Expressions of CD97 isoforms in colon cancer tissues and their clinical significances

Q4 Medicine 肿瘤研究与临床 Pub Date : 2019-10-28 DOI:10.3760/CMA.J.ISSN.1006-9801.2019.10.004

P. Peng, Chaohua Hu, Han Yuntao, Xu Yuanbing, Haoyuan Shen, Youlin Yu, Hongzhong Zhou

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引用次数: 1

Abstract

Objective To study the mRNA expressions of various CD97 isoforms in colorectal carcinoma tissues and their clinical significances. Methods A total of 50 colon cancer patients in the First Affiliated Hospital of Wenzhou Medical University from December 2013 to May 2014 and human colon cancer cell lines SW480 and SW620 were enrolled. The real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to detect the mRNA expressions of CD97 human epidermal growth factor (EGF) (1, 2, 5), CD97EGF (1, 2, 3, 5) and CD97EGF (1, 2, 3, 4, 5) in colon cancer tissues, adjacent tissues, normal colon tissues, SW480 cells and SW620 cells. The relationship between the mRNA expression of CD97EGF (1, 2, 5) and the clinicopathological factors was analyzed. Results Compared with those low expressions in adjacent tissues and normal tissues, the mRNA expressions of CD97 isoforms CD97EGF (1, 2, 5), CD97EGF (1, 2, 3, 5) and CD97EGF (1, 2, 3, 4, 5) in cancer tissues were highest, and the differences were statistically significant (0.71±0.20 vs. 0.40±0.09 vs. 0.35±0.07, F = 107.642, P < 0.01; 0.45±0.11 vs. 0.26±0.05 vs. 0.27±0.06, F = 94.231, P < 0.01; 0.41±0.10 vs. 0.21±0.05 vs. 0.19±0.03, F = 165.672, P < 0.01). In addition, the mRNA expression of CD97EGF (1, 2, 5) in colon cancer patients was associated with tumor infiltration depth (T1-T2 and T3-T4), clinical stages (Ⅰ-Ⅱ and Ⅲ-Ⅳ), and the differences were statistically significant (t = -2.582, P = 0.013; t = -5.062, P < 0.01). The mRNA expression of CD97EGF (1, 2, 5) in SW620 cells was higher than that in SW480 cells. Conclusions CD97 isoforms are highly expressed in colon cancer tissues, and CD97EGF (1, 2, 5) may play an important role in the development and invasion of colon cancer. The CD97 isoforms may be new markers in the treatment of colon cancer. Key words: Colonic neoplasms; Isoform, CD97; Reverse transcriptase polymerase chain reaction; Pathology, clinical

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CD97亚型在结肠癌组织中的表达及临床意义

目的研究CD97不同亚型mRNA在结直肠癌组织中的表达及其临床意义。方法选取2013年12月至2014年5月温州医科大学附属第一医院结肠癌患者50例，采用人结肠癌细胞系SW480和SW620。采用实时荧光定量聚合酶链反应(qRT-PCR)检测CD97人表皮生长因子(EGF)(1,2,5)、CD97EGF(1,2,3,5)和CD97EGF(1,2,3,4,5)在结肠癌组织、癌旁组织、正常结肠组织、SW480细胞和SW620细胞中的mRNA表达。分析CD97EGF (1,2,5) mRNA表达与临床病理因素的关系。结果CD97亚型CD97EGF(1、2、5)、CD97EGF(1、2、3、5)和CD97EGF(1、2、3、5)mRNA在癌组织中表达量最高，差异有统计学意义(0.71±0.20 vs. 0.40±0.09 vs. 0.35±0.07,F = 107.642, P < 0.01;0.45±0.11 vs. 0.26±0.05 vs. 0.27±0.06,F = 94.231, P < 0.01;0.41±0.10和0.21±0.05和0.19±0.03,F = 165.672, P < 0.01)。此外，CD97EGF (1,2,5) mRNA在结肠癌患者中的表达与肿瘤浸润深度(T1-T2和T3-T4)、临床分期(Ⅰ-Ⅱ和Ⅲ-Ⅳ)相关，差异均有统计学意义(t = -2.582, P = 0.013;t = -5.062, P < 0.01)。CD97EGF (1,2,5) mRNA在SW620细胞中的表达高于SW480细胞。结论CD97亚型在结肠癌组织中高表达，CD97EGF(1,2,5)可能在结肠癌的发生和侵袭过程中发挥重要作用。CD97亚型可能是结肠癌治疗的新标志物。关键词:结肠肿瘤;CD97同种型;逆转录酶聚合酶链反应;病理、临床

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来源期刊

肿瘤研究与临床 Medicine-Oncology

CiteScore

0.10

自引率

0.00%

发文量

7737

期刊介绍： "Cancer Research and Clinic" is a series of magazines of the Chinese Medical Association under the supervision of the National Health Commission and sponsored by the Chinese Medical Association. It mainly reflects scientific research results and academic trends in the field of malignant tumors. The main columns include monographs, guidelines and consensus, standards and norms, treatises, short treatises, survey reports, reviews, clinical pathology (case) discussions, case reports, etc. The readers are middle- and senior-level medical staff engaged in basic research and clinical work on malignant tumors.