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Effect of Four Antioxidants Combination on Number of Sertoli and Leydig Cells, Sperm Quality and Caspase-3 Expression in Rat Exposed to Cigarette Smoke 四种抗氧化剂组合对暴露于香烟烟雾大鼠睾丸和间质细胞数量、精子质量和Caspase-3表达的影响
Pub Date : 2021-01-04 DOI: 10.30659/SAINSMED.V11I2.2465
Conita Yuniarifa, Joko Wahyu Wibowo, T. Nasihun
Introduction : The administration of antioxidant combination has been shown to be more effective than that of individual antioxidant in male infertility. Objective : to determine the effect of different combination of antioxidants (vitamin/vit C, vit E, glutathione and zinc) on the number of Sertoli and Leydig cells, sperm quality and texpression of caspase-3 in rats exposed to cigarette smoke. Methods : This was an experimental study using a post test only control group design. Thirty six rats were randomly divided into 6 groups to receive one of the following combinations of antioxidants: vit C and E, glutathione, zinc (T4-G); vit C and E, glutathione (T3-G); vit C and vit E (T2-G); Glutathione, zinc (T1-G); control positive (CP-G) cigarette smoke exposed only, normal (CN-G). The dose of vit C, vit E, glutathion and zinc was 9 mg/day, 1.8 IU/day, 1.8 mg/day, 0.2 mg/day respectively. All groups were exposed to cigarette smoke (3 cigarettes/day for 21 days), except for the CN-G group. On day 22, sperm samples were taken. Testicular tissue was taken for measurements of sperm quality, sertoli and leydig cell.numbers. Results : There was a significant difference in mean number of Leydig cells, sperm motility, sperm count and caspase-3 between groups (p<0.05). Post hoc LSD showed that the administration of combination of four antioxidant agent increasing the number of Sertoli cells and sperm morphology. The results also showed that group treated with 4 antioxidants combination had the highest number of Leydig cells, sperm motility, sperm counts and the lowest expression of caspase-3. Conclusion :The combination of vitC, vit E, glutathione and zinc affects the number of Sertoli and Leydig cells, sperm quality, expression of caspase-3 in rats exposed to cigarette smoke .
在男性不育症中,抗氧化剂联合使用已被证明比单独使用抗氧化剂更有效。目的:探讨不同组合抗氧化剂(维生素/维生素C、维生素E、谷胱甘肽和锌)对暴露于香烟烟雾大鼠睾丸细胞和Leydig细胞数量、精子质量和caspase-3表达的影响。方法:采用单纯后测对照组设计的实验研究。将36只大鼠随机分为6组,给予以下抗氧化剂组合之一:维生素C和E、谷胱甘肽、锌(T4-G);维生素C和E,谷胱甘肽(T3-G);维生素C和维生素E (T2-G);谷胱甘肽锌(T1-G);对照阳性(CP-G)仅暴露于香烟烟雾,正常(CN-G)。维生素C、维生素E、谷胱甘肽、锌的剂量分别为9 mg/d、1.8 IU/d、1.8 mg/d、0.2 mg/d。除CN-G组外,其余各组均暴露于香烟烟雾中(3支/天,连续21天)。第22天,采集精子样本。取睾丸组织,测定精子质量、支持层和睾丸间质细胞数量。结果:各组间质细胞数、精子活力、精子数、caspase-3均有显著性差异(p<0.05)。事后LSD显示,四种抗氧化剂联合施用可增加支持细胞数量和精子形态。结果还表明,4种抗氧化剂组合处理组间质细胞数量、精子活力、精子数量最高,caspase-3表达最低。结论:维生素c、维生素E、谷胱甘肽和谷胱甘肽与锌联用可影响吸烟大鼠睾丸、间质细胞数量、精子质量及caspase-3的表达。
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引用次数: 0
The Effect of Antlion (Myrmeleon sp.) Extract Towards Histopathology Image of Pancreas in Diabetic Mice 蚁提取物对糖尿病小鼠胰腺组织病理学图像的影响
Pub Date : 2021-01-04 DOI: 10.30659/SAINSMED.V11I2.7656
Muhammad Naufal Widyatmaka, Akhmad Ismail
Introduction: Some studies indicated that antlions (Myrmeleon sp.) is traditionally used by many people in Indonesia as a diabetes treatmenter. However. reseach has not been done to find the effect of antlion extract in pancreas, an important organ in diabetes mellitus pathophysiology. Objective: To prove the effect of antlion extract towards histopathological feature of pancreas in diabetic mice.Methods: In the post test only control group design, 25 Swiss mice were divided into five groups. Normal group (Nor-G), Negative control group (Neg-G), and the other 3 groups were intraperitoneally injected with Streptozotocine 200 mg/kg to induce diabetic state. In Neg-G had no antlion treatment, whereas in At-14, At-28, and At-56 were treated with antlion extract at the dose of 14, 28, and 56 mg/kg BW respectively for 24 days. Pancreas then collected and prepared to be examined under microscope with 400x magnification. The score of pancreatic islet injury was determined by assessing structural damage and inflammatory cells infiltration. Kruskal-Wallis and Mann-Whitney were adopted as statistical analysis.Results: Mann-Whitney analysis indicated that the score of pancreatic islets injury in At-56 was significant lower compared to that of Neg-G, p<0.05. The score of pancreatic islets injury in At-56 also significant lower compared to that of At-14 and At-28, p<0.05. In contrary, when compared to that of Nor-G, the pancreatic islets injury in At-56 was not signicantly lower, p>0.05.Conclusion: Antlion extract treatment at the dose of 56 mg/kg BW was capable of reducing pancreatic islet injury in diabetic mice induced by streptozotocine.
