S. Chandra , P. Halder , M. Kumar , K. Mukhopadhyay
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引用次数: 2
Abstract
SNAREs (soluble N-ethylmaleimide sensitive factor adaptor protein receptors) are small polypeptides characterized by a particular domain called the SNARE motif. Compared with the genome of other eukaryotes, monocotyledonous and dicotyledonous plants have more SNAREs indicating their important roles in higher plant species. Higher plants have the capability to form SNARE complexes that are important in determining the precise process of vesicle fusion for intracellular trafficking pathways. SNAREs have been reported to be engaged in the delivery of cell wall precursors to the newly formed cell plate during cytokinesis. The role of SNARE genes in response to plant-pathogen interaction is still not well understood. We found 35 SNARE genes in the wheat genome using a Hidden Markov Model. In this study with combined usage of in silico and molecular cloning technologies, we identified and characterized three SNARE genes (SNARE3, SNARE5 and SNARE6). The deduced amino acid sequences of these SNARE genes contained two characteristic conserved domains – a SNARE motif and a transmembrane domain, and they showed a high degree of homology with other eukaryotic SNARE genes. Phylogenetic analysis and three dimensional structures built with the help of Modeller software confirmed the presence of SNARE motifs in the proteins. The spatio-temporal expression profiling studies exemplify the positive role of SNARE transcripts have in resistant and susceptible wheat plants during incompatible and compatible interaction respectively, in response to Puccinia triticina induced leaf-rust infection. Taken together, our study suggests a role for SNARE genes in vesicle mediated resistance to leaf rust in wheat.
Agri GeneAgricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
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期刊介绍:
Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.