Pub Date : 2023-11-19DOI: 10.5604/01.3001.0054.0118
Justyna Baraniak, Małgorzata Kania-Dobrowolska
Introduction: Diet affects the proper function of human body and plays an important role in the preventionof many diseases. In the elderly, some body functions, including nutrition, are impaired, so it is important toconsider dietary supplements at this time. Consumption of dietary supplements by the elderly has becomecommon in recent years. In addition, the global ageing process has accelerated significantly in recent decades.Currently, on the food market, there are many dietary supplements dedicated to the elderly. Manufacturersof these products are able to perfectly recognize the special nutritional needs of the elderly, which results inthe creation of new lines of modern products.Objective: The purpose of the study was to analyse the use of dietary supplements administered in theelderly, improvement of seniors’ knowledge on dietary supplements and investigation of their awarenessabout the risks associated with such products.Methods: The study was conducted by questionnaire method among 302 elderly people in two provincesof Poland. Additionally, dietary supplements offered at various retail outlets were reviewed (53), as well aspublic warnings published by the Chief Sanitary Inspectorate in Poland that addressed the risks with dietarysupplements (10).Results: The questionnaire was completed by 302 respondents (217 females, 85 males). The age of therespondents was distributed as follows: 32.45% aged 65–74, 44. % aged 75–79, 23.17% aged ≥80. Dietarysupplements consumed by seniors or widely offered in the food market mainly include ingredients asfollows: plant preparations, vitamins and minerals, electrolytes. Significantly more women have been takingdietary supplements (72%), compared to men (36%). Simultaneous administration of two or more productswas declared by 71.9% of respondents. Unfortunately, only 21% of respondents recognized any risks ofconsuming dietary supplements.Conclusion: The use of dietary supplements among elderly people has definitely increased lately. Thequestionnaire shows that approximately 86% of respondents have consumed dietary supplements at leastonce in their life, while 14% of respondents have never used these products. The survey demonstrates thatthere is a need of the continued education of the elderly about dietary supplementation and the dangers ofuncontrolled consumption of different types of supplements.
{"title":"Pilot study of dietary supplement consumption by elderly people","authors":"Justyna Baraniak, Małgorzata Kania-Dobrowolska","doi":"10.5604/01.3001.0054.0118","DOIUrl":"https://doi.org/10.5604/01.3001.0054.0118","url":null,"abstract":"Introduction: Diet affects the proper function of human body and plays an important role in the preventionof many diseases. In the elderly, some body functions, including nutrition, are impaired, so it is important toconsider dietary supplements at this time. Consumption of dietary supplements by the elderly has becomecommon in recent years. In addition, the global ageing process has accelerated significantly in recent decades.Currently, on the food market, there are many dietary supplements dedicated to the elderly. Manufacturersof these products are able to perfectly recognize the special nutritional needs of the elderly, which results inthe creation of new lines of modern products.Objective: The purpose of the study was to analyse the use of dietary supplements administered in theelderly, improvement of seniors’ knowledge on dietary supplements and investigation of their awarenessabout the risks associated with such products.Methods: The study was conducted by questionnaire method among 302 elderly people in two provincesof Poland. Additionally, dietary supplements offered at various retail outlets were reviewed (53), as well aspublic warnings published by the Chief Sanitary Inspectorate in Poland that addressed the risks with dietarysupplements (10).Results: The questionnaire was completed by 302 respondents (217 females, 85 males). The age of therespondents was distributed as follows: 32.45% aged 65–74, 44. % aged 75–79, 23.17% aged ≥80. Dietarysupplements consumed by seniors or widely offered in the food market mainly include ingredients asfollows: plant preparations, vitamins and minerals, electrolytes. Significantly more women have been takingdietary supplements (72%), compared to men (36%). Simultaneous administration of two or more productswas declared by 71.9% of respondents. Unfortunately, only 21% of respondents recognized any risks ofconsuming dietary supplements.Conclusion: The use of dietary supplements among elderly people has definitely increased lately. Thequestionnaire shows that approximately 86% of respondents have consumed dietary supplements at leastonce in their life, while 14% of respondents have never used these products. The survey demonstrates thatthere is a need of the continued education of the elderly about dietary supplementation and the dangers ofuncontrolled consumption of different types of supplements.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"27 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139260578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Research among the Ukrainian (Carpatian) flora’s representatives is important for searching for new active compounds.Objective: The study aims to determine the potential of callus biomass of Delphinium elatum, Anemone nemorosa, and Pulsatilla alba to become an analog of medicinal plant raw material for the use in the pharmaceutical industry, to investigate the composition, antioxidant activity and antimicrobial activity of extracts of the callus biomass of the above-mentioned plants.Methods: The individual members of the family Ranunculaceae (Delphinium elatum L., Anemone nemorosa L., Pulsatilla alba Rchb.) were introduced into in vitro culture, and their callus induction was studied using the Folin-Ciocalteu spectrophotometric method, colorimetric analysis, antioxidant, and antimicrobial activity methods.Results: The maximum content of flavonoids and phenolic compounds was observed in 40% hydro-ethanolic extracts of callus biomass and plant raw materials of D. elatum. 90.0% and 70.0% D. elatum extracts showed significant activity against Gram-positive microorganisms. 90.0/70.0/40.0% extracts showed significant activity against Bacillus licheniformis. 70.0 % extract showed significant antifungal activity against clinical and reference strains of Candida albicans. Conclusion: Summarizing experimental results, it was proved that the callus biomass of D. elatum, A. nemorosa, and P. alba have potential as analogs of medicinal plant raw materials, both for the content of biologically active substances and biological activities.