引言:一些研究表明,蚂蚁(Myrmeleon sp.)在印度尼西亚传统上被许多人用作糖尿病治疗药物。然而蚂蚁提取物对糖尿病病理生理学中的重要器官胰腺的作用尚未得到研究。目的:证实蚂蚁提取物对糖尿病小鼠胰腺组织病理学特征的影响。方法:在仅试验后的对照组设计中,25只瑞士小鼠被分为5组。正常组(Nor-G)、阴性对照组(Neg-G)和其他3组腹膜内注射链脲佐菌素200mg/kg以诱导糖尿病状态。在Neg-G中没有蚂蚁处理,而在At-14、At-28和At-56中分别用蚂蚁提取物以14、28和56mg/kg BW的剂量处理24天。然后收集胰腺,准备在放大400倍的显微镜下进行检查。通过评估结构损伤和炎症细胞浸润来确定胰岛损伤的评分。采用Kruskal-Wallis和Mann-Whitney作为统计分析。结果:Mann-Whitney分析表明,At-56对胰岛损伤的评分显著低于Neg-G,p0.05。结论:蚂蚁提取物56mg/kg BW可减轻链脲佐菌素诱导的糖尿病小鼠胰岛损伤。
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引用次数: 0
CMCE Propolis Extract Improve Caspase 3 Expressions of The Hepatocytes and IL-6 Levels in Rats Exposed with CCl4 CMCE蜂胶提取物提高CCl4暴露大鼠肝细胞Caspase 3表达及IL-6水平
Pub Date : 2021-01-04 DOI: 10.30659/SAINSMED.V11I2.5190
Chalid Firdaus, Chodidjah Chodidjah, T. Nasihun
Introduction : Liver plays an important role in the metabolic processes possibly exposed to the toxic materials. One of those hepatotoxic substances is carbon tetrachloride. The propolis extract contains the balsamic active substances, CAPE compounds and flavonoids. Flavonoids can prevent from the apoptosis and reduce the inflammation. Objective : To validate propolis extract’s (CMCE method) ability in improving caspase 3 expressions of hepatocytes and IL-6 levels in Wistar male rats induced with carbon tetrachloride. Methods : An experimental study with a post-test only control group design applied to the study. The research subjects were 28 male Wistar rats, divided into four groups. The negative control group (CN-G) was only injected with CCl4, while the experimental groups were administered with the CMCE propolis at the dosages of 3.6 mg/200g (CM3-G), 7.2 mg/200g (CM7-G), and 14.4 mg/200g (CM14-G). The CMCE propolis extract was administered for 14 days and on day 14, the CCl4 was then given. The caspase 3 expression of hepatocytes was measured using HE liver cell preparations, while the IL-6 levels were measured using ELISA method. Results : The results of Mann-Whitney U statistical analysis showed that the hepatocytes’ caspase 3 expressions in CM14-G (2.77 ± 0.531), CM7-G (3.14 ± 0.378), and CM3-G (4.22 ± 0.690) were lower than those in CN-G (5.43 ± 0.535), p<0.05. Meanwhile, the Post Hoc LSD analysis Results showed that IL-6 levels in CM14-G (55.032 ± 9.336), CM7-G (78.362 ± 8.313), and CM3-G (114.975 ± 10.359) were lower those in CN-G (180.301 ± 5.428), p<0.05. Conclusion : the administration of propolis extract (CMCE method) improve the caspase 3 expressions of hepatocytes and Il-6 levels in Wistar male rats induced with carbon tetrachloride.
