Laboratory diagnosis of nonpolio enteroviruses: A review of the current literature

IF 3.5 Q1 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Biosafety and Health Pub Date : 2023-04-01 DOI:10.1016/j.bsheal.2022.12.002
Tarek Itani , Vladislav Chalapa , Aleksandr Semenov , Aleksandr Sergeev
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引用次数: 0

Abstract

Infections by nonpolio enteroviruses (EVs) are highly prevalent, particularly among children and neonates, where they may cause substantial morbidity and mortality. Laboratory diagnosis of these viral infections is important in patient prognosis and guidance of clinical management. Although the laboratory diagnosis of nonpolio EVs is mainly based on molecular techniques, classical virus-isolation techniques are still used in reference laboratories. Other techniques, such as antigen detection and serology, are becoming obsolete and rarely used in diagnosis. An important part of diagnosis and surveillance of EV infections is viral typing by VP1 gene sequencing using conventional Sanger technique and more recently, full-genome next-generation sequencing. The latter allows the typing of all EVs, better investigation of EV outbreaks, detection of coinfection, and identification of severity markers in the EV genome.

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非脊髓灰质炎肠道病毒的实验室诊断:现有文献综述
非脊髓灰质炎肠道病毒(ev)感染非常普遍,特别是在儿童和新生儿中,它们可能导致大量发病率和死亡率。这些病毒感染的实验室诊断对患者预后和指导临床管理具有重要意义。虽然非脊髓灰质炎病毒的实验室诊断主要基于分子技术,但参考实验室仍使用经典的病毒分离技术。其他技术,如抗原检测和血清学,正在变得过时,很少用于诊断。利用传统的Sanger技术和新一代全基因组测序技术,通过VP1基因测序进行病毒分型,是EV感染诊断和监测的重要组成部分。后者允许对所有EV进行分型,更好地调查EV暴发,检测合并感染以及鉴定EV基因组中的严重标记。
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来源期刊
Biosafety and Health
Biosafety and Health Medicine-Infectious Diseases
CiteScore
7.60
自引率
0.00%
发文量
116
审稿时长
66 days
期刊最新文献
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