Optimization of modified QuEChERS method for extraction of selected pharmaceuticals from vegetable samples using HPLC

IF 1.3 4区化学 Q3 CHEMISTRY, MULTIDISCIPLINARY Bulletin of the Chemical Society of Ethiopia Pub Date : 2023-05-12 DOI:10.4314/bcse.v37i4.3

B. Tegegne, Bhagwan Singh Chandravanshi, F. Zewge, L. Chimuka

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Abstract

ABSTRACT. Optimized QuEChERS (quick, easy, cheap, effective, rugged and safe) method of sample preparation was employed for five selected pharmaceutical compounds on its advantage of simplicity, less extraction solvent and extraction time. Different parameters affecting the extraction efficiency of the target analyte was optimized. Since QuEChERS was developed for pesticides, uses of the method for pharmaceuticals need modification and evaluation of different parameters. Clean-up sorbents were evaluated for their matrix effect removal after extraction and a combination of d-SPE sorbent, MgSO4, PSA, C18 and diatomaceous earth found to be selective clean-up sorbent for the targeted compounds. The addition and amount of diatomaceous earth and EDTA during the clean-up and extraction steps, respectively, were also examined and the amount was optimized. Solvent type and composition, salt type and extraction time were also optimized and methanol, MgSO4 with NaCl salt and 5 min extraction time was obtained. The method was successfully applied to different vegetable samples collected from Addis Ababa, Ethiopia and Johannesburg, South Africa (carrot, cabbage and lettuce) and none of the target analytes were found in the sample investigated. The matrix effect study on vegetable samples collected was found very high that suppresses the signal during analysis. KEY WORDS: QuEChERS, EDTA, Pharmaceuticals, Vegetable, HPLC Bull. Chem. Soc. Ethiop. 2023, 37(4), 831-844. DOI: https://dx.doi.org/10.4314/bcse.v37i4.3

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改良QuEChERS高效液相色谱法提取蔬菜样品中选定药物的优化研究

摘要采用优化后的QuEChERS(快速、简便、廉价、有效、坚固、安全)方法对5种选定的药物化合物进行样品制备，该方法简便、提取溶剂少、提取时间短。对影响目标分析物提取效率的不同参数进行了优化。由于QuEChERS是为农药开发的，因此将该方法用于药物需要修改和评估不同的参数。对净化吸附剂萃取后的基质效应去除效果进行了评价，发现d-SPE吸附剂、MgSO4、PSA、C18和硅藻土的组合对目标化合物是选择性的净化吸附剂。考察了硅藻土和EDTA在净化和提取过程中的添加量和用量，并对其进行了优化。对溶剂类型、组成、盐种和提取时间进行了优化，得到甲醇、MgSO4、NaCl盐和5 min的提取时间。该方法成功地应用于从埃塞俄比亚亚的斯亚贝巴和南非约翰内斯堡采集的不同蔬菜样品(胡萝卜、卷心菜和生菜)，在所调查的样品中没有发现目标分析物。对采集的蔬菜样品进行基质效应研究，发现基质效应对分析过程中的信号有很强的抑制作用。关键词:QuEChERS, EDTA，药品，蔬菜，高效液相色谱。化学。Soc。阿比西尼亚人。2023年,37 (4),831 - 844 . DOI: https://dx.doi.org/10.4314/bcse.v37i4.3

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来源期刊

Bulletin of the Chemical Society of Ethiopia 化学-化学综合

CiteScore

2.20

自引率

8.30%

发文量

113

审稿时长

6-12 weeks

期刊介绍： The Bulletin of the Chemical Society of Ethiopia (BCSE) is a triannual publication of the Chemical Society of Ethiopia. The BCSE is an open access and peer reviewed journal. The BCSE invites contributions in any field of basic and applied chemistry.