In Vitro Probiotic Characteristics of the Lactococcus Lactis Subsp. Hordniae Strain UAB10 Isolated from Aloe Vera

IF 0.4 4区医学 Q4 NUTRITION & DIETETICS Current Topics in Nutraceutical Research Pub Date : 2023-01-06 DOI:10.37290/ctnr2641-452x.21:60-65

Hyung-Jin Lim, Yeong‐Seon Won, S. Bak, Soyoung Lee, Bomi Ryu, Seon-Heui Cha, Taeoh Kwon, K. Shim, Jisu Jeon, Hyuck Se Kwon, S. Lee, Seung-Jae Lee, M. Rho

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Abstract

We have isolated 15 bacterial strains from Aloe vera gel. Among them, we selected the Lactococcus lactis subsp. hordniae strain UAB10 based on its inhibition of IL-6-induced STAT3 activation and its ability to adhere to intestinal epithelial cells. UAB10 showed strong inhibition of interleukin-6-induced signal transducer and activator of transcription 3 activation. The UAB10 isolate showed superior adhesion to Caco-2 cells compared with KTCT 3768, a reference L. lactis subsp. hordniae strain. The UAB10 isolate displayed no hemolytic activity and more potent anti-Shigella flexneri activity than KTCT 3768. Furthermore, UAB10 was able to survive better than KTCT 3768 in oxgall-containing De Man, Rogosa, and Sharpe agar media and low-pH De Man, Rogosa, and Sharpe agar media. Finally, UAB10 did not show any resistance against the eight tested antibiotics, including ampicillin, vancomycin, and gentamicin. These results highlight the potential for the use of UAB10 as a commercial probiotic.

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乳酸乳球菌亚群的体外益生菌特性从芦荟中分离的Hordniae菌株UAB10

我们从芦荟凝胶中分离出15种细菌菌株。其中，我们选取了乳酸乳球菌亚种。基于其抑制il -6诱导的STAT3激活及其粘附肠上皮细胞的能力。UAB10对白细胞介素-6诱导的信号转导因子和转录3激活因子有较强的抑制作用。与乳酸菌对照亚种KTCT 3768相比，UAB10分离物对Caco-2细胞的粘附能力更强。hordniae压力。与KTCT 3768相比，UAB10分离物无溶血活性，抗福氏志贺氏菌活性更强。此外，UAB10在含氧的De Man、Rogosa和Sharpe琼脂培养基和低ph的De Man、Rogosa和Sharpe琼脂培养基上的存活率优于KTCT 3768。最后，UAB10对包括氨苄西林、万古霉素和庆大霉素在内的八种测试抗生素没有表现出任何耐药性。这些结果突出了UAB10作为一种商业益生菌的潜力。

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来源期刊

Current Topics in Nutraceutical Research 医学-药学

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Current Topics in Nutraceutical Research is an international, interdisciplinary broad-based peer reviewed scientific journal for critical evaluation of research on chemistry, biology and therapeutic applications of nutraceuticals and functional foods. The major goal of this journal is to provide peer reviewed unbiased scientific data to the decision makers in the nutraceutical and food industry to help make informed choices about development of new products. To this end, the journal will publish two types of review articles. First, a review of preclinical research data coming largely from animal, cell culture and other experimental models. Such data will provide basis for future product development and/or human research initiatives. Second, a critical evaluation of current human experimental data to help market and deliver the product for medically proven use. This journal will also serve as a forum for nutritionists, internists, neurologists, psychiatrists, and all those interested in preventive medicine. The common denominator of all of the topic to be covered by the journal must include nutraceuticals and/functional food. The following is an example of some specific areas that may be of interest to the journal. i) Role of vitamins, minerals, antioxidants and phytonutrients on cardiovascular health, cancer, diabetes, ocular health, mental health, men’s health, women’s health, infant nutrition, ii) Role of herbals on human health, iii) Dietary supplements and sleep, iv) Components of diet that may have beneficial effect on human health, v) regulation of apoptosis and cell viability, vi) Isolation and characterization of bioactive components from functional foods, vii) Nutritional genomics, and viii) Nutritional proteomics.