Silver nanoparticles induced genotoxicity and oxidative stress in tomato plants

IF 1.1 4区 生物学 Q3 BIOLOGY Turkish Journal of Biology Pub Date : 2017-10-01 DOI:10.3906/BIY-1608-36
F. Çekiç, Sefa Ekinci, Muslum S. Inal, D. Ozakca
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引用次数: 34

Abstract

Among nanoparticles, silver nanoparticles (AgNPs) are intensively used in many materials owing to their antibacterial effects. In the present study different concentrations of AgNPs in Hoagland solution were applied to tomato seedlings. Total chlorophyll content, relative water content (RWC), antioxidant enzyme activities, and malondialdehyde content (MDA) as well as the genomic template stability (GTS) were analyzed. The intersimple sequence repeat polymerase chain reaction assay (ISSR-PCR) was used to determine the genotoxic effects of AgNPs on DNA. RWC did not change under AgNPs treatments; however, total chlorophyll content was significantly reduced by AgNPs applications. ISSR profiles demonstrated a consistent increase in polymorphic bands by the increase in the concentration of AgNPs. GTS value was also reduced depending on the concentration of AgNPs. SOD and APX activities were increased under low AgNPs treatments; however, these activities were decreased under high concentrations of AgNPs treatments. Tomato plants could be sensitive to AgNPs within the increase in MDA content in all of the AgNPs treatments. AgNPs nanotoxicity could be quite dose-dependent. AgNPs could also have negative effects on tomato plants by enhancing DNA damage and lipid peroxidation.
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银纳米粒子诱导番茄植株的遗传毒性和氧化应激
在纳米颗粒中,银纳米颗粒由于其抗菌作用而被广泛应用于许多材料中。在本研究中,将Hoagland溶液中不同浓度的AgNPs应用于番茄幼苗。分析了总叶绿素含量、相对含水量(RWC)、抗氧化酶活性、丙二醛含量(MDA)以及基因组模板稳定性(GTS)。单序列间重复聚合酶链式反应(ISSR-PCR)用于测定AgNPs对DNA的遗传毒性作用。在AgNPs处理下RWC没有变化;然而,施用AgNPs显著降低了总叶绿素含量。ISSR图谱显示,随着AgNPs浓度的增加,多态性条带持续增加。GTS值也根据AgNPs的浓度而降低。在低AgNPs处理下,SOD和APX活性增加;然而,在高浓度的AgNPs处理下,这些活性降低。在所有AgNPs处理中,番茄植株对MDA含量的增加可能对AgNPs敏感。AgNPs的纳米毒性可能具有相当的剂量依赖性。AgNPs也可能通过增强DNA损伤和脂质过氧化对番茄植株产生负面影响。
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来源期刊
CiteScore
4.60
自引率
0.00%
发文量
20
审稿时长
6-12 weeks
期刊介绍: The Turkish Journal of Biology is published electronically 6 times a year by the Scientific and Technological Research Council of Turkey (TÜBİTAK) and accepts English-language manuscripts concerning all kinds of biological processes including biochemistry and biosynthesis, physiology and metabolism, molecular genetics, molecular biology, genomics, proteomics, molecular farming, biotechnology/genetic transformation, nanobiotechnology, bioinformatics and systems biology, cell and developmental biology, stem cell biology, and reproductive biology. Contribution is open to researchers of all nationalities.
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