Aggregation of a parthenogenetic diploid embryo and a male embryo improves the blastocyst development and parthenogenetic embryonic stem cell derivation

IF 1.1 4区 生物学 Q3 BIOLOGY Turkish Journal of Biology Pub Date : 2017-08-01 DOI:10.3906/BIY-1612-30
Xiaoyan Qiu, Nan Li, Xiong Xiao, Yuemin Li
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引用次数: 6

Abstract

Parthenogenetically derived mammalian embryos, with no paternal genome, are not viable and die, largely from defective placental growth attributed to a lack of paternal effect, resulting in the low blastulation rate and low derivation efficiency of parthenogenetic embryonic stem cells (pESCs). Therefore, the present study, by the optimization of parthenogenetic embryo production and the aggregation of the parthenogenetic diploid embryo and the identified male embryo, aims to investigate a method to improve the development of parthenogenetic embryo and pESC derivation in mice. Using different chemical combinations for the optimization, we found that the heterozygous diploid type had a significantly higher blastulation rate than the haploid type (P < 0.05). The treatment of strontium chloride (SrCl2) combined with cytochalasin B for 4 h produced the highest heterozygous diploid rate and blastulation rate. Our self-made concave hole system was used for the aggregation of the parthenogenetic heterozygous diploid embryo with the male embryo identified by the duplex PCR method, and we found that the chimeric embryo had an improved rate of blastulation and pESC isolation.
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孤雌生殖二倍体胚胎和雄性胚胎的聚集改善了胚泡发育和孤雌生殖胚胎干细胞的衍生
没有父系基因组的孤雌生殖来源的哺乳动物胚胎不能存活并死亡,主要是由于缺乏父系影响导致胎盘生长缺陷,导致孤雌生殖胚胎干细胞(pESCs)的囊胚率低,衍生效率低。因此,本研究旨在通过优化单性生殖胚胎的生产,将单性生殖二倍体胚胎与鉴定的雄性胚胎聚集在一起,探索一种改善小鼠单性生殖胚胎发育和pESC衍生的方法。通过不同的化学组合进行优化,我们发现杂合二倍体型的胚泡率显著高于单倍体型(P < 0.05)。氯化锶(SrCl2)与细胞松弛素B联合处理4 h,产生最高的杂合二倍体率和囊胚率。我们自制的凹孔系统将孤雌杂合二倍体胚与双链PCR鉴定的雄性胚聚集,发现嵌合胚的成胚率和pESC分离率均有提高。
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来源期刊
CiteScore
4.60
自引率
0.00%
发文量
20
审稿时长
6-12 weeks
期刊介绍: The Turkish Journal of Biology is published electronically 6 times a year by the Scientific and Technological Research Council of Turkey (TÜBİTAK) and accepts English-language manuscripts concerning all kinds of biological processes including biochemistry and biosynthesis, physiology and metabolism, molecular genetics, molecular biology, genomics, proteomics, molecular farming, biotechnology/genetic transformation, nanobiotechnology, bioinformatics and systems biology, cell and developmental biology, stem cell biology, and reproductive biology. Contribution is open to researchers of all nationalities.
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