In vitro anti-inflammatory activity of Pachymelania aurita (Muller) and Tympanotonos fuscatus var. radula (Linnaeus) whole body extracts in lipopolysaccharide-stimulated peritoneal macrophage cells

Q3 Agricultural and Biological Sciences Biological Letters Pub Date : 2022-04-22 DOI:10.2478/biolet-2021-0001
Queensley Eghianruwa, O. Osoniyi, N. Maina, S. Wachira, Regina W. Mbugua, M. Imbuga
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Abstract

Abstract Inflammation is the immune system’s response to infection and injury. Although it is a natural and beneficial process, it has been implicated in the pathogeneses of arthritis, cancer, neurodegenerative, and cardiovascular disease. In this study, we evaluated the in vitro anti-inflammatory activity of the alcohol and aqueous extracts of marine gastropods Pachymelania aurita and Tympanotonos fuscatus (syn. Tympanotonus fuscatus) var. radula in peritoneal macrophage cells treated with lipopolysaccharide. Peripheral macrophages were harvested from mice following the injection of foetal calf serum into the peritoneal space. The cytotoxicity of the extracts on the macrophages and the maximum non-toxic dose was determined using the MTT assay. Cells were challenged with lipopolysaccharide and treated with crude extracts of T. fuscatus and P. aurita 24 h later. The antioxidant activity was also evaluated, using the DPPH assay. Both extracts of P. aurita and T. fuscatus exhibited significant anti-inflammatory activities in terms of their ability to inhibit the production of NO, TNF-α, IL-1 and PGE2, which are key mediators of the inflammation response. Also, the alcohol extracts proved to be as effective as vitamin C in the free radical scavenging of DPPH. Thus, the extracts from P. aurita and T. fuscatus are useful candidates for further drug studies, especially as drug candidates for the treatment of inflammatory conditions.
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耳厚黑菌(Muller)和褐鼓鼓菌(Linnaeus)全身提取物在脂多糖刺激的腹腔巨噬细胞中的体外抗炎活性
摘要炎症是免疫系统对感染和损伤的反应。尽管它是一个自然和有益的过程,但它与关节炎、癌症、神经退行性疾病和心血管疾病的病因有关。在本研究中,我们评估了海洋腹足类Pachymelania aurita和Tympanotonos fuscatus(syn.Tymampanonus fuscattus)var.radula的酒精和水提取物在脂多糖处理的腹膜巨噬细胞中的体外抗炎活性。在将胎牛血清注射到腹膜空间中之后,从小鼠收获外周巨噬细胞。用MTT法测定提取物对巨噬细胞的细胞毒性和最大无毒剂量。用脂多糖攻击细胞,并在24小时后用褐藻和金黄色葡萄的粗提取物处理。还使用DPPH测定法评估了抗氧化活性。金黄色葡萄和褐藻的提取物都表现出显著的抗炎活性,因为它们能够抑制NO、TNF-α、IL-1和PGE2的产生,而这些是炎症反应的关键介质。此外,在清除DPPH的自由基方面,醇提取物被证明与维生素C一样有效。因此,金黄色葡萄球菌和灰褐色葡萄球菌的提取物是进一步药物研究的有用候选者,特别是作为治疗炎症条件的候选药物。
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Biological Letters
Biological Letters Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
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期刊介绍: It covers a wide spectrum of biological disciplines, including animal science and zoology, general biochemistry, botany, and ecology. We also encourage submission of manuscripts related to conservation biology and wildlife management.
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