João M. S. Cardoso, N. Yoshimoto, Shuichi Yamamoto
{"title":"Methods for Thermodynamic Analysis of Temperature Dependence of Distribution Coefficients in Chromatography Separations","authors":"João M. S. Cardoso, N. Yoshimoto, Shuichi Yamamoto","doi":"10.11301/jsfe.19550t","DOIUrl":null,"url":null,"abstract":"Thermodynamic analysis of chromatography separation is important not only for optimization of separation but also development of new separation media and processes. In this study temperature dependence in the separation of two model components (polyphenols) by polymer-based chromatography with ethanol-water mobile phase was analyzed by van’t Hoff plots and isothermal titration calorimeter (ITC). The distribution coef ficient K obtained by the isocratic elution of chromatography was employed both for van’t Hoff plots and for the calculation of adsorbed amount in the ITC measurements. Enthalpy values obtained by both methods were similar. As ITC is not able to predict separation behavior, it is recommended to use chromatography experiments as a complimentary method for analyzing the temperature dependence of separation.","PeriodicalId":39399,"journal":{"name":"Japan Journal of Food Engineering","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japan Journal of Food Engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.11301/jsfe.19550t","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Engineering","Score":null,"Total":0}
引用次数: 0
Abstract
Thermodynamic analysis of chromatography separation is important not only for optimization of separation but also development of new separation media and processes. In this study temperature dependence in the separation of two model components (polyphenols) by polymer-based chromatography with ethanol-water mobile phase was analyzed by van’t Hoff plots and isothermal titration calorimeter (ITC). The distribution coef ficient K obtained by the isocratic elution of chromatography was employed both for van’t Hoff plots and for the calculation of adsorbed amount in the ITC measurements. Enthalpy values obtained by both methods were similar. As ITC is not able to predict separation behavior, it is recommended to use chromatography experiments as a complimentary method for analyzing the temperature dependence of separation.
期刊介绍：
The Japan Society for Food Engineering (the Society) publishes "Japan Journal of Food Engineering (the Journal)" to convey and disseminate information regarding food engineering and related areas to all members of the Society as an important part of its activities. The Journal is published with an aim of gaining wide recognition as a periodical pertaining to food engineering and related areas.
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