Effect of Four Antioxidants Combination on Number of Sertoli and Leydig Cells, Sperm Quality and Caspase-3 Expression in Rat Exposed to Cigarette Smoke

Conita Yuniarifa, Joko Wahyu Wibowo, T. Nasihun
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Abstract

Introduction : The administration of antioxidant combination has been shown to be more effective than that of individual antioxidant in male infertility. Objective : to determine the effect of different combination of antioxidants (vitamin/vit C, vit E, glutathione and zinc) on the number of Sertoli and Leydig cells, sperm quality and texpression of caspase-3 in rats exposed to cigarette smoke. Methods : This was an experimental study using a post test only control group design. Thirty six rats were randomly divided into 6 groups to receive one of the following combinations of antioxidants: vit C and E, glutathione, zinc (T4-G); vit C and E, glutathione (T3-G); vit C and vit E (T2-G); Glutathione, zinc (T1-G); control positive (CP-G) cigarette smoke exposed only, normal (CN-G). The dose of vit C, vit E, glutathion and zinc was 9 mg/day, 1.8 IU/day, 1.8 mg/day, 0.2 mg/day respectively. All groups were exposed to cigarette smoke (3 cigarettes/day for 21 days), except for the CN-G group. On day 22, sperm samples were taken. Testicular tissue was taken for measurements of sperm quality, sertoli and leydig cell.numbers. Results : There was a significant difference in mean number of Leydig cells, sperm motility, sperm count and caspase-3 between groups (p<0.05). Post hoc LSD showed that the administration of combination of four antioxidant agent increasing the number of Sertoli cells and sperm morphology. The results also showed that group treated with 4 antioxidants combination had the highest number of Leydig cells, sperm motility, sperm counts and the lowest expression of caspase-3. Conclusion :The combination of vitC, vit E, glutathione and zinc affects the number of Sertoli and Leydig cells, sperm quality, expression of caspase-3 in rats exposed to cigarette smoke .
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四种抗氧化剂组合对暴露于香烟烟雾大鼠睾丸和间质细胞数量、精子质量和Caspase-3表达的影响
在男性不育症中,抗氧化剂联合使用已被证明比单独使用抗氧化剂更有效。目的:探讨不同组合抗氧化剂(维生素/维生素C、维生素E、谷胱甘肽和锌)对暴露于香烟烟雾大鼠睾丸细胞和Leydig细胞数量、精子质量和caspase-3表达的影响。方法:采用单纯后测对照组设计的实验研究。将36只大鼠随机分为6组,给予以下抗氧化剂组合之一:维生素C和E、谷胱甘肽、锌(T4-G);维生素C和E,谷胱甘肽(T3-G);维生素C和维生素E (T2-G);谷胱甘肽锌(T1-G);对照阳性(CP-G)仅暴露于香烟烟雾,正常(CN-G)。维生素C、维生素E、谷胱甘肽、锌的剂量分别为9 mg/d、1.8 IU/d、1.8 mg/d、0.2 mg/d。除CN-G组外,其余各组均暴露于香烟烟雾中(3支/天,连续21天)。第22天,采集精子样本。取睾丸组织,测定精子质量、支持层和睾丸间质细胞数量。结果:各组间质细胞数、精子活力、精子数、caspase-3均有显著性差异(p<0.05)。事后LSD显示,四种抗氧化剂联合施用可增加支持细胞数量和精子形态。结果还表明,4种抗氧化剂组合处理组间质细胞数量、精子活力、精子数量最高,caspase-3表达最低。结论:维生素c、维生素E、谷胱甘肽和谷胱甘肽与锌联用可影响吸烟大鼠睾丸、间质细胞数量、精子质量及caspase-3的表达。
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