引言:肝脏在可能接触有毒物质的代谢过程中起着重要作用。其中一种肝毒性物质是四氯化碳。蜂胶提取物含有香脂活性物质、CAPE化合物和黄酮类化合物。黄酮类化合物可以防止细胞凋亡,减少炎症反应。目的:验证蜂胶提取物(CMCE法)对四氯化碳诱导的Wistar雄性大鼠肝细胞胱天蛋白酶3表达和IL-6水平的改善作用。方法:采用试验后对照组设计进行实验研究。研究对象为28只雄性Wistar大鼠,分为四组。阴性对照组(CN-G)仅注射CCl4,而实验组以3.6 mg/200g(CM3-G)、7.2 mg/200g和14.4 mg/200g的剂量给药CMCE蜂胶。给予CMCE蜂胶提取物14天,然后在第14天给予CCl4。使用HE肝细胞制剂测量肝细胞的胱天蛋白酶3表达,而使用ELISA方法测量IL-6水平。结果:Mann-Whitney U统计分析结果显示,CM14-G(2.77±0.531)、CM7-G(3.14±0.378)和CM3-G(4.22±0.690)中肝细胞胱天蛋白酶3的表达低于CN-G(5.43±0.535),p<0.05。同时,事后LSD分析结果显示,CM14-G(55.032±9.336)、CM7-G(78.362±8.313)和CM3-G(114.975±10.359)的IL-6水平低于CN-G(180.301±5.428),p<0.05。结论:蜂胶提取物(CMCE法)可改善四氯化碳诱导的Wistar雄性大鼠肝细胞胱天蛋白酶3的表达和Il-6的水平。
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引用次数: 0
Purslane (Portulaca oleracea) Ethanol Extract decreases hs-CRP levels and Total Score of Renal Tubular Degeneration in Rat Model of Gentamicine-Induced Renal Damage 马齿苋乙醇提取物降低庆大霉素致肾损伤大鼠hs-CRP水平及肾小管变性总评分
Pub Date : 2021-01-04 DOI: 10.30659/SAINSMED.V11I2.4092
Ragil Saptaningtyas, Shofa Chasani, T. Nasihun
Introduction: Injection of intraperitoneal gentamicin at a dose of 60 mg/kg BW intra-peritoneal in rats for 7 days has been shown to trigger renal tubular degeneration and increase in high sensitivity C-reactive protein (hs-CRP) level. The use of purslane (Portulaca oleracea) to reduce hs-CRP levels and total score of renal tubular degeneration has not been reported.Objective: to determine the effect of purslane ethanol extract on hs-CRP levels and renal tubular degeneration score in gentamicin induced-renal damage in rats.Methods: This was a study using posttest only control group design. Twentyfive male Wistar rats aged 8-12 weeks, weighed 150-200 grams were allocated into 5 groups: normal (Nor-G), without treatment; negative (Neg-G), gentamicin (60 mg/kg BW), intraperitoneally; P-200; P-300 ; P-400 (gentamicin plus purslane extract at a dose of 200, 300, and 400 respectively) for 7 days. The hs-CRP was evaluated using the ELISA method. Total score of renal tubular degeneration was evaluated using modified score of Sarjadi.Results: there was a significant difference in mean hsCRP level and total score of renal tubular degeneration between groups (p <0.05). Posthoc analysis showed that hsCRP level and total score group of P-200, P-300 and P-400 were significantly lower than those of Neg-G, p <0.05. Meanwhile, the levels of hsCRP and total score of renal tubular degeneration in P-200, P-300, P-400, and Nor-G groups were not significantly different (p> 0.05).Conclusion: the administration of purslane ethanol extract at doses of 200, 300, and 400 mg/kg BW for 7 days improve hs-CRP level and total score of tubular degeneration similar to normal. 
