Rapid and Simultaneous Analysis of Seven Oral Anti-Diabetic Drugs

Abstract

The present study describes the optimization and validation of a high-performance liquid chromatographic (HPLC) method for the determination of seven medicines (Metformin hydrochloride, Vildagliptin, Gliclazide, Sitagliptin phosphate, Pioglitazone hydrochloride, Glibenclamide and Glimepirde) in bulk and pharmaceutical dosage forms. The chromatographic separation could be achieved using a C8 column (100 Å, 5 µm, 150 mm) at 30 °C. Thereby, the mobile phase is a mixture of acetonitrile and an aqueous solution of triethylamine (0.5% v/v) and phosphoric acid 10% w/v (pH 7.0) at a mixing ratio of 32.5:67.5 v/v%, with a flow rate of 1.0 ml/min and detection performed at 210 nm using a UV detector. The results were found satisfactory and the method can be adapted to routine quality control of medications as recommended by the guidelines of the International Conference on Harmonization.