Optimization of chromosome doubling treatment for efficient in vivo doubled haploid production in maize

IF 0.9 4区 生物学 Q4 PLANT SCIENCES Israel Journal of Plant Sciences Pub Date : 2021-01-27 DOI:10.1163/22238980-BJA10020
Sourbh Kumar, U. Chandel, S. Guleria
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引用次数: 2

Abstract

An investigation to optimize the protocol for application of colchicine for enhancing the doubled haploid production in maize was done. 106 maize genotypes were used as maternal parents, whereas, pollen source involved tropically adopted haploid inducer (TAIL P1 and TAIL hybrid). After the elimination of chromosomes of inducer lines, haploid seeds were obtained from the crosses. Haploid seedlings were treated with three different doses, such as 0.04, 0.06 and 0.08 per cent of colchicines for different durations (8, 12 and 15 hours). The response of various colchicine concentrations applied for different time durations revealed significant differences at P ≤ 0.05 for various parameters viz., per cent plants survivability, stalk colour, the fertility of tassel, silk present/absent, pollen viability, seed set and per cent doubled haploid formation. In maize, colchicine doses of 0.04 per cent for 12 hours and 0.06 per cent for 8 hours, respectively were established as optimum for enhanced doubled haploid production. But among these two, 0.04 per cent for 12 hours was observed to be best dose for doubled haploid production in maize.
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玉米体内高效双单倍体的染色体加倍处理优化
研究了秋水仙碱用于提高玉米单倍体加倍产量的优化方案。106个玉米基因型被用作母本,而花粉源涉及热带采用的单倍体诱导物(TAIL P1和TAIL杂交种)。在消除诱导系的染色体后,从杂交中获得单倍体种子。用三种不同剂量处理单倍体幼苗,如0.04、0.06和0.08%的秋水仙碱,持续不同的时间(8、12和15小时)。不同浓度秋水仙碱对不同持续时间的响应显示,在P≤0.05时,不同参数存在显著差异,即植物存活率、茎色、流苏育性、有丝/无丝、花粉活力、结实率和单倍体形成率。在玉米中,秋水仙碱剂量分别为0.04%,持续12小时和0.06%,持续8小时,被确定为提高加倍单倍体产量的最佳剂量。但在这两者中,0.04%的剂量持续12小时是玉米单倍体产量翻倍的最佳剂量。
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来源期刊
Israel Journal of Plant Sciences
Israel Journal of Plant Sciences 生物-植物科学
CiteScore
1.90
自引率
0.00%
发文量
17
审稿时长
>12 weeks
期刊介绍: The Israel Journal of Plant Sciences is an international journal of extensive scope that publishes special issues dealing with all aspects of plant sciences, including but not limited to: physiology, cell biology, development, botany, genetic
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