Effects of Vitamin D in Alveolar Bone Remodeling on Osteoblast Numbers and Bone Alkaline Phosphatase Expression in Pregnant Rats During Orthodontic Tooth Movement
Puteri Nazirah Megat Badarul Hisham, I. Narmada, A. Alida, Dwi Rahmawati, A. Nugraha, N. Putranti
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引用次数: 10
Abstract
Introduction: The vitamin D effect on orthodontic tooth movement in pregnant women remains unknown. The aim of this study was to investigate the post administration of vitamin D effect on osteoblast numbers and bone alkaline phosphatase (BALP) expression in the tension side in pregnant rats during orthodontic tooth movement. Materials and Methods: This study was an in vivo animal experiment; 28 healthy female Wistar rats (Rattus norvegicus) (16–20 weeks’ old) were divided into four groups, with or without intramuscular administration of vitamin D, which were to be observed after 7 and 14 days. Pregnancy was induced with pregnant mare serum gonadotropin and human chorionic gonadotropin. Nickel–titanium coil springs with 30 g/mm2 of force were connected between the right maxillary incisors and the right maxillary first molar. After 7 and 14 days, the animals were sacrificed. Statistical Analysis Used: Analysis of variance with post hoc test (P < 0.05) was performed based on the results of a Levene’s test and a Kolmogorov–Smirnov test (P > 0.05). Results: The highest number of osteoblasts occurred in the C-7 group with mean ± standard deviation of 20.54 ± 8.4. Statistically significant differences were seen in decreased osteoblast number between groups (P = 0.001, P < 0.05). The highest BALP expression was in the E-7 group (3.40 ± 1.625). Nevertheless, no statistically significant difference was observed between the groups (P = 0.240, P > 0.05) in the expression of BALP. Conclusion: The post administration of vitamin D during orthodontic tooth movement in pregnant rats produced no significant enhancement on BALP expression and osteoblast number.
期刊介绍:
Journal of Orofacial Sciences is dedicated to noblest profession of Dentistry, and to the young & blossoming intellects of dentistry, with whom the future of dentistry will be cherished better. The prime aim of this journal is to advance the science and art of dentistry. This journal is an educational tool to encourage and share the acquired knowledge with our peers. It also to improves the standards and quality of therauptic methods. This journal assures you to gain knowledge in recent advances and research activities. The journal publishes original scientific papers with special emphasis on research, unusual case reports, editorial, review articles, book reviews & other relevant information in context of high professional standards.