Microwave-assisted extraction of phenolic compounds from coffee (Coffea robusta L. Linden) bee pollen

Q3 Medicine Herba Polonica Pub Date : 2021-09-01 DOI:10.2478/hepo-2021-0015

L. Quoc

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引用次数: 4

Abstract

Summary Introduction: Coffee bee pollen contains some precious bioactive compounds, especially phenolic compounds. This material is easily found in many regions in Vietnam and is quite useful for human health. Objective: The main aim of this study was to determine the best extraction conditions for the total polyphenol content (TPC) and antioxidant capacity (AC) of coffee (Coffea robusta L. Linden) bee pollen with microwave-assisted extraction (MAE). Methods: TPC and AC of extract of coffee (C. robusta) bee pollen were determined using Folin-Ciocalteu (FC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. In addition, micromorphology of sample was observed using scanning electron micrographs (SEM). Results: The findings showed that all factors strongly affected the efficiency of the extraction process. The best extraction conditions obtained were ethanol concentration of 60% (v/v), solid to solvent ratio of 1/30 (w/v), extraction time of 7 min, and a microwave power of 314 W. Conclusion: The highest TPC and AC obtained were 13.73 mg GAE/g DW and 10.08 µmol TE/g DW with aqueous ethanol as the solvent at the optimal extraction condition. In addition, the microstructures of the material changed insignificantly under microwave irradiation.

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微波辅助提取咖啡花粉中的酚类化合物

简介：咖啡蜂花粉中含有一些珍贵的生物活性化合物，特别是酚类化合物。这种材料在越南的许多地区都很容易找到，对人类健康非常有用。目的：采用微波辅助提取法（MAE）测定咖啡蜂花粉中总多酚含量（TPC）和抗氧化能力（AC）的最佳提取条件。方法：采用Folin-Ciocalteu（FC）法和2，2-二苯基-1-苦基肼（DPPH）法分别测定咖啡蜂花粉提取物的TPC和AC。此外，使用扫描电子显微照片（SEM）观察样品的微观形貌。结果：结果表明，所有因素都强烈影响提取过程的效率。最佳提取条件为乙醇浓度为60%（v/v），固溶比为1/30（w/v），提取时间为7min，微波功率为314W。结论：在最佳提取条件下，以乙醇水溶液为溶剂，得到的最高TPC和AC分别为13.73mg GAE/g DW和10.08µmol TE/g DW。此外，在微波辐照下，材料的微观结构变化不大。

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