Genome-wide identification, cloning and characterization of SNARE genes in bread wheat (Triticum aestivum L.) and their response to leaf rust

Q1 Agricultural and Biological Sciences Agri Gene Pub Date : 2017-03-01 DOI:10.1016/j.aggene.2016.11.002
S. Chandra , P. Halder , M. Kumar , K. Mukhopadhyay
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引用次数: 2

Abstract

SNAREs (soluble N-ethylmaleimide sensitive factor adaptor protein receptors) are small polypeptides characterized by a particular domain called the SNARE motif. Compared with the genome of other eukaryotes, monocotyledonous and dicotyledonous plants have more SNAREs indicating their important roles in higher plant species. Higher plants have the capability to form SNARE complexes that are important in determining the precise process of vesicle fusion for intracellular trafficking pathways. SNAREs have been reported to be engaged in the delivery of cell wall precursors to the newly formed cell plate during cytokinesis. The role of SNARE genes in response to plant-pathogen interaction is still not well understood. We found 35 SNARE genes in the wheat genome using a Hidden Markov Model. In this study with combined usage of in silico and molecular cloning technologies, we identified and characterized three SNARE genes (SNARE3, SNARE5 and SNARE6). The deduced amino acid sequences of these SNARE genes contained two characteristic conserved domains – a SNARE motif and a transmembrane domain, and they showed a high degree of homology with other eukaryotic SNARE genes. Phylogenetic analysis and three dimensional structures built with the help of Modeller software confirmed the presence of SNARE motifs in the proteins. The spatio-temporal expression profiling studies exemplify the positive role of SNARE transcripts have in resistant and susceptible wheat plants during incompatible and compatible interaction respectively, in response to Puccinia triticina induced leaf-rust infection. Taken together, our study suggests a role for SNARE genes in vesicle mediated resistance to leaf rust in wheat.

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小麦SNARE基因的全基因组鉴定、克隆、鉴定及其对叶锈病的反应
SNAREs(可溶性n -乙基马来酰亚胺敏感因子适配蛋白受体)是一种小多肽,具有一个称为SNARE基序的特定结构域。与其他真核生物的基因组相比,单子叶和双子叶植物具有更多的SNAREs,这表明它们在高等植物物种中的重要作用。高等植物具有形成SNARE复合物的能力,这对于确定细胞内运输途径中囊泡融合的精确过程非常重要。据报道,在细胞分裂过程中,SNAREs参与细胞壁前体向新形成的细胞板的传递。SNARE基因在植物与病原体相互作用中的作用尚不清楚。我们使用隐马尔可夫模型在小麦基因组中发现了35个SNARE基因。在本研究中,我们结合硅技术和分子克隆技术,鉴定了三个SNARE基因(SNARE3、SNARE5和SNARE6)。这些SNARE基因的氨基酸序列包含两个特征保守结构域- SNARE基序和跨膜结构域,并与其他真核SNARE基因具有高度的同源性。系统发育分析和在modeler软件的帮助下建立的三维结构证实了SNARE基序在蛋白质中的存在。时空表达谱研究表明,在小麦锈菌诱导的叶锈病侵染过程中,SNARE转录本分别在抗性和敏感性植株中发挥积极作用。综上所述,我们的研究表明SNARE基因在小麦囊泡介导的叶锈病抗性中起作用。
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Agri Gene
Agri Gene Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
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期刊介绍: Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.
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