简介:研究乌克兰(喀尔帕蒂亚)植物区系代表植物对寻找新的活性化合物具有重要意义。目的:确定白头翁、海葵和白头翁愈伤组织生物量作为类似药用植物原料用于制药工业的潜力，研究上述植物愈伤组织生物量提取物的组成、抗氧化活性和抑菌活性。方法:将毛茛科植物白头翁(Delphinium elatum L.)、海葵(Anemone nemorosa L.)、白头翁(pulsatila alba Rchb.)进行离体培养，采用Folin-Ciocalteu分光光度法、比色法、抗氧化和抗菌活性法对其愈伤组织诱导进行研究。结果:黄酮类化合物和酚类化合物的含量以40%乙醇提取物和植物原料含量最高。90.0%和70.0%荷叶提取物对革兰氏阳性微生物具有显著活性。90.0/70.0/40.0%提取物对地衣芽孢杆菌活性显著。70%提取物对白色念珠菌临床和对照菌株均有显著的抗真菌活性。结论:综合实验结果，从生物活性物质的含量和生物活性两方面证明，白桦愈伤组织生物量具有作为药用植物原料类似物的潜力。
{"title":"Obtaining and research of callus biomass of some plants of the family Ranunсulaсаe","authors":"YEHOR BAZAVLUK, ROKSOLIANA VANKO, YULIAN KONECHNYI, ROKSOLANA KONECHNA","doi":"10.5604/01.3001.0053.9652","DOIUrl":"https://doi.org/10.5604/01.3001.0053.9652","url":null,"abstract":"Introduction: Research among the Ukrainian (Carpatian) flora’s representatives is important for searching for new active compounds.Objective: The study aims to determine the potential of callus biomass of Delphinium elatum, Anemone nemorosa, and Pulsatilla alba to become an analog of medicinal plant raw material for the use in the pharmaceutical industry, to investigate the composition, antioxidant activity and antimicrobial activity of extracts of the callus biomass of the above-mentioned plants.Methods: The individual members of the family Ranunculaceae (Delphinium elatum L., Anemone nemorosa L., Pulsatilla alba Rchb.) were introduced into in vitro culture, and their callus induction was studied using the Folin-Ciocalteu spectrophotometric method, colorimetric analysis, antioxidant, and antimicrobial activity methods.Results: The maximum content of flavonoids and phenolic compounds was observed in 40% hydro-ethanolic extracts of callus biomass and plant raw materials of D. elatum. 90.0% and 70.0% D. elatum extracts showed significant activity against Gram-positive microorganisms. 90.0/70.0/40.0% extracts showed significant activity against Bacillus licheniformis. 70.0 % extract showed significant antifungal activity against clinical and reference strains of Candida albicans. Conclusion: Summarizing experimental results, it was proved that the callus biomass of D. elatum, A. nemorosa, and P. alba have potential as analogs of medicinal plant raw materials, both for the content of biologically active substances and biological activities.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135126447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.5604/01.3001.0053.9615
ANAM AMIN SHAMI, TASBEEHA MARJAN, SYED ALI RAZA, HAMID MUKHTAR, MUHAMMAD WASEEM MUMTAZ, NEELAM CHAUDHARY, MUHAMMAD TAYYAB AKHTAR
Introduction: Cannabis is a dioecious plant belonging to the family Cannabaecae.Objective: The present work was conducted to study the antimicrobial activity, antioxidant potential andGC-MS-based metabolite profiling of Cannabis.Methods: The antimicrobial activity of Cannabis extract was assessed through disc-diffusion method. Theanti-oxidant potential of CEO was checked through DPPH assay and FRAP assay. We also assessed the totalphenolic (TPC) and flavonoid content (TFC) of the extract. The phytocompounds present in the Cannabisextract were characterized by using GC-MS.Results: The antimicrobial assay revealed significant activity against Pseudomonas, Staphylococcus aureusand Klebsiella pneumoniae, whereas moderate antioxidant activity was shown by Cannabis extract, having76.54% inhibition in DPPH assay and 56.10% inhibition in FRAP assay. TPC and TFC were 26.77 mg GAE/gD.E. and 34.45 mg QE/g D.W, respectively. The chemical characterization of Cannabis extract by GC-MSshows the presence of cannabinoids, terpenes, aliphatic compounds, alkanes and sesquiterpenes.
