Antioxidant Activity and Polyphenols Content of Artemisia Herba-Alba Extract and their Cytotoxicity against Human Lung Cancer Cells NCI-N417

IF 1.2 Q4 PHARMACOLOGY & PHARMACY Journal of Herbs, Spices and Medicinal Plants Pub Date : 2022-04-13 DOI:10.1080/10496475.2022.2064954

S. Amkiss, Mohamed Bakha, O. Belmehdi, Fernando Carmona-Espinazo, J. López-Sáez, M. Idaomar

引用次数: 1

Abstract

ABSTRACT Phytochemicals in the ethanol extract of Artemisia herba-alba were examined and evaluated for the antioxidant and cytotoxicity potential. The extraction was performed using an accelerated solvent extraction (ASE300), analyzed by GC-MS and the total phenolics was estimated by Folin–Ciocalteau method. The antioxidant activity of the extract was evaluated using three methods and its cytotoxicity was evaluated in-vitro on NCI-N417 tumor cell line using MTT test and flow cytometry. Twenty-one compounds were identified in the A. herba-alba extract, which was rich in phenolic compounds (248.56 ± 11.05 μg GAE mg−1 extract) and had antioxidant activity characterized by a high DPPH-radical-scavenging activity (IC50 = 69.01 ± 0.03 μg mL−1), inhibitory effect of β-carotene oxidation (95.75%±0.013), and reducing power (DO700nm = 0.751 ± 0.001). Ethanol extract from A. herba-alba demonstrated cytotoxic and antiproliferative activity.

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白蒿提取物抗氧化活性、多酚含量及其对人肺癌细胞NCI-N417的细胞毒性研究

摘要:研究了白蒿乙醇提取物中的植物化学成分，并对其抗氧化和细胞毒性进行了评价。采用加速溶剂萃取(ASE300)提取，GC-MS分析，Folin-Ciocalteau法测定总酚含量。采用三种方法评价提取物的抗氧化活性，采用MTT试验和流式细胞术评价提取物对NCI-N417肿瘤细胞株的体外细胞毒性。从白刺提取物中鉴定出21个化合物，其中酚类化合物含量高(248.56±11.05 μg GAE mg−1提取物)，抗氧化活性高(IC50 = 69.01±0.03 μg mL−1)，抑制β-胡萝卜素氧化(95.75%±0.013)，还原力强(DO700nm = 0.751±0.001)。白刺乙醇提取物具有细胞毒性和抗增殖活性。

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来源期刊

Journal of Herbs, Spices and Medicinal Plants Medicine-Complementary and Alternative Medicine

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： The Journal of Herbs, Spices & Medicinal Plants is an essential reference filled with recent research and other valuable information associated with herbs, spices, and medicinal plants. The Journal serves as a focus point through which investigators and others may publish material of importance to the production, marketing, and utilization of these plants and associated extracts. The journal covers the following topics: growth, development, horticulture, ecology, physiology, genetics, chemistry, and economics. Original articles, review articles, and book reviews provide information of interest to an international audience of researchers, teachers, technicians, and managers involved with production and/or marketing of herbs, spices, and medicinal plants. Managers of food companies, food processing facilities, medical research laboratories, government agencies, and others interested in new chemicals, food additives, international trade, patents, and other items can easily review new findings. The Journal of Herbs, Spices & Medicinal Plants is a forum in which recent research and other information associated with herbs, spices, and medicinal plants is shared. The Journal represents a centralized database accessible by investigators within the international community that work with or have an interest in herbs, spices, and medicinal plants.