{"title":"Issue Information","authors":"","doi":"10.1002/cpph.64","DOIUrl":null,"url":null,"abstract":"<p><b>Cover</b>: In Caine et al. (http://doi.org/10.1002/cpph.81), the image shows schematic of HaloPROTAC3 degradation of HaloTag protein fusions in live cells. First, CRISPR/Cas9 technology is used to insert HaloTag or HiBiT-HaloTag into the genomic locus of the target protein using a dsDNA donor plasmid and Cas9-crRNA complex (1). After the HaloTag fusion is expressed, cells are treated with HaloPROTAC3(2). HaloPROTAC3 induces a ternary complex between the VHL E3 ligase component and HaloTag protein fusion, resulting degradation of the HaloTag target protein via the ubiquitin-proteasomal pathway (3).\n\n <figure>\n <div><picture>\n <source></source></picture><p></p>\n </div>\n </figure></p>","PeriodicalId":10871,"journal":{"name":"Current Protocols in Pharmacology","volume":"91 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpph.64","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Pharmacology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpph.64","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}
引用次数: 0
Abstract
Cover: In Caine et al. (http://doi.org/10.1002/cpph.81), the image shows schematic of HaloPROTAC3 degradation of HaloTag protein fusions in live cells. First, CRISPR/Cas9 technology is used to insert HaloTag or HiBiT-HaloTag into the genomic locus of the target protein using a dsDNA donor plasmid and Cas9-crRNA complex (1). After the HaloTag fusion is expressed, cells are treated with HaloPROTAC3(2). HaloPROTAC3 induces a ternary complex between the VHL E3 ligase component and HaloTag protein fusion, resulting degradation of the HaloTag target protein via the ubiquitin-proteasomal pathway (3).
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