{"title":"Evaluating the antimicrobial activity, antioxidant potential and GC-MS based chemical characterization of Cannabis sativa found in Pakistan","authors":"ANAM AMIN SHAMI, TASBEEHA MARJAN, SYED ALI RAZA, HAMID MUKHTAR, MUHAMMAD WASEEM MUMTAZ, NEELAM CHAUDHARY, MUHAMMAD TAYYAB AKHTAR","doi":"10.5604/01.3001.0053.9615","DOIUrl":"https://doi.org/10.5604/01.3001.0053.9615","url":null,"abstract":"Introduction: Cannabis is a dioecious plant belonging to the family Cannabaecae.Objective: The present work was conducted to study the antimicrobial activity, antioxidant potential andGC-MS-based metabolite profiling of Cannabis.Methods: The antimicrobial activity of Cannabis extract was assessed through disc-diffusion method. Theanti-oxidant potential of CEO was checked through DPPH assay and FRAP assay. We also assessed the totalphenolic (TPC) and flavonoid content (TFC) of the extract. The phytocompounds present in the Cannabisextract were characterized by using GC-MS.Results: The antimicrobial assay revealed significant activity against Pseudomonas, Staphylococcus aureusand Klebsiella pneumoniae, whereas moderate antioxidant activity was shown by Cannabis extract, having76.54% inhibition in DPPH assay and 56.10% inhibition in FRAP assay. TPC and TFC were 26.77 mg GAE/gD.E. and 34.45 mg QE/g D.W, respectively. The chemical characterization of Cannabis extract by GC-MSshows the presence of cannabinoids, terpenes, aliphatic compounds, alkanes and sesquiterpenes.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135085777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-15DOI: 10.5604/01.3001.0053.9651
MONIKA GRZELAK, ANDRZEJ PACHOLCZAK, KAROLINA NOWAKOWSKA
Brassinosteroids (BRs) represents a new group of phytohormones. They contribute to regulation of plants growth and development, act as regulators of plant processes as seed germination, elongation growth, photomorphogenesis and photosynthesis. They are critical in response to environmental and biotic stresses like: heat, salinity, heavy metal pollution or pathogenic microorganisms. Drought stress occurs during deficiency of water in the plant environment. Lack of water in plant medium highly affects the plant development. It comes to plant processes disorders and abnormal development of plants. Long-term drought stress may cause the death of plant. Drought stress occurs in agriculture. Hence, there is a need to protect plants against its effect. Numerous reports indicate that BRs are beneficial to plants during stress, including drought. In the following research, the role of BRs in plants and usefulness of these growth regulators during stresses, especially drought, was explained. Additionally, the previous use in agriculture was described.
