Effects of combined Garcinia kola and Kigelia africana on Insulin and Paraoxonase 1 (PON1) levels in type 2 diabetic rats.

Q3 Pharmacology, Toxicology and Pharmaceutics Current drug discovery technologies Pub Date : 2022-04-08 DOI:10.2174/1570163819666220408100011
Omoaghe Adams O, Oyesola O, Ezike Tony, Omizu Blessing, B. Kukoyi
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Abstract

BACKGROUND Several reports of individual extracts of Garcina kola and Kigelia africana beneficial effects against several factors related with development of diabetes mellitus abound. However, there is still lack of information about the combined effects of these extracts on Insulin and Paraoxonase 1 (PON-1) in Streptozotocin-Nicotinamide induced type-2 diabetic Wistar rats. METHODS Forty-two young male rats (180-200g) were randomly divided into six groups (n=7/group). Diabetes was intraperitoneally induced with 110 mg/kg of nicotinamide constituted in distilled water andfifteen minutes after with 65mg/kg of streptozocin freshly prepared in 0.1M citrate buffer (pH of 4.5) and treated for six weeks as follows: the control rats received either 0.9% normal saline (NS) or 250mg/kg extract by gavage. The remaining animals were diabetic induced and subsequently treated with either NS, graded doses of the extract (250mg/kg and 500mg/kg) or 5mg/kg Glibenclamide + 100mg/kg Metformin. Gas chromatography mass spectrometry (GCMS) of the combined extracts was also analyzed to identify the bioactive compounds present. Insulin, PON-1 levels, lipid profiles and atherogenic index were assessed. RESULTS Our findings show that Insulin and PON-1 levels in the plasma of diabetic rats treated with the combined extracts were significantly increased when compared to the control rats. Moreover, the GCMS of the extract show the presence of both monosaturated (oleic acid) and polyunsaturated (linoleic acid) fatty acids. CONCLUSION The current findings suggest that the extract may help improve glucose homeostasis, prevent atherosclerosis through established mechanism of the identified bioactive compounds.
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藤黄和非洲猕猴桃联合用药对2型糖尿病大鼠胰岛素和对氧磷酶1(PON1)水平的影响。
背景:关于葛和非洲奇异果提取物对与糖尿病发展相关的几个因素的有益作用的报道比比皆是。然而,关于这些提取物对链脲佐菌素-烟酰胺诱导的2型糖尿病Wistar大鼠的胰岛素和对氧磷酶1(PON-1)的联合作用的信息仍然缺乏。方法2只雄性大鼠(180~200g),随机分为6组(n=7/组)。用110 mg/kg蒸馏水中的烟酰胺腹膜内诱导糖尿病,15分钟后用新制备的65mg/kg链脲佐菌素在0.1M柠檬酸盐缓冲液(pH 4.5)中诱导糖尿病,并按如下方式处理6周:对照大鼠通过灌胃接受0.9%生理盐水(NS)或250mg/kg提取物。其余动物被糖尿病诱导,随后用NS、分级剂量的提取物(250mg/kg和500mg/kg)或5mg/kg格列本脲+100mg/kg二甲双胍治疗。还分析了组合提取物的气相色谱-质谱(GCMS),以鉴定存在的生物活性化合物。评估胰岛素、PON-1水平、脂质分布和动脉粥样硬化指数。结果我们的研究结果表明,与对照大鼠相比,用联合提取物治疗的糖尿病大鼠血浆中的胰岛素和PON-1水平显著升高。此外,提取物的GCMS显示存在单饱和(油酸)和多不饱和(亚油酸)脂肪酸。结论目前的研究结果表明,该提取物可能通过所鉴定的生物活性化合物的既定机制,有助于改善葡萄糖稳态,预防动脉粥样硬化。
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来源期刊
Current drug discovery technologies
Current drug discovery technologies Pharmacology, Toxicology and Pharmaceutics-Drug Discovery
CiteScore
3.70
自引率
0.00%
发文量
48
期刊介绍: Due to the plethora of new approaches being used in modern drug discovery by the pharmaceutical industry, Current Drug Discovery Technologies has been established to provide comprehensive overviews of all the major modern techniques and technologies used in drug design and discovery. The journal is the forum for publishing both original research papers and reviews describing novel approaches and cutting edge technologies used in all stages of drug discovery. The journal addresses the multidimensional challenges of drug discovery science including integration issues of the drug discovery process.
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