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Comparative Digital Occlusal Analysis of Different Sagittal Skeletal Malocclusions in Dravidian Subjects − A Prospective Study 德拉威人不同矢状面骨错咬合的数字对比分析——一项前瞻性研究
Q3 Dentistry Pub Date : 2023-01-01 DOI: 10.4103/jofs.jofs_45_23
Swati Singh, R. Jain, Suresh Venugopalan, Aravind Prasanna
Introduction: T-scan III is used in clinical practice for a digital assessment of occlusal force distribution and other occlusal parameters. The present study was proposed to do an assessment of occlusal contacts and forces using T-scan III in subjects with class II division II malocclusion and compare it to subjects with class I malocclusion requiring fixed orthodontic treatment. Materials and Methods: This prospective study included 70 subjects − 33 in group A (Angle’s class II division II malocclusion) and 37 in group B (Angle’s class I malocclusion). Occlusal analysis was done with T-scan III, and parameters like occlusal force distribution (OFD), occlusion time (OT), and disocclusion time (DT) were recorded. Statistical analysis was done with SPSS software. Independent t-test for intergroup comparisons and paired t-tests for intra group comparisons were done. Results: A Significant intergroup difference in OFD between left/right, posterior/anterior regions (P < 0.05) was noted between groups. OT and DT (left lateral excursive) were also significantly higher (P < 0.05) in group A. On intergroup comparison, the L/R bite force ratio was higher in group B males (P < 0.05) and the P/A bite force ratio was higher in group B females (P < 0.05). Conclusion: In subjects with class II division II malocclusion, anterior teeth were subjected to greater occlusal loading when compared to class I subjects and had higher occlusion and disocclusion times.
引言:T扫描III在临床实践中用于咬合力分布和其他咬合参数的数字评估。本研究旨在使用T扫描III对II类II类错牙合患者的咬合接触和力进行评估,并将其与需要固定正畸治疗的I类错牙咬合患者进行比较。材料和方法:这项前瞻性研究包括70名受试者,其中A组33人(安氏II类II类错牙合),B组37人(安氏I类错牙)。使用T扫描III进行咬合分析,并记录咬合力分布(OFD)、咬合时间(OT)和咬合解除时间(DT)等参数。采用SPSS软件进行统计分析。组间比较采用独立t检验,组内比较采用配对t检验。结果:各组间左/右、后/前区的OFD差异有统计学意义(P<0.05)。OT和DT(左偏)在A组中也显著升高(P<0.05)。组间比较,B组男性的L/R咬合力比更高(P<0.05),B组女性的P/A咬合力比也更高(P<0.01),与I类受试者相比,前牙受到更大的咬合负荷,并且具有更高的咬合和不咬合时间。
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引用次数: 0
The dimensional insight: The role of cone beam computed tomography in Endodontics 维度洞察:锥形束计算机断层扫描在牙髓学中的作用
Q3 Dentistry Pub Date : 2023-01-01 DOI: 10.4103/jofs.jofs_200_23
K. Kacharaju
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引用次数: 0
Antimicrobial Activity of Simarouba glauca Leaf Extracts Against Oral Pathogens − An In Vitro Study 青叶提取物对口腔病原菌的体外抗菌活性研究
Q3 Dentistry Pub Date : 2023-01-01 DOI: 10.4103/jofs.jofs_147_22
S. Rudraswamy, Jai Shankar Hombarvali, M. Kenganora, N. Doggalli, B. Godhi, Sowmya Srinivas
Introduction: Oral diseases caused by biofilm continue to be a public health concern worldwide. The interesting task is to “battle” against oral biofilms, chiefly due to their propensity to persist even after mechanical removal. Mechanical oral hygiene measures, along with professional maintenance and usage of fluorides, are conservative practices to prevent oral biofilm. Adjunct to mechanical plaque control method, antimicrobial mouth wash is suggested. Although chlorhexidine is a gold standard antiplaque agent, its potential drawbacks on long-term use necessitates the development of a novel, alternate strategy that can inhibit oral