{"title":"Practical aspects of brassinosteroids in drought stress protection and its usage in agriculture","authors":"MONIKA GRZELAK, ANDRZEJ PACHOLCZAK, KAROLINA NOWAKOWSKA","doi":"10.5604/01.3001.0053.9651","DOIUrl":"https://doi.org/10.5604/01.3001.0053.9651","url":null,"abstract":"Brassinosteroids (BRs) represents a new group of phytohormones. They contribute to regulation of plants growth and development, act as regulators of plant processes as seed germination, elongation growth, photomorphogenesis and photosynthesis. They are critical in response to environmental and biotic stresses like: heat, salinity, heavy metal pollution or pathogenic microorganisms. Drought stress occurs during deficiency of water in the plant environment. Lack of water in plant medium highly affects the plant development. It comes to plant processes disorders and abnormal development of plants. Long-term drought stress may cause the death of plant. Drought stress occurs in agriculture. Hence, there is a need to protect plants against its effect. Numerous reports indicate that BRs are beneficial to plants during stress, including drought. In the following research, the role of BRs in plants and usefulness of these growth regulators during stresses, especially drought, was explained. Additionally, the previous use in agriculture was described.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"141 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135487289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.5604/01.3001.0053.8860
SAMIRA AICHOUR, CHAIMA MOUFFOUK, MOHAMMED BENKHALED, HAMADA HABA
Introduction: Rhamnus alaternus L. belongs to the genus Rhamnus (Rhamnaceae). It is a medicinal plantgrowing in Algeria. This plant is used worldwide as effective pharmacological remedy. Objective: This study describes in detail the phytochemical composition and the in vitro biological activitiesof R. alaternus L. aerial parts extracts. Methods: Total contents of bioactive components were estimated and the antioxidant potential was assessedby DPPH and hydroxyl radical scavenging assays, ferric reducing antioxidant activity and total antioxidantcapacity. Additionally, the haemolytic activity was evaluated towards human red blood cells and thecytotoxicity was performed using Brine shrimp lethality test. Furthermore, the antibacterial activity wasestimated in vitro against five bacteria. Seven known secondary metabolites have been established usingdifferent spectroscopic techniques such as 1D- and 2D-NMR experiments and ESI mass spectrometry andby comparison to data reported in the literature. Results: The chemical investigation of the aerial portions’ ethyl acetate extract of R. alaternus allowed thecharacterization of three triterpenoids, two phytosterols and two flavonoids. High levels of phenols andflavonoids were estimated in both ethyl acetate (EtOAc) and n-butanol (n-BuOH) extracts. These extractsexhibit strong ferric reducing antioxidant activity and significant hydroxyl and DPPH radicals scavengingeffects. Moreover, the petroleum ether (PE) extract shows a very strong hemolytic effect with 94.03 0.21% at 50 μg/mL and induces the mortality of 78.57 0.17% of Brine Shrimp nauplii at 20 μg/mL. Furthermore,the tested crude extracts (PE, EtOAc and n-BuOH) inhibit the growth of all the bacterial strains.Conclusion: R. alaternus constitutes a natural resource of bioactive substances with potential cytotoxic,antioxidant and antibacterial effects.
{"title":"Phytochemical composition, antioxidant, cytotoxic, haemolytic and antibacterial activities of aerial parts of Rhamnus alaternus","authors":"SAMIRA AICHOUR, CHAIMA MOUFFOUK, MOHAMMED BENKHALED, HAMADA HABA","doi":"10.5604/01.3001.0053.8860","DOIUrl":"https://doi.org/10.5604/01.3001.0053.8860","url":null,"abstract":"Introduction: Rhamnus alaternus L. belongs to the genus Rhamnus (Rhamnaceae). It is a medicinal plantgrowing in Algeria. This plant is used worldwide as effective pharmacological remedy. Objective: This study describes in detail the phytochemical composition and the in vitro biological activitiesof R. alaternus L. aerial parts extracts. Methods: Total contents of bioactive components were estimated and the antioxidant potential was assessedby DPPH and hydroxyl radical scavenging assays, ferric reducing antioxidant activity and total antioxidantcapacity. Additionally, the haemolytic activity was evaluated towards human red blood cells and thecytotoxicity was performed using Brine shrimp lethality test. Furthermore, the antibacterial activity wasestimated in vitro against five bacteria. Seven known secondary metabolites have been established usingdifferent spectroscopic techniques such as 1D- and 2D-NMR experiments and ESI mass spectrometry andby comparison to data reported in the literature. Results: The chemical investigation of the aerial portions’ ethyl acetate extract of R. alaternus allowed thecharacterization of three triterpenoids, two phytosterols and two flavonoids. High levels of phenols andflavonoids were estimated in both ethyl acetate (EtOAc) and n-butanol (n-BuOH) extracts. These extractsexhibit strong ferric reducing antioxidant activity and significant hydroxyl and DPPH radicals scavengingeffects. Moreover, the petroleum ether (PE) extract shows a very strong hemolytic effect with 94.03 0.21% at 50 μg/mL and induces the mortality of 78.57 0.17% of Brine Shrimp nauplii at 20 μg/mL. Furthermore,the tested crude extracts (PE, EtOAc and n-BuOH) inhibit the growth of all the bacterial strains.Conclusion: R. alaternus constitutes a natural resource of bioactive substances with potential cytotoxic,antioxidant and antibacterial effects.