{"title":"Double-Immunodiffusion Assay for Detecting Specific Antibodies (Ouchterlony)","authors":"Peter Hornbeck","doi":"10.1002/cpim.18","DOIUrl":null,"url":null,"abstract":"<p>The method first described by Ouchterlony in 1948 is a classic and simple technique that permits evaluation and comparison of antibodies in animal or human sera directed against protein or complex carbohydrate antigens. It is a low-tech procedure that may provide information on the relative quantity of antibody activity and the nature of the antigenic epitopes in different preparations. © 2017 by John Wiley & Sons, Inc.</p>","PeriodicalId":10733,"journal":{"name":"Current Protocols in Immunology","volume":"116 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2017-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpim.18","citationCount":"14","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Immunology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpim.18","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Immunology and Microbiology","Score":null,"Total":0}
引用次数: 14
Abstract
The method first described by Ouchterlony in 1948 is a classic and simple technique that permits evaluation and comparison of antibodies in animal or human sera directed against protein or complex carbohydrate antigens. It is a low-tech procedure that may provide information on the relative quantity of antibody activity and the nature of the antigenic epitopes in different preparations. © 2017 by John Wiley & Sons, Inc.
双免疫扩散法检测特异性抗体
Ouchterlony于1948年首次描述的方法是一种经典而简单的技术,可以评估和比较动物或人类血清中针对蛋白质或复杂碳水化合物抗原的抗体。这是一种低技术含量的方法,可以提供不同制剂中抗体活性的相对数量和抗原表位性质的信息。©2017 by John Wiley &儿子,Inc。
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