biofilm. Materials and Methods: Simarouba glauca (SG) leaf extracts were prepared by maceration and Soxhlet methods. Zone of inhibition (ZOI) and Minimum inhibitory concentration (MIC) were conducted against Streptococcus mutans, Lactobacilli acidophilus, Staphylococcus aureus, Escherichia coli, and Prophyromonas gingivalis to determine the antimicrobial activity of leaf extracts of SG using agar well diffusion and broth dilution method. Results: ZOI was exhibited by ethanol extract (ESG) on S. mutans (25 ± 0.03 mm) and L. acidophilus (23 ± 0.07 mm) at 1 mg/mL while ZOI was exhibited by aqueous extract (ASG) on E. coli (14 ± 0.00 mm) and S. aureus (15 ± 0.01 mm) at 1.5 mg/mL. Both ESG and ASG did not show activity on P. gingivalis. MIC was obtained at 0.625 mg/mL for S. mutans, 0.312 mg/mL for L. acidophilus, 1.25 mg/mL for S. aureus, 0.625 mg/mL for E. coli, and P. gingivalis did not show inhibitory effect. Conclusion: The in vitro studies on antimicrobial activity showed antimicrobial activity of SG plant extract on oral microorganisms. This could be because of secondary metabolites like flavonoids, phenolics, etc.
引言:由生物膜引起的口腔疾病仍然是全世界关注的公共卫生问题。有趣的任务是与口腔生物膜“战斗”,主要是因为它们即使在机械去除后也会持续存在。机械口腔卫生措施,以及专业的氟化物维护和使用,是预防口腔生物膜的保守做法。建议在机械牙菌斑控制方法的基础上,使用抗菌漱口液。尽管氯己定是一种金标准的抗寄生虫剂,但其长期使用的潜在缺点需要开发一种新的、可抑制口腔生物膜的替代策略。材料与方法:采用浸渍法和索氏提取法制备白茅叶提取物。采用琼脂扩散法和肉汤稀释法测定SG叶提取物对变形链球菌、嗜酸乳杆菌、金黄色葡萄球菌、大肠杆菌和牙龈原单胞菌的抑菌活性。结果:乙醇提取物(ESG)对变形链球菌(25 ± 0.03 mm)和嗜酸乳杆菌(23 ± 0.07 mm) mg/mL,而水提取物(ASG)在大肠杆菌上表现出ZOI(14 ± 0 mm)和金黄色葡萄球菌(15 ± 0.01 mm) mg/mL。ESG和ASG均未显示对牙龈卟啉单胞菌的活性。MIC为0.625 变异链球菌,mg/mL,0.312 嗜酸乳杆菌,mg/mL,1.25 金黄色葡萄球菌,mg/mL,0.625 mg/mL对大肠杆菌和牙龈卟啉单胞菌没有显示出抑制作用。结论:SG植物提取物对口腔微生物具有抗菌活性。这可能是因为次生代谢产物,如类黄酮、酚类物质等。
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引用次数: 0
Comparative Evaluation of Teeth Displacement and Stress Generated with Orthodontic Mini-Implant and Infra-Zygomatic Crestal Implant during Intrusion in the Maxillary Arch − A Three Dimensional Finite Element Analysis 正畸微型种植体与颧下嵴种植体在上颌弓内嵌过程中牙位移与应力的比较分析——三维有限元分析
Q3 Dentistry Pub Date : 2023-01-01 DOI: 10.4103/jofs.jofs_151_23
Aarti Sethia, Anand Badavannavar, R. Hattarki, Tejashri Pradhan, Trupti Sadhunavar
Introduction: This study aims to evaluate and compare the stress distribution in bone and sutures surrounding the mini-implant and the teeth displacement pattern in the maxillary arch during full arch intrusion using a mini-implant, IZC implant, and an IZC implant with a mini-implant. Materials and Methods: Three individual finite element models of the craniofacial complex were generated for full arch intrusion with mini-implants and IZC implants using ANSYS 12.1 software. For group 1, five titanium mini-implants of 1.5 × 8 mm and 1.5 × 6 mm were placed 14 mm above the occlusal plane between the second premolar and the first molar, lateral incisors, canine, and between two central incisors, respectively, with forces application of 150 g posteriorly and 80 g anteriorly using a NiTi coil spring. For group 2, two stainless steel IZC implants of 2 × 14 mm were placed 16 mm above the occlusal plane between the first and second molars with a force application of about 300 g using a NiTi coil spring. In group 3, the placement of the IZC implant was similar to that in group 2, with an additional anterior mini-implant of 1.5 × 6 mm between two central incisors. An evaluation of the stress distribution and tooth displacement was carried out. Results: An increased amount of