引言:大鼠腹膜内注射庆大霉素,剂量为60 mg/kg BW,持续7天,已被证明会引发肾小管变性和高敏C反应蛋白(hs-CRP)水平升高。马齿苋(Portulaca oleracea)用于降低hs-CRP水平和肾小管变性总分的研究尚未报道。目的:观察马齿苋乙醇提取物对庆大霉素肾损伤大鼠hs-CRP水平和肾小管变性评分的影响。方法:这是一项仅使用后测对照组设计的研究。年龄8-12周,体重150-200克的雄性Wistar大鼠25只,分为5组:正常(Nor-G),不治疗;阴性(阴性-G),庆大霉素(60 mg/kg BW),腹膜内;P-200;P-300;P-400(庆大霉素加马齿苋提取物,剂量分别为200、300和400)持续7天。hs-CRP采用ELISA法进行评价。结果:各组间hsCRP平均水平和肾小管变性总分差异有统计学意义(p0.05),400mg/kg BW持续7天可改善hs-CRP水平和肾小管变性总分,与正常人相似。
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引用次数: 1
Senam Sehat Anak Indonesia Improve Peak Expiratory Flow Rate in Children with Obesity 改善肥胖儿童的呼气流量峰值
Pub Date : 2021-01-04 DOI: 10.30659/SAINSMED.V11I2.7598
F. Ramadhania, Rahmi Isma Asmara Putri, Erna Setiawati, Yetty M Nency
Introduction: Excessive fat accumulation in the obese child causes complex problems in the respiratory system. Respiratory system function can be improved by exercise. One exercise model that is suitable for children is Senam Sehat Anak Indonesia (SSAI). The quality of the respiratory function system is measured using Peak Expiratory Flow Rate (PEFR).Objective: to validate the PEFR’s differences in obese children before and after performing SSAI.Methods: This research using one group pre-posttest experimental design with total sample of 20 obese children using purposive sampling method. The research instrument uses weight scale, height scale, and peak flow meter. SSAI was given to all subjects twice a week for six weeks. Paired T Test statistical analysis was used to examine the PEFR’s differences before and after performing SSAI, considered significant if p<0.05.Results: The PEFR’s mean of all subjects before performing SSAI was 258.25 ± 39.51 L∕minute, while PEFR’s mean after performing SSAI twice a week for 6 weeks was 282.50 ± 43.03 L∕minute, (p=0,000).Conclusion: The PEFR’s mean of obese children was increase significantly after performing SSAI twice a week for 6 weeks
肥胖儿童体内过多的脂肪堆积会引起呼吸系统的复杂问题。通过运动可以改善呼吸系统的功能。一种适合儿童的运动模式是Senam Sehat Anak Indonesia (SSAI)。呼吸功能系统的质量是用呼气流量峰值(PEFR)来衡量的。目的:验证肥胖儿童SSAI手术前后PEFR的差异。方法:本研究采用一组前测后实验设计,共抽样20例肥胖儿童,采用目的抽样法。研究仪器采用重量秤、高度秤、峰值流量计。所有受试者每周给予SSAI两次,持续六周。采用配对T检验统计分析SSAI前后PEFR的差异,p<0.05认为差异有统计学意义。结果:所有受试者在进行SSAI前的PEFR平均值为258.25±39.51 L / min,而每周进行两次SSAI后的PEFR平均值为282.50±43.03 L / min,连续6周,p= 0000。结论:每周进行2次SSAI,连续6周后,肥胖儿童PEFR均值明显升高
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引用次数: 0
The Administration of Topical Aloe vera Extract Reduce the Number of Sunburn Cells and Expression of Caspase-3 on Post UVB-light-exposure Epidermis 局部施用芦荟提取物可减少UVB光照后表皮上晒伤细胞的数量和半胱天冬酶-3的表达
Pub Date : 2021-01-04 DOI: 10.30659/SAINSMED.V11I2.9094
Kusumawati Soetrisno, P. Subchan, Atina Hussana
Introduction : Ultraviolet B (UVB) radiation triggers the formation of free radicals that cause apoptosis and sunburn cells (SBC) formation. Aloe vera contains anti-inflammatory and antioxidant compounds that can potentially inhibit this process. Objective : to assess the effect of topical Aloe vera extract administration on the decrease of SBC number and Caspase-3 expression on the epidermis after UVB light exposure. Methods : In a post-test only group design study, a single dose of 3 doses of Erythema (DEM) was performed on two groups of 6 week old BALB/c female rats. Group A (control) did not receive any topical treatment, and group B (treatment) were smeared with 75% Aloe vera extract before irradiation. Each group was divided into 4 sub-groups based on post-exposure time of 6 hours (A1 and B1), 12 hours (A2 and B2), 24 hours (A3 and B3), and 48 hours (A4 and B4). The expression of caspase-3 was assessed by immunohistochemical staining while the SBC number was measured using a microscope. The expression of caspase-3 was analyzed using the Kruskal-Wallis and Mann-Whitney statistical tests, while the number of SBC was analyzed using the one-way ANOVA statistical test and the post-hoc LSD test. Results : The results of the Kruskal-Wallis analysis showed a significant difference in Caspase-3 expression between groups, p <0.05. The results of Mann-Whitney analysis showed a significant difference in Caspase-3 expression between the A1-A3 and B1-B3 subgroups (p <0.05). One-way ANOVA analysis showed a significant difference in the number of SBC between groups (p <0.05). Post-hoc LSD analysis showed significant differences in SBC counts between groups A and B across all subgroups, p <0.05. Conclusion : Topical application of Aloe Vera extract decreased Caspase-3 expression and the number of SBC in UVB light-exposed skin.