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"148 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135991036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-29DOI: 10.5604/01.3001.0053.8859
ELIZA WARGALA, ALEKSANDRA CHRZANOWSKA, WIKTORIA BERNATEK-SAMORAJ, IZABELA KOT
Pumpkin (Cucurbita pepo L.) seed oil, as a non-drying oil, is a raw material used in cosmetics, foodand medical industries due to its biological and nutritional properties. It is a rich source of phytosterols,protein, polyunsaturated fatty acids and vitamins. This oil exhibits antibacterial, antioxidant and antiinflammatoryproperties. The oil is used by the cosmetics industry in anti-aging, protective and hair careproducts due to its bioactive compounds such as tocopherols, phytosterols and phenolic acids, which actas antioxidants. It also has a sunscreen effect, absorbing about 20% of harmful UVB rays.
{"title":"Pumpkin (Cucurbita pepo L.) seed oil – cosmetic, food and medical raw material","authors":"ELIZA WARGALA, ALEKSANDRA CHRZANOWSKA, WIKTORIA BERNATEK-SAMORAJ, IZABELA KOT","doi":"10.5604/01.3001.0053.8859","DOIUrl":"https://doi.org/10.5604/01.3001.0053.8859","url":null,"abstract":"Pumpkin (Cucurbita pepo L.) seed oil, as a non-drying oil, is a raw material used in cosmetics, foodand medical industries due to its biological and nutritional properties. It is a rich source of phytosterols,protein, polyunsaturated fatty acids and vitamins. This oil exhibits antibacterial, antioxidant and antiinflammatoryproperties. The oil is used by the cosmetics industry in anti-aging, protective and hair careproducts due to its bioactive compounds such as tocopherols, phytosterols and phenolic acids, which actas antioxidants. It also has a sunscreen effect, absorbing about 20% of harmful UVB rays.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"391 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136283211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-29DOI: 10.5604/01.3001.0053.8858
JOANNA GRZEŚKOWIAK, DANIEL FAJFER
Introduction: The Polish cultivar of white mulberry (Morus alba L.) “Żółwińska wielkolistna” is a sourceof many bioactive substances with a wide spectrum of activities. The leaves of the plant are consideredto be a raw material richest in bioactive substances. The unique properties of the plant are primarily dueto polyphenols, in particular flavonoids, including: rutin, albanon, kuwanone, which make it possible touse white mulberry as an agent with a wide spectrum of activities, from anti-inflammatory, diaphoretic,antioxidant to antimicrobial. Objective: The aim of the study was to determine the effect of the method of extracting substances fromdried white mulberry leaves on the antibacterial activity of the obtained extracts. Four extraction methodswere used to obtain the extracts, and then their antimicrobial activity against selected Gram-positive andGram-negative bacteria was determined. Methods: Mulberry leaves were collected in September 2021 from an ecological plantation located at theExperimental Station, Institute of Natural Fibers and Medicinal Plants in Poznań – National ResearchInstitute in Pętkowo. Leaves were dried at 60C for 12 hours, then laboratory analyses were performed.The extraction was carried out by the following methods: 1.) carbon dioxide extraction, 2.) carbon dioxideextraction using a cosolvent, 3.) maceration with carbon dioxide extraction using a cosolvent, 4.) solventextraction. Then, the antimicrobial activity of the obtained extracts against selected strains of Grampositiveand Gram-negative bacteria was determined. MIC (minimum inhibitory concentration) and MBC(minimum bactericidal concentration) were determined. Results: As a result of the conducted research it was found that there are differences in the antibacterialactivites of extracts from dried white mulberry leaves depending on the extraction method used. Thehighest antibacterial activity both against Gram-positive and Gram-negative bacteria was shown by extractsobtained using a supercritical carbon dioxide extractor without the use of cosolventa, with more effectivegrowth-inhibiting and bactericidal effects observed against Gram-positive bacteria compared to Gramnegativebacteria. Conclusions: Extracts obtained from dried leaves of white mulberry (Morus alba L.) of the Polish variety“Żółwińska wielkolistna” have antibacterial properties. This leads to the possibility of using white mulberryin herbal medicine and pharmacology as an antimicrobial agent.