teeth displacement was observed in group 3 (IZC implant with mini-implants). Significant anterior intrusion was achieved in group 1 (mini-implants) whereas anteriors in group 2 (only IZC implants) experienced extrusion. A high amount of stress was observed in group 2. Conclusion: Therefore, IZC implants can be useful in cases of vertical maxillary excess where full arch intrusion is recommended. The anterior mini-implant helps to counteract the unwanted movement (extrusion) caused by the clockwise rotation of the maxilla. High stress levels are associated with an IZC implant without an anterior min-implant but they are within the confines of the physiologic limit of the bone.
简介:本研究旨在评估和比较迷你种植体、IZC种植体和IZC种植体与迷你种植体在全弓入侵时,颌骨周围骨和缝合线的应力分布和牙齿的位移模式。材料与方法:采用ANSYS 12.1软件对mini-种植体和IZC种植体进行全弓侵入,分别建立三个独立的颅面复合体有限元模型。组1:在第二前磨牙与第一磨牙之间、侧切牙之间、犬齿之间和两中切牙之间的咬合平面上方14mm处分别放置5个1.5 × 8mm和1.5 × 6mm的钛微型种植体,使用NiTi线圈弹簧前后分别施加150g和80g的力。在第二组,使用镍钛线圈弹簧将2颗2 × 14 mm的不锈钢IZC种植体放置在第一和第二磨牙之间的咬合平面上方16 mm处,施加约300 g的力。在3组中,IZC种植体的放置位置与2组相似,在两中切牙之间增加了1.5 × 6 mm的前牙微型种植体。对应力分布和齿位移进行了评估。结果:3组(IZC种植体+微型种植体)牙位移量增加。1组(微型种植体)实现了明显的前压,而2组(仅IZC种植体)的前压出现了挤压。第2组大鼠处于高应激状态。结论:IZC种植体在上颌垂直过度的情况下是有用的,建议全弓侵入。前牙微型种植体有助于抵消由上颌骨顺时针旋转引起的不必要的运动(挤压)。高应力水平与没有前牙种植体的IZC种植体有关,但它们在骨的生理极限范围内。
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引用次数: 0
Identification of heterochromatic variations in nonsyndromic cleft lip and palate 非综合征性唇腭裂异色变异的鉴定
Q3 Dentistry Pub Date : 2023-01-01 DOI: 10.4103/jofs.jofs_136_23
Soumya Raj, Leyon Varghese, Puthucode Narayanan, Suresh Raveendran, Pulikkottil Varghese, Alex George
Introduction: Orofacial cleft (OFC) has been one of the major common congenital anomalies exhibiting prominent ramifications allied with the medical, social, psychological, and economic strands. Most OFC occurrences do not have additional features, so they are categorized as nonsyndromic. The classification of the aforesaid complication has been directed toward the following categories: cleft lip (CL) with cleft palate, isolated CL, and finally the isolated cleft palate. The recent research concerning the aforementioned anomalies always searches for advanced novel inferences linked with the chromosomal perspectives since some of the specific genes are probably known to produce significant effects over the anomalies. Materials and Methods: Karyotyping was performed for all 130 cases of nonsyndromic cleft lip and palate (NSCLP). Aseptic collection of peripheral blood lymphocyte culture (PBLC) was performed from the patients using heparin vacutainers, and C-banding was done to confirm heterochromatic variations. Results: A total of 130 patients known to have the NSCLP were recruited for this study of which 88 cases (68%) had CL along with cleft palate, 18 cases (14%) had isolated CL and 24 cases (18%) had isolated cleft palate. Cytogenetic analysis by G-banding by Trypsin and Giemsa (GTG) banding in these patients revealed five cases (3.84%) with abnormal karyotype where a higher frequency of pericentric inversion in the analyzed region, specifically the chromosome 9, inv(9)(p11p13) was observed. Conclusion: The heteromorphisms or structural rearrangements involving the centromere were confirmed by centromere banding in two cases. Understanding the etiology with special inference on the above-said perspectives is significant to develop an effective strategy for the prevention and treatment of the individuals affected with the anomalies.
