrollo en la consulta odontológica. Una revisión de la literatura Caries dental en preescolares: estudio transversal de base poblacional en Montevideo, Uruguay
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1