Expression of a Rap2.12 like-1 ERF gene during adventitious rooting of chestnut and oak microshoots

IF 0.9 4区 生物学 Q4 PLANT SCIENCES Israel Journal of Plant Sciences Pub Date : 2020-02-25 DOI:10.1163/22238980-20191119
S. Valladares, E. Varas, J. Vielba, N. Vidal, V. Codesido, R. Castro, C. Sánchez
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引用次数: 4

Abstract

Adventitious rooting of cuttings is a complex developmental process in forest species, with several exogenous and endogenous factors influencing the outcome of the process. In this study we applied an in vitro working system, comprising two lines of microshoots with the same genotype but at a different ontogenetic stages, in two different tree species (chestnut and oak). We analyzed the expression of a gene encoding an AP2/ERF transcription factor from group VII in the initial hours of the adventitious rooting induction, both in rooting competent and incompetent microshoots. The analysis revealed that expression of this gene is related to wounding, ontogenetic stage and auxin in a complex and species-specific manner. Putative induction of the gene by auxin was also analyzed in the presence of naphthyl-phthalamic acid (NPA), an auxin transport inhibitor. In situ expression analysis in chestnut relates the gene activity to cambial divisions and root primordia in rooting competent microshoots, as well as in the root apex. The putative role of the gene during adventitious roots formation is discussed.
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Rap2.12 like-1 ERF基因在板栗和栎树微梢不定根中的表达
扦插不定根是一个复杂的发育过程,受到多种外源和内源因素的影响。在这项研究中,我们应用了一个体外工作系统,包括两个具有相同基因型但处于不同个体发育阶段的微芽系,在两种不同的树种(栗子和橡树)中。我们分析了VII组AP2/ERF转录因子编码基因在不定生根诱导的最初几个小时内,在生根能力和不生根能力的微芽中表达的情况。分析表明,该基因的表达与损伤、个体发育阶段和生长素的表达有着复杂的、物种特异性的关系。在生长素运输抑制剂萘酰邻苯二甲酸(NPA)存在的情况下,还分析了生长素对该基因的诱导作用。在板栗原位表达分析中,基因活性与生根适能微芽和根尖的形成层分裂和根原基有关。讨论了该基因在不定根形成过程中可能起的作用。
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来源期刊
Israel Journal of Plant Sciences
Israel Journal of Plant Sciences 生物-植物科学
CiteScore
1.90
自引率
0.00%
发文量
17
审稿时长
>12 weeks
期刊介绍: The Israel Journal of Plant Sciences is an international journal of extensive scope that publishes special issues dealing with all aspects of plant sciences, including but not limited to: physiology, cell biology, development, botany, genetic
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