前言:口面裂(OFC)是一种常见的先天性畸形,在医学、社会、心理和经济等方面表现出突出的影响。大多数OFC没有其他特征,因此它们被归类为非综合征性。上述并发症的分类可分为以下几类:唇裂合并腭裂,孤立性唇裂,最后是孤立性腭裂。由于已知某些特定基因可能对上述异常产生重大影响,因此最近对上述异常的研究总是寻求与染色体角度相关的先进的新推断。材料与方法:对130例非综合征性唇腭裂(NSCLP)患者进行染色体核型分析。使用肝素真空瓶无菌收集患者外周血淋巴细胞培养(PBLC),并进行c带分析以确认异色变异。结果:本研究共招募了130例已知患有NSCLP的患者,其中88例(68%)患有CL合并腭裂,18例(14%)患有孤立性CL, 24例(18%)患有孤立性腭裂。对这些患者进行胰蛋白酶和吉姆萨(GTG) g带带细胞遗传学分析,发现5例(3.84%)核型异常,在分析区域,特别是9号染色体inv(9)(p11p13)中观察到较高频率的中心周围反转。结论:两例着丝粒带带证实了着丝粒的异型性或结构重排。了解病因与上述观点的特殊推断,对于制定有效的策略来预防和治疗受异常影响的个体具有重要意义。
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引用次数: 0
OCT4 Positive Cancer Stem Cell Population in Oral Carcinogenesis OCT4阳性肿瘤干细胞在口腔癌发生中的作用
Q3 Dentistry Pub Date : 2023-01-01 DOI: 10.4103/jofs.jofs_163_23
Varun Pillai, Prathiba Ramani, Jayanthi Palani
Introduction: Oral squamous cell carcinoma (OSCC) is the predominant histological subtype of oral cancer, which is the sixth most common malignancy worldwide. Despite the advances in therapy, the overall survival rate of oral cancer ranges between 45% and 50%. Cancer stem cells (CSCs) are a small subset of cancer cells that are believed to contribute to local recurrence and therapeutic resistance in OSCC. Cancer stem cells in OSCC express many of the same proteins involved in the core network that regulates embryonic stem cells (ESCs) such as NANOG, OCT4, and SOX2. Octomer binding transcription factor 4 (OCT4) is considered to be one of the major regulators for self-renewal and the maintenance of the stem cell population in the undifferentiated tissue. This study was done to evaluate the expression for OCT4 in OSCC and oral epithelial dysplasia (OED) using immunohistochemistry. Materials and Methods: Histologically proven 40 cases of OSCC, 40 cases of oral leukoplakia with epithelial dysplasia, and 25 cases of normal oral mucosa (NOM) were assessed for immunohistochemical expression of OCT4. The percentage positivity and mean expression of OCT4 were calculated. The final immunohistochemical score was obtained by adding the mean expression and staining intensity of OCT4. Results: The mean expression of OCT4 in OSCC, OED, and NOM was 3.85± 1.05, 8.64± 2.12, and 1.75± 0.23 and the difference was statistically significant (P < 0.01). A higher expression score of OCT4 was observed in 8% and 12% of OSCC and OED, respectively. Conclusion: The higher expression of OCT4 in OSCC and OED suggests that in addition to playing a role in tumorigenesis, OCT4 might be a potential marker for malignant transformation in OED.