简介:波兰栽培的白桑树(Morus alba L.)“Żółwińska wielkolistna”是许多生物活性物质的来源，具有广泛的活性。植物的叶子被认为是生物活性物质最丰富的原料。该植物的独特特性主要是由于多酚，特别是黄酮类化合物，包括:芦丁，阿尔巴尼亚，库瓦酮，这使得它有可能使用白桑作为一种具有广泛活性的药剂，从抗炎，发汗，抗氧化到抗菌。目的:研究不同提取方法对白桑叶提取物抑菌活性的影响。采用4种提取方法获得提取物，并测定其对选定革兰氏阳性菌和革兰氏阴性菌的抑菌活性。方法:桑叶采集于2021年9月，采集地点为位于Pętkowo的波兹纳齐国家研究所天然纤维和药用植物研究所实验站的一个生态人工林。在60℃下干燥12小时，然后进行实验室分析。提取方法为:1.二氧化碳萃取法，2.共溶剂二氧化碳萃取法，3.共溶剂二氧化碳浸渍萃取法，4.溶剂萃取法。然后测定所得提取物对革兰氏阳性菌和革兰氏阴性菌的抑菌活性。测定最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果:根据提取方法的不同，白桑叶提取物的抑菌活性存在差异。对革兰氏阳性菌和革兰氏阴性菌的抑菌活性最高的是使用超临界二氧化碳萃取器而不使用co溶剂提取的提取物，与革兰氏阴性菌相比，对革兰氏阳性菌具有更有效的生长抑制和杀菌作用。结论:从波兰品种“Żółwińska wielkolistna”的白桑(Morus alba L.)干叶中提取的提取物具有抗菌性能。这就有可能在草药和药理学中使用白桑作为抗菌剂。
{"title":"The effect of the extraction method on the antibacterial activity of white mulberry extracts","authors":"JOANNA GRZEŚKOWIAK, DANIEL FAJFER","doi":"10.5604/01.3001.0053.8858","DOIUrl":"https://doi.org/10.5604/01.3001.0053.8858","url":null,"abstract":"Introduction: The Polish cultivar of white mulberry (Morus alba L.) “Żółwińska wielkolistna” is a sourceof many bioactive substances with a wide spectrum of activities. The leaves of the plant are consideredto be a raw material richest in bioactive substances. The unique properties of the plant are primarily dueto polyphenols, in particular flavonoids, including: rutin, albanon, kuwanone, which make it possible touse white mulberry as an agent with a wide spectrum of activities, from anti-inflammatory, diaphoretic,antioxidant to antimicrobial. Objective: The aim of the study was to determine the effect of the method of extracting substances fromdried white mulberry leaves on the antibacterial activity of the obtained extracts. Four extraction methodswere used to obtain the extracts, and then their antimicrobial activity against selected Gram-positive andGram-negative bacteria was determined. Methods: Mulberry leaves were collected in September 2021 from an ecological plantation located at theExperimental Station, Institute of Natural Fibers and Medicinal Plants in Poznań – National ResearchInstitute in Pętkowo. Leaves were dried at 60C for 12 hours, then laboratory analyses were performed.The extraction was carried out by the following methods: 1.) carbon dioxide extraction, 2.) carbon dioxideextraction using a cosolvent, 3.) maceration with carbon dioxide extraction using a cosolvent, 4.) solventextraction. Then, the antimicrobial activity of the obtained extracts against selected strains of Grampositiveand Gram-negative bacteria was determined. MIC (minimum inhibitory concentration) and MBC(minimum bactericidal concentration) were determined. Results: As a result of the conducted research it was found that there are differences in the antibacterialactivites of extracts from dried white mulberry leaves depending on the extraction method used. Thehighest antibacterial activity both against Gram-positive and Gram-negative bacteria was shown by extractsobtained using a supercritical carbon dioxide extractor without the use of cosolventa, with more effectivegrowth-inhibiting and bactericidal effects observed against Gram-positive bacteria compared to Gramnegativebacteria. Conclusions: Extracts obtained from dried leaves of white mulberry (Morus alba L.) of the Polish variety“Żółwińska wielkolistna” have antibacterial properties. This leads to the possibility of using white mulberryin herbal medicine and pharmacology as an antimicrobial agent.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136283210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.5604/01.3001.0053.6136
Shagun Sharma, Harcharan SINGH DHALIWAL, V. Sharma
The genus Gentiana (L.) belongs to the family Gentianaceae. In the present cytomorphological study, no new cytotype was reported in the selected species, namely G. kurroo Royle, G. moorcroftiana Wall. ex G. Don, G. pedicellata D. Don. Grisb. Further, morphometric characters also aligned with the previous results. Whereas, phytochemical screening through preliminary total phenolic content analysis revealed that all three species collected from Keylong (Lahaul & Spiti) had more prominent results than species collected from two other locations of Himachal Pradesh. Thereafter, further phytochemical and bioactivities studies were focused only on the samples/species collected from Keylong (Lahaul & Spiti).
