Effects of exposure of rat erythrocytes to a hypogeomagnetic field

IF 0.3 Q4 SPECTROSCOPY Biomedical Spectroscopy and Imaging Pub Date : 2019-01-24 DOI:10.3233/BSI-180181

A. Nadeev, Terpilowski, V. Bogdanov, D. A. Khmelevskoy, B. F. Schegolev, S. Surma, V. E. Stefanov, N. V. Goncharov, R. Jenkins

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Abstract

BACKGROUND: Hypomagnetic fields can disrupts the normal functioning of living organisms by a mechanism thought to involve oxidative stress. In erythrocytes, oxidative stress can inter alia lead to changes to hemoglobin content and to hemolysis. OBJECTIVE: To study the effects of hypomagnetism on the state of rat erythrocytes in vitro. METHODS: Rat erythrocytes were exposed to an attenuated magnetic field (AMF) or Earth’s magnetic field (EMF), in the presence of tert-butyl hydroperoxide (TBHP) as inducer of oxidative stress. Determinations: total hemoglobin (and its three forms oxyhemoglobin, methemoglobin, and hemichrome) released from erythrocytes, spectral data (500-700nm); oxygen radical concentrations, electron paramagnetic resonance. RESULTS: AMF and EMF exposed erythrocytes were compared. After 4h incubation at high TBHP concentrations (>700 μM), AMF exposed erythrocytes released significantly more (p<0.05) hemoglobin (Hb), mostly as methemoglobin (metHb). Conversely, after 24 h incubation at low TBHP concentrations (≤ 350 μM), EMF exposed erythrocytes released significantly more (p<0.001) hemoglobin, with metHb as a significant proportion of the total Hb. Erythrocytes exposed to AMF generated more radicals than those exposed to the EMF. CONCLUSION: Under particular conditions of oxidative stress, hypomagnetic fields can disrupt the functional state of erythrocytes and promote cell death; an additive effect is

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大鼠红细胞暴露于低地磁场的影响

背景：低磁场可以通过一种被认为与氧化应激有关的机制破坏生物体的正常功能。在红细胞中，氧化应激可导致血红蛋白含量的变化和溶血。目的：研究低磁性对体外培养大鼠红细胞状态的影响。方法：将大鼠红细胞暴露于弱磁场（AMF）或地球磁场（EMF）中，以叔丁基氢过氧化物（TBHP）作为氧化应激的诱导剂。测定：红细胞释放的总血红蛋白（及其三种形式的氧合血红蛋白、高铁血红蛋白和半色素），光谱数据（500-700nm）；氧自由基浓度、电子顺磁共振。结果：比较AMF和EMF暴露的红细胞。在高TBHP浓度（>700μM）下孵育4h后，暴露于AMF的红细胞释放出显著更多（p<0.05）的血红蛋白（Hb），主要是高铁血红蛋白（metHb）。相反，在低TBHP浓度（≤350μM）下孵育24小时后，暴露于EMF的红细胞释放出显著更多（p＜0.001）的血红蛋白，metHb占总Hb的显著比例。暴露于AMF的红细胞比暴露于EMF的红细胞产生更多的自由基。结论：在特定的氧化应激条件下，低磁场可破坏红细胞的功能状态，促进细胞死亡；相加效应是

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Biomedical Spectroscopy and Imaging SPECTROSCOPY-

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期刊介绍： Biomedical Spectroscopy and Imaging (BSI) is a multidisciplinary journal devoted to the timely publication of basic and applied research that uses spectroscopic and imaging techniques in different areas of life science including biology, biochemistry, biotechnology, bionanotechnology, environmental science, food science, pharmaceutical science, physiology and medicine. Scientists are encouraged to submit their work for publication in the form of original articles, brief communications, rapid communications, reviews and mini-reviews. Techniques covered include, but are not limited, to the following: • Vibrational Spectroscopy (Infrared, Raman, Teraherz) • Circular Dichroism Spectroscopy • Magnetic Resonance Spectroscopy (NMR, ESR) • UV-vis Spectroscopy • Mössbauer Spectroscopy • X-ray Spectroscopy (Absorption, Emission, Photoelectron, Fluorescence) • Neutron Spectroscopy • Mass Spectroscopy • Fluorescence Spectroscopy • X-ray and Neutron Scattering • Differential Scanning Calorimetry • Atomic Force Microscopy • Surface Plasmon Resonance • Magnetic Resonance Imaging • X-ray Imaging • Electron Imaging • Neutron Imaging • Raman Imaging • Infrared Imaging • Terahertz Imaging • Fluorescence Imaging • Near-infrared spectroscopy.