简介:口腔鳞状细胞癌(OSCC)是口腔癌症的主要组织学亚型,是全球第六常见的恶性肿瘤。尽管治疗取得了进展,但口腔癌症的总生存率在45%至50%之间。癌症干细胞(CSCs)是癌症细胞的一小部分,被认为有助于OSCC的局部复发和治疗耐药性。OSCC中的癌症干细胞表达许多与调节胚胎干细胞(ESC)的核心网络(如NANOG、OCT4和SOX2)有关的相同蛋白质。八聚体结合转录因子4(OCT4)被认为是未分化组织中自我更新和维持干细胞群的主要调节因子之一。本研究采用免疫组织化学方法评估OCT4在口腔鳞状细胞癌和口腔上皮发育不良(OED)中的表达。材料和方法:对40例经组织学证实的OSCC、40例伴有上皮发育不良的口腔白斑和25例正常口腔粘膜(NOM)进行OCT4的免疫组织化学表达评估。计算OCT4的阳性率和平均表达。通过添加OCT4的平均表达和染色强度来获得最终的免疫组织化学评分。结果:OCT4在OSCC、OED和NOM中的平均表达为3.85± 1.058.64± 2.12和1.75± 0.23,差异有统计学意义(P<0.01)。OCT4在OSCC和OED中的表达得分分别为8%和12%。结论:OCT4在OSCC和OED中的高表达表明,OCT4除了在肿瘤发生中发挥作用外,还可能是OED恶性转化的潜在标志物。
{"title":"OCT4 Positive Cancer Stem Cell Population in Oral Carcinogenesis","authors":"Varun Pillai, Prathiba Ramani, Jayanthi Palani","doi":"10.4103/jofs.jofs_163_23","DOIUrl":"https://doi.org/10.4103/jofs.jofs_163_23","url":null,"abstract":"Introduction: Oral squamous cell carcinoma (OSCC) is the predominant histological subtype of oral cancer, which is the sixth most common malignancy worldwide. Despite the advances in therapy, the overall survival rate of oral cancer ranges between 45% and 50%. Cancer stem cells (CSCs) are a small subset of cancer cells that are believed to contribute to local recurrence and therapeutic resistance in OSCC. Cancer stem cells in OSCC express many of the same proteins involved in the core network that regulates embryonic stem cells (ESCs) such as NANOG, OCT4, and SOX2. Octomer binding transcription factor 4 (OCT4) is considered to be one of the major regulators for self-renewal and the maintenance of the stem cell population in the undifferentiated tissue. This study was done to evaluate the expression for OCT4 in OSCC and oral epithelial dysplasia (OED) using immunohistochemistry. Materials and Methods: Histologically proven 40 cases of OSCC, 40 cases of oral leukoplakia with epithelial dysplasia, and 25 cases of normal oral mucosa (NOM) were assessed for immunohistochemical expression of OCT4. The percentage positivity and mean expression of OCT4 were calculated. The final immunohistochemical score was obtained by adding the mean expression and staining intensity of OCT4. Results: The mean expression of OCT4 in OSCC, OED, and NOM was 3.85± 1.05, 8.64± 2.12, and 1.75± 0.23 and the difference was statistically significant (P < 0.01). A higher expression score of OCT4 was observed in 8% and 12% of OSCC and OED, respectively. Conclusion: The higher expression of OCT4 in OSCC and OED suggests that in addition to playing a role in tumorigenesis, OCT4 might be a potential marker for malignant transformation in OED.","PeriodicalId":16651,"journal":{"name":"Journal of Orofacial Sciences","volume":"15 1","pages":"86 - 91"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48124495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expression of fibroblast activation protein alpha in odontogenic keratocyst and ameloblastoma: A cross-sectional immunohistochemical study 成纤维细胞活化蛋白α在牙源性角化囊肿和成釉细胞瘤中的表达:横断面免疫组织化学研究
Q3 Dentistry Pub Date : 2023-01-01 DOI: 10.4103/jofs.jofs_17_23