{"title":"Botanical and phyto-pharmacological exploration of genus Gentiana (L.) from Himachal Pradesh, critically endangered medicinal plant","authors":"Shagun Sharma, Harcharan SINGH DHALIWAL, V. Sharma","doi":"10.5604/01.3001.0053.6136","DOIUrl":"https://doi.org/10.5604/01.3001.0053.6136","url":null,"abstract":"The genus Gentiana (L.) belongs to the family Gentianaceae. In the present cytomorphological study, no new cytotype was reported in the selected species, namely G. kurroo Royle, G. moorcroftiana Wall. ex G. Don, G. pedicellata D. Don. Grisb. Further, morphometric characters also aligned with the previous results. Whereas, phytochemical screening through preliminary total phenolic content analysis revealed that all three species collected from Keylong (Lahaul & Spiti) had more prominent results than species collected from two other locations of Himachal Pradesh. Thereafter, further phytochemical and bioactivities studies were focused only on the samples/species collected from Keylong (Lahaul & Spiti).","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43813553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.5604/01.3001.0053.7211
Filip Przerwa, A. Janiec, Jakub Byrski, Justyna Aleksandrzak, Mateusz Gutowski, Karol Adamczyk, K. Kotrych, I. Uzar
Cancer is one of the most serious problems facing modern medicine. Research on new methods of treating this disease is being conducted. Existing methods are increasingly effective, but they also have risks to human health. The attention of scientists is focused on compounds of natural origin, as they are less toxic to human body than traditional chemotherapeutics. They are also much more accessible and their production is much less complex and expensive. There are several natural compounds with anticancer potential. In this article, we present three groups of potential therapeutic importance - polyphenols, brassinosteroids, and flavonoids.
{"title":"Selected substances of natural origin with anticancer activity","authors":"Filip Przerwa, A. Janiec, Jakub Byrski, Justyna Aleksandrzak, Mateusz Gutowski, Karol Adamczyk, K. Kotrych, I. Uzar","doi":"10.5604/01.3001.0053.7211","DOIUrl":"https://doi.org/10.5604/01.3001.0053.7211","url":null,"abstract":"Cancer is one of the most serious problems facing modern medicine. Research on new methods of treating this disease is being conducted. Existing methods are increasingly effective, but they also have risks to human health. The attention of scientists is focused on compounds of natural origin, as they are less toxic to human body than traditional chemotherapeutics. They are also much more accessible and their production is much less complex and expensive. There are several natural compounds with anticancer potential. In this article, we present three groups of potential therapeutic importance - polyphenols, brassinosteroids, and flavonoids.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47815217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.5604/01.3001.0053.6137
A. Fulga, S. Protopop, V. Gudumac, Mihail Todiras, O. Tagadiuc
Taraxacum officinale (TO), commonly known as dandelion, has demonstrated its effectiveness against many types of tumours, like hepatocellular and colon carcinomas, melanoma, leukaemia.
{"title":"Dandelion suppresses glioblastoma another assessment with the same result","authors":"A. Fulga, S. Protopop, V. Gudumac, Mihail Todiras, O. Tagadiuc","doi":"10.5604/01.3001.0053.6137","DOIUrl":"https://doi.org/10.5604/01.3001.0053.6137","url":null,"abstract":"Taraxacum officinale (TO), commonly known as dandelion, has demonstrated its effectiveness against many types of tumours, like hepatocellular and colon carcinomas, melanoma, leukaemia.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46771078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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