Ascorbic Acid Enhances the Metabolic Activity, Growth and Collagen Production of Human Dermal Fibroblasts Growing in Three-dimensional (3D) Culture

IF 1 Q3 MULTIDISCIPLINARY SCIENCES gazi university journal of science Pub Date : 2022-09-02 DOI:10.35378/gujs.1040277
Serkan Dikici
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引用次数: 2

Abstract

Tissue engineering (TE) enables developing functional synthetic substitutes to be replaced with damaged tissues and organs instead of the use of auto or allografts. A wide range of biomaterials is currently in use as TE scaffolds. Among these materials, naturally-sourced ones are favourable due to being highly biocompatible and supporting cell growth and function whereas synthetic ones are advantageous because of the high tunability on mechanical and physical properties as well as being easy to process. Alongside the advantages of synthetic polymers, they mostly show hydrophobic behaviour that limits biomaterial-cell interaction and consequently the functioning of the developed TE constructs. In this study, we assessed the impact of L-Ascorbic acid 2-phosphate (AA2P) on improving the culture of human dermal fibroblasts (HDFs) growing on a three-dimensional (3D) scaffold made of polycaprolactone by emulsion templating technique. Our results demonstrated that AA2P enhances the metabolic activity, growth, and collagen production of HDFs when supplemented to their growth medium at 50 µg/mL concentration. It showed a great potential to be used as a growth medium supplement to circumvent the disadvantages of culturing human cells on a synthetic biomaterial that is not favoured in default. AA2P's potential to improve cell growth and collagen deposition may prove an effective way to culture human cells on 3D PCL PolyHIPE scaffolds for various TE applications.
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抗坏血酸增强三维培养的人皮肤成纤维细胞的代谢活性、生长和胶原生成
组织工程(TE)能够开发出用受损组织和器官替代的功能性合成替代品,而不是使用自体或同种异体移植物。目前,广泛的生物材料被用作TE支架。在这些材料中,天然来源的材料是有利的,因为它们具有高度的生物相容性并支持细胞生长和功能,而合成材料则是有利的。除了合成聚合物的优点外,它们大多表现出疏水行为,限制了生物材料与细胞的相互作用,从而限制了所开发的TE构建体的功能。在本研究中,我们通过乳液模板技术评估了L-抗坏血酸2-磷酸(AA2P)对改善在聚己内酯制成的三维(3D)支架上生长的人真皮成纤维细胞(HDFs)培养的影响。我们的研究结果表明,当添加到50µg/mL浓度的HDFs生长培养基中时,AA2P可增强HDFs的代谢活性、生长和胶原蛋白生成。它显示出作为生长培养基补充剂的巨大潜力,以避免在默认情况下不受欢迎的合成生物材料上培养人类细胞的缺点。AA2P改善细胞生长和胶原沉积的潜力可能被证明是在3D PCL PolyHIPE支架上培养人类细胞的有效方法,用于各种TE应用。
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来源期刊
gazi university journal of science
gazi university journal of science MULTIDISCIPLINARY SCIENCES-
CiteScore
1.60
自引率
11.10%
发文量
87
期刊介绍: The scope of the “Gazi University Journal of Science” comprises such as original research on all aspects of basic science, engineering and technology. Original research results, scientific reviews and short communication notes in various fields of science and technology are considered for publication. The publication language of the journal is English. Manuscripts previously published in another journal are not accepted. Manuscripts with a suitable balance of practice and theory are preferred. A review article is expected to give in-depth information and satisfying evaluation of a specific scientific or technologic subject, supported with an extensive list of sources. Short communication notes prepared by researchers who would like to share the first outcomes of their on-going, original research work are welcome.
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