简介:紫外线B(UVB)辐射会触发自由基的形成,从而导致细胞凋亡和晒伤细胞(SBC)的形成。芦荟含有抗炎和抗氧化化合物,可能会抑制这一过程。目的:评价芦荟提取物局部给药对UVB照射后表皮SBC数量和Caspase-3表达减少的影响。方法:在一项仅试验后的组设计研究中,对两组6周龄的BALB/c雌性大鼠进行单剂量3剂的红斑(DEM)治疗。A组(对照组)未接受任何局部治疗,B组(治疗组)在照射前涂抹75%芦荟提取物。根据暴露后6小时(A1和B1)、12小时(A2和B2)、24小时(A3和B3)和48小时(A4和B4),每组分为4个子组。通过免疫组织化学染色评估胱天蛋白酶-3的表达,同时使用显微镜测量SBC数量。使用Kruskal-Wallis和Mann-Whitney统计检验分析胱天蛋白酶-3的表达,而使用单因素方差分析统计检验和事后LSD检验分析SBC的数量。结果:Kruskal-Wallis分析结果显示,各组间Caspase-3的表达存在显著差异,p<0.05。Mann-Whitney分析结果显示,A1-A3和B1-B3亚组的Caspase-3表达存在显著差异(p<0.05)。单向ANOVA分析显示,两组之间的SBC数量有显著差异(p>0.05)。事后LSD分析显示,a组和B组之间所有亚组的SBC计数有显著差异,p<0.05。结论:芦荟提取物局部应用可降低UVB光照皮肤中Caspase-3的表达和SBC的数量。
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引用次数: 3
Comparison between Insulin-like Growth Factor Binding Protein-1 Level and Bishop Score as Predictor of Successful Labor Induction in Full Term Pregnancy with Preeclampsia 胰岛素样生长因子结合蛋白-1水平与Bishop评分作为先兆子痫足月妊娠引产成功预测指标的比较
Pub Date : 2021-01-04 DOI: 10.30659/SAINSMED.V11I2.10919
Nurul Setiyorini, Ratna Cahyanti
Introduction: preeclampsia/eclampsia contributed to 30% of maternal mortality in RSUP Dr. Kariadi. Pre-induction bishop score assessment is a standard for cervical maturity estimation. Other predictors currently being developed, including insulin-like growth factor binding protein-1 (IGFBP-1). However, there is no cut-off point for IGFBP-1 examination in full term pregnancy, especially with preeclampsia and its complications. Objective: to find IGFBP-1 levels and Bishop Score differences in preeclampsia women with induction labor.Methods: this prospective observational cohort study was conducted in preeclampsia women with gestational age ≥37 weeks planned labor induction at Obstetrics and Gynecology Ward RSUP Dr. Kariadi Semarang and networking hospital during study period (n=66). History taking, physical examination, proteinuria, cervical mucus IGFBP-1, and bishop score calculation was performed. Statistical analysis was performed with paired-t test, followed by ROC for cut-off value of successful induction prediction, and logistic regression to determine confounding variables effect on IGFBP-1 levels.Results: The average Bishop score of 66 subjects was 2.5±1.81 and 2.6±1.8 for women who deliver ≤24 labor and in labor for ≤12 hours, respectively. Although the bishop score was higher in the successful labor group, there was no significant difference between groups. Mean IGFBP-1 value was 8.29±5.033 mcg/L with median value of 10.8 mcg/L. Successful induction had significant higher IGFBP-1 levels with area-under-curve (AUC) 0.76 and 8.145 cutoff value (p=0.002, RR=5.1).Conclusion: IGFBP-1 level with cutoff point 8.145 can be used as predictor of successful labor induction in term pregnancy with preeclampsia.
