Assessment of Genetic Diversity and Protein Content of Scandinavian Peas (Pisum sativum)

IF 3.3 2区 农林科学 Q1 AGRONOMY Agronomy-Basel Pub Date : 2023-08-31 DOI:10.3390/agronomy13092307
Louise Winther, Søren Kjærsgaard Rasmussen, Gert Poulsen, C. B. A. Lange
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Abstract

We produced homogeneous lines of 227 pea accessions from the Nordic Genetic Resource Center via single seed descent. The genetic diversity among these, mostly Scandinavian accessions, was investigated using three microsatellite markers, A9, AC58 and AA5. The microsatellites were highly informative and separated 153 of 194 accessions on a Neighbor Joining topology. The high polymorphism information content (PIC) values between 0.87 and 0.91 indicated that the gene bank material contains a large number of pea accessions with different breeding histories. The peas were grown in the field for two years and seed protein content showed variation between 9.3% and 34.1% over the years and accessions, respectively. The mean thousand seed weight was 152.05 g. More than 10 accessions had a protein content above 28%, showing that the collection has potential as breeding nursery for high-protein pea cultivars.
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北欧豌豆(Pisum sativum)遗传多样性和蛋白质含量评价
我们通过单种子杂交从北欧遗传资源中心获得了227份豌豆材料的同质系。使用三个微卫星标记A9、AC58和AA5对这些主要是斯堪的纳维亚材料的遗传多样性进行了研究。这些微卫星信息量很大,在相邻连接拓扑结构上分离了194份材料中的153份。多态性信息含量(PIC)在0.87和0.91之间的高值表明该基因库材料包含大量具有不同育种历史的豌豆材料。豌豆在田间生长两年,种子蛋白质含量随年份和材料的变化分别在9.3%和34.1%之间。平均千粒重为152.05g。超过10份材料的蛋白质含量超过28%,表明该集合具有作为高蛋白豌豆品种育种苗圃的潜力。
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来源期刊
Agronomy-Basel
Agronomy-Basel Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
6.20
自引率
13.50%
发文量
2665
审稿时长
20.32 days
期刊介绍: Agronomy (ISSN 2073-4395) is an international and cross-disciplinary scholarly journal on agronomy and agroecology. It publishes reviews, regular research papers, communications and short notes, and there is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.
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