Lack of salidroside impact on selected cytochromes encoding genes transcription in the liver of ethanol induced rats

Q3 Medicine Herba Polonica Pub Date : 2021-09-01 DOI:10.2478/hepo-2021-0016

Radosław Kujawski, M. Szulc, Maria Toboła, M. Graczyk, Kamila Czora-Poczwardowska, Justyna Baraniak, Małgorzata Kania-Dobrowolska, Julia Słyńko-Krzyżostaniak, A. Krajewska-Patan, P. Mikołajczak

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Abstract

Summary Introduction: The molecular basis of in vivo metabolism of selected representatives of phenylethanoids in the presence of ethanol has not been fully elucidated. Objective: The aim was to estimate a salidroside (Sal) metabolism in the liver tissue in rats with induced alcohol tolerance by assessing changes in the transcription of genes encoding cytochromes: CYP1A2, 2D2, 3A1, 2C23. Methods: cDNA was synthesized from total RNA isolated from rat liver samples. mRNA level changes were evaluated using real-time PCR (qRT-PCR) technique. Results: Ethanol caused a significant induction of the CYP1A2 and CYP2C23 genes transcription, and a decrease in the CYP3A1 mRNA level, predominantly without statistical significance. A statistically significant increase of the CYP1A2 mRNA level was observed in the group receiving only Sal (4.5 mg/kg b.w.; p.o.) (p<0.01). Conclusions: There was no unequivocal effect of salidroside on the transcription of investigated cytochrome genes in the liver of rats with induced alcohol tolerance.

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红景天苷缺乏对乙醇诱导大鼠肝脏中选定的细胞色素编码基因转录的影响

摘要简介:苯乙醇在乙醇存在下的体内代谢的分子基础尚未完全阐明。目的:通过评估编码细胞色素CYP1A2、2D2、3A1、2C23基因的转录变化，评估诱导酒精耐受大鼠肝组织中红柳苷(Sal)的代谢。方法:从大鼠肝脏中分离总RNA合成cDNA。采用实时荧光定量PCR (qRT-PCR)技术检测mRNA水平变化。结果:乙醇显著诱导CYP1A2和CYP2C23基因转录，CYP3A1 mRNA水平降低，但以无统计学意义为主。CYP1A2 mRNA水平在仅接受Sal (4.5 mg/kg b.w;订单。)(p < 0.01)。结论:红景天苷对酒精耐受大鼠肝脏细胞色素基因的转录没有明确的影响。

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