Scale-Up Studies for Polyhydroxyalkanoate and Halocin Production by Halomonas Sp. as Potential Biomedical Materials

Abstract

Polyhydroxyalkanoates (PHA) are the biomaterials isolated naturally from bacterial strains. These are present in granules and accumulated intracellularly for storage and energy uptake in stressed conditions. This work was based on the extraction of polyhydroxyalkanoates from haloarchaeal strains isolated from samples of a salt mine and Halocin activity screening of these isolates. For the screening of polyhydroxyalkanoates, Nile Blue and Sudan Black Staining were performed. After confirmation and theoretical determination, polyhydroxyalkanoates extraction was done by sodium hypochlorite digestion and solvent extraction by chloroform method in combination. Polyhydroxyalkanoates production was calculated along with the determination of biomass. Halocin activity of these strains was also screened at different intervals. Isolated strains were identified by 16S RNA gene sequencing. Polyhydroxyalkanoates polymer was produced in form of biofilms and brittle crystals. Halocin activity was exhibited by four strains, among which confirmed halocin activity was shown by strain K7. The remarkable results showed that polyhydroxyalkanoates can replace synthetic plastics which are not environment friendly as they cause environmental pollution – a major threat to Earth rising gradually. Therefore, by switching to the use of biodegradable bioplastics from the use of synthetic plastics, it would be beneficial to the ecosphere.