引言:子痫前期/子痫占RSUP产妇死亡率的30%。诱导前bishop评分评估是宫颈成熟度评估的标准。目前正在开发的其他预测因子包括胰岛素样生长因子结合蛋白-1 (IGFBP-1)。然而,在足月妊娠,特别是子痫前期及其并发症中,IGFBP-1检查没有分界点。目的:探讨IGFBP-1水平及Bishop评分在引产子痫前期妇女中的差异。方法:这项前瞻性观察队列研究在研究期间在RSUP妇产科病房Kariadi Semarang医生和网络医院计划引产的子痫前期妇女中进行(n=66)。进行病史记录、体格检查、蛋白尿、宫颈粘液IGFBP-1及bishop评分计算。统计学分析采用配对t检验,诱导预测成功的截断值采用ROC, logistic回归确定混杂变量对IGFBP-1水平的影响。结果:66例产妇分娩≤24小时和产程≤12小时的平均Bishop评分分别为2.5±1.81分和2.6±1.8分。虽然成功劳动组的主教得分较高,但组间无显著差异。IGFBP-1的平均值为8.29±5.033 mcg/L,中位数为10.8 mcg/L。诱导成功后IGFBP-1水平显著升高,曲线下面积(AUC)为0.76,截止值为8.145 (p=0.002, RR=5.1)。结论:IGFBP-1水平(临界值8.145)可作为足月妊娠合并子痫前期引产成功的预测指标。
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引用次数: 0
The Effect of Honey in Second Degree Burn Healing on Wistar Rats (Overview of Angiogenesis and the Number of Fibroblasts) 蜂蜜对Wistar大鼠二度烧伤愈合的影响(血管生成及成纤维细胞数量综述)
Pub Date : 2020-09-13 DOI: 10.30659/SAINSMED.V11I1.7614
Ayyasi Izaz Almaz, R. D. Purnawati, H. Istiadi, N. Susilaningsih
Introduction: Burn therapy is generally done using a burn ointment. However, honey can also be used as an alternative treatment for burns. Honey contains active flavonoid as an antioxidant and hydrogen peroxide which can stimulate fibroblasts proliferation also angiogenesis on burn healing phase.Objective: to determine the effect of honey in second degree burn healing from angiogenesis and the number of fibroblasts on Wistar rats. Methods: This research used a posttest only controlled group design on 15 rats with random selection and divided into 3 groups: H-G was given honey, B-G was given Bioplacenton�, N-G without any treatment after burn was induced. Rats were given a second degree burn in a size of 2 cm x 2cm on the back area and were treated twice a day for 14 days. Burn healing was measured by calculating the observation results of microscopic VEGF expression, the number of neovascularization, and the number of fibroblasts. Hypothesis analysis used Annova post hoc LSD and Mann-Whitney.Results: The highest mean in VEGF expression and the number of neovascularization was obtained from group B-G followed by group H-G and group N-G. Significant differences in the number of neovascularization between group B-G with group N-G, p<0.05.Conclusion: Honey can provide a healing effect on the second degree of burns in Wistar rats in terms of the number of neovascularization as well as Bioplacenton�
简介:烧伤治疗通常使用烧伤膏。然而,蜂蜜也可以作为烧伤的替代治疗方法。蜂蜜含有活性类黄酮作为抗氧化剂和过氧化氢,过氧化氢可以刺激成纤维细胞增殖,也可以刺激烧伤愈合期的血管生成。目的:观察蜂蜜对Wistar大鼠二度烧伤血管生成及成纤维细胞数量的影响。方法:本研究采用随机选择的15只大鼠作为试验后对照组,分为3组:H-G组给予蜂蜜,B-G组给予Bioplacenton�, 诱导烧伤后不经任何处理的N-G。对大鼠背部进行2厘米x 2厘米大小的二度烧伤,每天治疗两次,持续14天。通过计算显微镜下VEGF表达、新生血管数量和成纤维细胞数量的观察结果来测量烧伤愈合。结果:B-G组VEGF表达和新生血管数量的平均值最高,其次是H-G组和N-G组。B-G组和N-G组的新生血管数量存在显著差异,p<0.05。结论:蜂蜜对Wistar大鼠二度烧伤新生血管数量和Bioplacenton均有一定的愈合作用�
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引用次数: 0
The Influence of Continuous Multi Stage Countercurrent Extraction Process (CMCE) Propolis Extract Administration on Superoxide Dismutase Activity and Hepatocytes Fibrosis Degree (An Experimental Study on Male Wistar Rats Induced with Carbon Tetrachloride (CCl4)) 连续多级逆流提取蜂胶提取物对超氧化物歧化酶活性和肝细胞纤维化程度的影响(四氯化碳诱导雄性Wistar大鼠的实验研究)
Pub Date : 2020-08-03 DOI: 10.30659/SAINSMED.V11I1.5000
Elsa Wani, T. Nasihun, Atina Hussaana
