The significance of HERC5, IFIH1, SAMD4, SEMA3A and MCTP1 genes expression in resistance to cytotoxic drugs in ovarian cancer cell lines

Q4 Biochemistry, Genetics and Molecular Biology Medical Journal of Cell Biology Pub Date : 2021-09-01 DOI:10.2478/acb-2021-0019

M. Nowacka, B. Ginter-Matuszewska, M. Świerczewska, M. Nowicki, M. Zabel, Karolina Sterzyńska, R. Januchowski

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引用次数: 2

Abstract

Abstract Resistance to chemotherapy is the main obstacle in contemporary ovarian cancer treatment. The aim of this study was the evaluation of expression of HERC5, IFIH1, SAMD4, MCTP1 and SEMA3A mRNA and assessment their role in resistance to cisplatin, paclitaxel, doxorubicin and topotecan in seven ovarian cancer cell lines. MTT assay was used in resistance assessment. Quantitative polymerase chain reaction was performed to measure the expression levels of the genes. We observed different levels of resistance among cell lines. The resistance was not related to the expression of drug transporters genes. The expression of HERC5 and IFIH1 genes was upregulated, and the expression of SEMA3A gene was downregulated. Expression of SAMD4 was upregulated in PEA1, PEA2, and PEO23 cell lines, and expression of MCTP1 was downregulated in A2780, PEA2, and PEO23 cell lines. Upregulation of HERC5, IFIH1, and SAMD4 and downregulation of SEMA3A and MCTP1 in TOP-resistant ovarian cancer cell lines may suggest some role of those genes in topotecan resistance development.

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HERC5、IFIH1、SAMD4、SEMA3A和MCTP1基因表达在卵巢癌症细胞系细胞毒性药物耐药性中的意义

摘要化疗耐药性是当代癌症治疗的主要障碍。本研究的目的是评估HERC5、IFIH1、SAMD4、MCTP1和SEMA3A mRNA的表达，并评估它们在七种卵巢癌症细胞系中对顺铂、紫杉醇、阿霉素和拓扑替康的耐药性中的作用。MTT法用于耐药性评估。进行定量聚合酶链式反应来测量基因的表达水平。我们观察到细胞系之间的抗性水平不同。耐药性与药物转运蛋白基因的表达无关。HERC5和IFIH1基因的表达上调，SEMA3A基因的表达下调。SAMD4的表达在PEA1、PEA2和PEO23细胞系中上调，MCTP1的表达在A2780、PEA2、和PEO24细胞系中下调。在TOP-耐药的卵巢癌症细胞系中，HERC5、IFIH1和SAMD4的上调以及SEMA3A和MCTP1的下调可能表明这些基因在topotecan耐药性发展中的某些作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Medical Journal of Cell Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

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