Temperature and pH Optimization for Protease Production Fermented by Yarrowia lipolytica from Agro-Industrial Waste

IF 3.3 3区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Fermentation-Basel Pub Date : 2023-09-07 DOI:10.3390/fermentation9090819
J. López-Trujillo, Miguel Mellado-Bosque, J. Ascacio-Valdés, L. Prado-Barragán, J. A. Hernández-Herrera, A. Aguilera-Carbó
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引用次数: 1

Abstract

The use of yeasts for the production of proteases has increased in demand in recent years. Y. lipolytica has been reported as a strain with high yields of protease production. This work aimed to evaluate the impact of pH and temperature on the production of proteases using Y. lipolytica in solid-state fermentation (SSF). Soybean, canola meal, cottonseed meal, and sesame meal wastes were used as nutrient sources at seven pH levels (4, 5, 6, 7, 8, 9, 10) and five temperatures (25, 30, 35, 40, 45 °C). The waste source and optimal conditions for maximum enzyme production (EP) were obtained by Box–Benhken design. The results revealed that at pH of 7, temperature of 30 °C, and for 48 h cultivation period, canola meal showed the best EP with 188.75 U/L, followed by soybean with 117.07 U/L, cottonseed meal with 66.71 U/L, and sesame with the lowest production, reaching 88.5 U/L up to 35 °C. The temperature factor exhibited the greatest effect on protease production. The biotechnological and economic potential of canola meal in the production of enzymes is highlighted.
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解脂Yarrowia从农业工业废弃物中发酵蛋白酶的温度和pH优化
近年来,利用酵母生产蛋白酶的需求有所增加。据报道,解脂芽孢杆菌是一种高产蛋白酶菌株。本研究旨在评估pH和温度对固态发酵(SSF)中解脂y菌生产蛋白酶的影响。在7种pH值(4、5、6、7、8、9、10)和5种温度(25、30、35、40、45℃)条件下,利用大豆、菜籽粕、棉籽粕和芝麻粕废弃物作为营养源。通过Box-Benhken设计,确定了产酶量的最佳条件。结果表明,在pH = 7、温度为30℃、培养时间为48 h的条件下,油菜籽粕的EP最高,为188.75 U/L,其次是大豆,为117.07 U/L,棉籽粕为66.71 U/L,芝麻最低,35℃时产量为88.5 U/L。温度因子对蛋白酶产生的影响最大。强调了菜籽粕在酶生产中的生物技术和经济潜力。
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来源期刊
Fermentation-Basel
Fermentation-Basel BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
3.80
自引率
18.90%
发文量
594
审稿时长
7 weeks
期刊介绍:
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