Introduction: Continuous Multi Stage Countercurrent Extraction Process (CMCE) propolis extract containing Caffeic Acid Phenethyl Ester (CAPE) may inhibit the formation of lipid peroxidation. However, it is still unclear whether or not CMCE propolis is able to increase SOD activity and reduce acute hepatocytes damages induced by CCl4. Objective: To evaluate the influence of CMCE propolis extract administration in increasing the SOD activity and reducing the hepatocytes fibrosis of male Wistar rats induced with CCl4. Method: an experimental research with a post-test only control group design. Twenty eight male Wistar rats were divided into four groups. Group C-N was only induced with CCl4, while group CM-3, CM-7, and CM-14 were induced with CCl4 and received CMCE propolis extract respectively with 3.6 mg/200 g BW, 7.2 mg/200 g BW, and 14.4 mg/200 g BW. CMCE propolis was administered for 14 days and then CCl4 was administered on the last day. SOD activity was measured using the colorimetric method, while the hepatocytes fibrosis degree was assessed using Image J Software method with NASH score modification. Results: Post Hoc LSD analysis indicated that SOD activity in group CM-3 (51.67 ± 4.20), CM-7 (70.21 ± 6.26), CM-14 (80.85 ± 4.59) was lower than that in group C-N (23.70 ± 5.12) with P <0.05. Meanwhile, the Mann-Whitney U test showed that the hepatocytes fibrosis degree in group CM-3 (0.85 ± 0.69), CM-7 (1.14 ± 0.37), and CM-14 (0.71 ± 0.48) was significantly lower than that in group C-N (2.28 ± 1.25) with p<0.05. Conclusion: The CMCE propolis extract administration was able to increase the SOD activity and reduce the hepatocytes fibrosis degree of male Wistar rats induced with CCl4
前言:连续多级逆流萃取法(CMCE)含有咖啡酸苯酯(CAPE)的蜂胶提取物可抑制脂质过氧化的形成。然而,目前尚不清楚CMCE蜂胶是否能够提高SOD活性并减少CCl4诱导的急性肝细胞损伤。目的:评价羧甲基纤维素蜂胶提取物对CCl4诱导的雄性Wistar大鼠SOD活性及肝细胞纤维化的影响。方法:采用仅测试后的对照组设计进行实验研究。将28只雄性Wistar大鼠分为四组。C-N组仅用CCl4诱导,而CM-3、CM-7和CM-14组用CCl4诱导,并分别用3.6 mg/200 g BW、7.2 mg/200 g BW和14.4 mg/200 g BW接受CMCE蜂胶提取物。CMCE蜂胶给药14天,然后在最后一天给药CCl4。SOD活性采用比色法测定,肝细胞纤维化程度采用ImageJ软件法,NASH评分修正。结果:事后LSD分析表明,CM-3组(51.67±4.20)、CM-7组(70.21±6.26)、CM-14组(80.85±4.59)的SOD活性低于C-N组(23.70±5.12),P<0.05。同时,Mann-Whitney U检验显示,CM-3组(0.85±0.69)、CM-7组(1.14±0.37)和CM-14组(0.71±0.48)的肝细胞纤维化程度显著低于C-N组(2.28±1.25),p<0.05。结论:CMCE蜂胶提取物能提高CCl4诱导的雄性Wistar大鼠SOD活性,降低肝细胞纤维化程度
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引用次数: 0
The Immunomodulatory Effect of Cinnamon (Cinnamomum Burmanii) Bark Extract On the C-Reactive Protein (CRP) Level, Leukocyte Count and Leukocyte Type Count of Wistar Rats Exposed to Staphylococcus Aureus 肉桂(Cinnamomum Burmanii)树皮提取物对金黄色葡萄球菌暴露Wistar大鼠c反应蛋白(CRP)水平、白细胞计数和白细胞类型计数的免疫调节作用
Pub Date : 2020-07-05 DOI: 10.30659/SAINSMED.V11I1.4907
Astika Widy Utomo, D. Retnoningrum, A. Gumay
Introduction: Bacterial infection induces inflammation in human body. This process produceshumoral and cellular immune responses. Cinnamomum burmanii grows very vast in Indonesia and contains cinnamaldehyde known to have an anti-inflammatory effect. Objective : To prove the effect of C. burmanii bark extract on CRP level, leukocyte count and differential blood count. Methods:  Aposttest-only controlled group design with 25 Wistar Rats divided into 5 groups was employed. The CN-G group was giventhe standard feed, the CP-G group was given the standard feed and levamisole 2.5 mg/KgBW, while the CBE-100, CBE-200, and CBE-400 groups were respectively given the standard feed and cinnamon bark extract 100 mg/kgBW, 200 mg/KgBW and 400 mg/KgBW. The treatmentswereconducted for 7 consecutive days.On day 8, all rats were injected with the suspense of S. aureus intraperitoneally. The blood wasthen drawn on day 9, followed with CRP level measurement using the ELISA method. The total leukocyte count and differential blood count weremanually measured. Results:  There is no significant difference in the value of CRP level (One Way ANOVA; p = 0.749) with the total counts of leukocytes(p=0.685), monocytes (p=0.769), and eosinophil(p=0.123) between groups. The neutrophils and lymphocytes of CBE-100 group aresignificantly differentfrom the other groups. Conclusion:  C. burmanii extract has a potential benefit as immunomodulator.
