{"title":"A magnetic affinity approach to identify plant GABA-binding proteins","authors":"Jie Zou, Jingzhe Guo, Shisheng Li","doi":"10.3906/biy-1901-79","DOIUrl":null,"url":null,"abstract":"In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl). New probes produced by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl) can obtain useful information on GABA receptors in plants.","PeriodicalId":23358,"journal":{"name":"Turkish Journal of Biology","volume":"43 1","pages":"246 - 255"},"PeriodicalIF":1.1000,"publicationDate":"2019-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3906/biy-1901-79","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Turkish Journal of Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3906/biy-1901-79","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 1
Abstract
In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl). New probes produced by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl) can obtain useful information on GABA receptors in plants.
期刊介绍：
The Turkish Journal of Biology is published electronically 6 times a year by the Scientific and Technological
Research Council of Turkey (TÜBİTAK) and accepts English-language manuscripts concerning all kinds of biological
processes including biochemistry and biosynthesis, physiology and metabolism, molecular genetics, molecular biology,
genomics, proteomics, molecular farming, biotechnology/genetic transformation, nanobiotechnology, bioinformatics
and systems biology, cell and developmental biology, stem cell biology, and reproductive biology. Contribution is open
to researchers of all nationalities.
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