Bioink based on the dECM for 3D bioprinting of bionic tissue, the first results obtained on murine model

Q1 Computer Science Bioprinting Pub Date : 2022-12-01 DOI:10.1016/j.bprint.2022.e00233

Marta Klak , Katarzyna Kosowska , Tomasz Bryniarski , Ilona Łojszczyk , Tomasz Dobrzański , Grzegorz Tymicki , Anna Filip , Andrzej Antoni Szczepankiewicz , Radosław Olkowski , Anna Kosowska , Andrzej Berman , Artur Kamiński , Michał Wszoła

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引用次数: 3

Abstract

Tissue engineering is an intensively developing field of modern medicine. However, to transfer the research from the laboratory scale to the clinic, it is necessary to produce biocompatible bioinks, that will be safe for both patients and cells used in the bioprinting process itself.

The aim of this researches was to produce a bioink that would enable bioprinting of structures with biological material, improving their functionality by recreating their natural environment.

The results showed the appropriate physicochemical properties of the biomaterial, which is stable in vitro and in vivo. In vitro studies showed the highest functionality of islets (7 days) in the bioprinted cell-printed constructs. The cytotoxicity tests showed the cell viability at the level of 120%. Moreover, the paper presents the first in vivo results for biodegradation of bionic cell-printed constructs. During these studies, no inflammatory reaction to the implanted cell-printed construct was found for 12 months. The appearance of the first neovascular processes was shown in the 8th week of the experiment. At that time, there were no deviations in the level of blood parameters: AST,ALT,KC, IL-6 and TNF-α.

Summing up, the created bioink composition is safe for living organisms and contributes to the improvement of the functionality of pancreatic islets.

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基于dECM的Bioink用于仿生组织的3D生物打印，这是在小鼠模型上获得的第一个结果

组织工程是现代医学中一个正在蓬勃发展的领域。然而，为了将研究从实验室规模转移到临床，有必要生产出生物相容性的生物墨水，这将对患者和用于生物打印过程本身的细胞都是安全的。这项研究的目的是生产一种生物链接，使生物材料结构的生物打印成为可能，通过重建其自然环境来提高其功能。实验结果表明，该生物材料具有良好的理化性质，在体内和体外均稳定。体外研究表明，在生物打印的细胞打印结构中，胰岛的功能最高(7天)。细胞毒性试验显示细胞存活率为120%。此外，本文还首次提出了仿生细胞打印结构生物降解的体内结果。在这些研究中，植入的细胞打印构建体在12个月内没有发现炎症反应。第一个新生血管突出现在实验的第8周。同期血液参数AST、ALT、KC、IL-6、TNF-α均无差异。综上所述，所制备的生物链接物对生物体是安全的，并有助于改善胰岛的功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioprinting Computer Science-Computer Science Applications

CiteScore

11.50

自引率

0.00%

发文量

审稿时长

68 days

期刊介绍： Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.