Comparative evaluation of antioxidant activity of Cannabis sativa L. using FRAP and CUPRAP assays

IF 0.5 4区 化学 Q4 CHEMISTRY, MULTIDISCIPLINARY Chemija Pub Date : 2020-07-19 DOI:10.6001/chemija.v31i3.4289
A. Kubiliene, M. Marksa, J. Baranauskaite, O. Ragažinskienė, L. Ivanauskas
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引用次数: 4

Abstract

3 Kaunas Botanical Garden of Vytautas Magnus University, 4 Ž. A. Žiliberas Street, 46324 Kaunas, Lithuania Cannabis is one of the oldest plants on earth, which is known and used for medical purposes. There are many articles on hempseed oil research in the scientific databases, while the antioxidant activity of Cannabis sativa L. herb extracts has not been extensively studied yet, to our knowledge. In the present study, antioxidant properties of different Cannabis sativa L. varieties from different regions of Lithuania were examined. Spectrophotometric FRAP (ferric reducing antioxidant power) and CUPRAC (cupric reducing antioxidant capacity) methods were used for determination. It was found that Cannabis sativa L. herb extracts possess antioxidant activity. The strongest antioxidant activity was evaluated in the Futura variety and the lowest in Manoica. The obtained results showed that a statistically significant (p ˂ 0.05) higher reductive power was determined by analysing the raw material by the spectrophotometric FRAP method. According to the hemp growth location in Lithuania, the highest TEAC values were estimated in the samples from the North region.
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用FRAP和CUPRAP法比较评价大麻的抗氧化活性
3维陶塔斯马格纳斯大学考纳斯植物园,4 Ž。A. Žiliberas Street, 46324 Kaunas,立陶宛大麻是地球上最古老的植物之一,已知并用于医疗目的。科学数据库中关于大麻籽油研究的文章很多,但据我们所知,大麻提取物的抗氧化活性尚未得到广泛的研究。在本研究中,研究了立陶宛不同地区不同品种的大麻抗氧化性能。采用分光光度法FRAP(铁还原抗氧化能力)和CUPRAC(铜还原抗氧化能力)测定。研究发现,大麻提取物具有抗氧化活性。抗氧化活性最强的品种为Futura,最低的品种为Manoica。结果表明,采用FRAP分光光度法对原料进行分析,获得了具有统计学意义(p小于0.05)的高还原功率。根据立陶宛大麻的生长位置,估计来自北部地区的样品中TEAC值最高。
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来源期刊
Chemija
Chemija 化学-化学综合
CiteScore
1.30
自引率
16.70%
发文量
14
审稿时长
>12 weeks
期刊介绍: Chemija publishes original research articles and reviews from all branches of modern chemistry, including physical, inorganic, analytical, organic, polymer chemistry, electrochemistry, and multidisciplinary approaches.
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