Sandhya Tamgadge, T. Pereira
Introduction: Cancer has always been a mystery for the researcher, healthcare providers, and even patients. This could be because of the unexplored journey of a cell from its physiological to cancerous form. Every day, new research articles are being emerged on various platforms where researchers have been attempting to explore the hidden signals of carcinogenesis. Cancer-associated fibroblasts (CAFs) are one of the key proteins. Fibroblast activation protein alpha (FAPα) is found in normal-appearing surrounding tumor microenvironments (TMEs) and shows a strong positive correlation with high tumor grade. It has been found exceedingly expressive in oral squamous cell carcinoma. Similarly, odontogenic lesions too are highly destructive and show a high recurrence rate. Thus, there is a crucial need to assess FAPα in these lesions too. This article is a preliminary attempt to evaluate FAPα expression in ameloblastoma and odontogenic keratocyst, which are highly destructive lesions of the jaws. Materials and Methods: The study group comprised 40 cases each of odontogenic keratocyst (OKC) and ameloblastoma . A total of 10 cases each of lymphoma (5 negative control) and colorectal carcinoma (5 positive control) were selected as control. Both groups were immunohistochemically stained using FAPα antibody. The study group was compared with clinical parameters and analyzed statistically using chi-square tests to find out correlation, and phi coefficient and Cramer V test were used to test the strength of association. Kendall coefficient of rank correlation tau-sub-b (τb) was used to correlate the final immunoreactivity score (IRS) and the age and dimension of the lesion. Kappa correlation was calculated to assess interobserver variability. Results: There was a significant correlation between the extension of the lesion and the FAPα of each group. Other correlations showed insignificant correlations. But both groups showed more cases in moderate and very strong IRS. Conclusion: There is a strong correlation between the FAP expression and extension of the lesion in OKC and ameloblastoma. There was a strong role for FAPα in the pathogenesis of OKC and ameloblastoma.
简介:癌症一直是研究人员、医疗保健提供者甚至患者的一个谜。这可能是因为细胞从生理形态到癌变的过程尚未探索。每天,研究人员都在各种平台上发表新的研究文章,试图探索致癌的隐藏信号。癌相关成纤维细胞(CAFs)是关键蛋白之一。成纤维细胞活化蛋白α(FAPα)存在于正常出现的肿瘤周围微环境(TMEs)中,并与高肿瘤分级呈正相关。它已被发现在口腔鳞状细胞癌中极为表达。同样,牙源性病变的破坏性也很强,复发率也很高。因此,评估这些病变中的FAPα也是至关重要的。本文初步尝试评估FAPα在成釉细胞瘤和牙源性角化囊肿中的表达,这两种病变是颌骨的高度破坏性病变。材料和方法:研究组包括40例牙源性角化囊肿(OKC)和成釉细胞瘤。选择淋巴瘤(5例阴性对照)和结直肠癌(5例阳性对照)各10例作为对照。两组均用FAPα抗体进行免疫组化染色。将研究组与临床参数进行比较,并使用卡方检验进行统计学分析以找出相关性,使用phi系数和Cramer V检验来检验关联强度。Kendall秩相关系数tau-sub-b(τb)用于将最终免疫反应性评分(IRS)与病变的年龄和尺寸相关联。计算Kappa相关性以评估观察者间的变异性。结果:各组的FAPα与病变范围有显著相关性。其他相关性显示出不显著的相关性。但这两组都显示出中度和非常强烈IRS的病例更多。结论:FAP在OKC和成釉细胞瘤中的表达与病变范围密切相关。FAPα在OKC和成釉细胞瘤的发病机制中具有重要作用。
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引用次数: 0