简介:细菌感染可引起人体炎症。这一过程产生了肿瘤和细胞免疫反应。缅甸肉桂在印度尼西亚生长非常广泛,含有已知具有抗炎作用的肉桂醛。目的:验证缅甸苦皮提取物对血清C反应蛋白(CRP)、白细胞计数和差异血细胞计数的影响。方法:采用单纯离体试验对照组设计,将25只Wistar大鼠分为5组。CN-G组给予标准饲料,CP-G组给标准饲料和左旋咪唑2.5mg/KgBW,而CBE-100、CBE-200和CBE-400组分别给标准饲料、肉桂皮提取物100mg/KgBW、200mg/KgBW和400mg/KgBW。治疗连续7天。第8天,所有大鼠腹膜内注射金黄色葡萄球菌悬液。然后在第9天抽血,然后使用ELISA方法测量CRP水平。人工测量白细胞总数和差异血细胞计数。结果:各组间CRP水平(单向方差分析;p=0.749)与白细胞(p=0.685)、单核细胞(p=0.769)和嗜酸性粒细胞(p=0.123)的总计数无显著差异。CBE-100组中性粒细胞和淋巴细胞与其他组比较有显著性差异。结论:缅甸虫草提取物具有潜在的免疫调节剂作用。
{"title":"The Immunomodulatory Effect of Cinnamon (Cinnamomum Burmanii) Bark Extract On the C-Reactive Protein (CRP) Level, Leukocyte Count and Leukocyte Type Count of Wistar Rats Exposed to Staphylococcus Aureus","authors":"Astika Widy Utomo, D. Retnoningrum, A. Gumay","doi":"10.30659/SAINSMED.V11I1.4907","DOIUrl":"https://doi.org/10.30659/SAINSMED.V11I1.4907","url":null,"abstract":"Introduction: Bacterial infection induces inflammation in human body. This process produceshumoral and cellular immune responses. Cinnamomum burmanii grows very vast in Indonesia and contains cinnamaldehyde known to have an anti-inflammatory effect. Objective : To prove the effect of C. burmanii bark extract on CRP level, leukocyte count and differential blood count. Methods:  Aposttest-only controlled group design with 25 Wistar Rats divided into 5 groups was employed. The CN-G group was giventhe standard feed, the CP-G group was given the standard feed and levamisole 2.5 mg/KgBW, while the CBE-100, CBE-200, and CBE-400 groups were respectively given the standard feed and cinnamon bark extract 100 mg/kgBW, 200 mg/KgBW and 400 mg/KgBW. The treatmentswereconducted for 7 consecutive days.On day 8, all rats were injected with the suspense of S. aureus intraperitoneally. The blood wasthen drawn on day 9, followed with CRP level measurement using the ELISA method. The total leukocyte count and differential blood count weremanually measured. Results:  There is no significant difference in the value of CRP level (One Way ANOVA; p = 0.749) with the total counts of leukocytes(p=0.685), monocytes (p=0.769), and eosinophil(p=0.123) between groups. The neutrophils and lymphocytes of CBE-100 group aresignificantly differentfrom the other groups. Conclusion:  C. burmanii extract has a potential benefit as immunomodulator.","PeriodicalId":31696,"journal":{"name":"Sains Medika","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42405794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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Sains Medika
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