Extracellular microRNA-223-5p Levels in Plasma, Saliva, and Gingival Crevicular Fluid in Periodontal Disease as a Potential Diagnostic Marker − A Case–Control Analysis 牙周病患者血浆、唾液和龈沟液中细胞外微小RNA-223-5p水平作为潜在的诊断标志物——病例对照分析
Q3 Dentistry Pub Date : 2023-01-01 DOI: 10.4103/jofs.jofs_177_23
D. Bandi, Uma Sudhakar, Harinath Parthasarathy, Snophia Rajamani, Balasubramanian Krishnaswamy
Introduction: Periodontal disease is distinguished by an aberrant host response to oral pathogens, leading to soft and hard tooth-supporting tissue inflammation. MicroRNAs are minute, single-stranded, highly dynamic biomolecules that control gene expression and regulate protein synthesis and functioning. Periodontal pathogenesis is associated with microRNA dysregulation. Accordingly, the proposed study will evaluate the extracellular circulating microRNA-223-5p in the plasma, saliva, and gingival crevicular fluid (GCF) of patients with periodontal disease. Materials and Methods: The research population comprised of 50 healthy individuals and 50 periodontitis patients. The clinical parameters of each participant were documented. Under sterile conditions, blood, saliva, and GCF were collected and stored at −80 °C. MicroRNA was isolated using microRNA extraction kits in accordance with the manufacturer’s instructions, and the expression pattern of mir-223-5p in body fluids was analyzed using real-time polymerase chain reaction. Results: Expression of circulating extracellular microRNA-223-5p is elevated (P = 0.05) in plasma, saliva, and GCF by a fold of 2.511, 8.072, and 10.46, respectively. The clinical parameters, clinical attachment loss, and probing pocket depth correlated significantly and positively with an increase in miR-223-5p expression (P = 0.05). According to a ROC analysis, MicroRNA-223-5p may be a viable biomarker for periodontal disease, with a diagnostic accuracy of 84.50%. Conclusion: In conclusion, extracellular microRNA-223-5p detected in plasma, saliva, and GCF can be a reliable biomarker for periodontal disease. GCF is a potential body fluid for the analysis of microRNA-223 in relation to periodontal disease considering its expression is significantly higher in comparison to that of plasma and saliva. Due to their exceptional stability in body fluids, extracellular microRNAs can be employed as periodontal disease detectors, forecasting variables of treatment, and for the tailored modalities of treatment.
简介:牙周病的特点是宿主对口腔病原体的异常反应,导致牙齿软硬组织炎症。微小核糖核酸是微小的、单链的、高度动态的生物分子,控制基因表达并调节蛋白质合成和功能。牙周病的发病机制与微小核糖核酸失调有关。因此,拟议的研究将评估牙周病患者血浆、唾液和龈沟液(GCF)中的细胞外循环微小RNA-223-5p。材料和方法:研究人群包括50名健康人和50名牙周炎患者。记录每个参与者的临床参数。在无菌条件下,收集血液、唾液和GCF,并将其储存在−80 °C。根据制造商的说明,使用微小RNA提取试剂盒分离微小RNA,并使用实时聚合酶链式反应分析体液中mir-223-5p的表达模式。结果:循环细胞外微小RNA-223-5p表达升高(P = 0.05)分别增加了2.511、8.072和10.46倍。临床参数、临床附着丧失和探测袋深度与miR-223-5p表达的增加显著正相关(P = 0.05)。根据ROC分析,微小RNA-223-5p可能是牙周病的一种可行的生物标志物,诊断准确率为84.50%。结论:总之,在血浆、唾液和GCF中检测到的细胞外微小RNA-223-5 p可能是一种可靠的牙周病生物标志物。GCF是用于分析与牙周病相关的微小RNA-223的潜在体液,因为与血浆和唾液相比,其表达显著更高。由于其在体液中的特殊稳定性,细胞外微小RNA可以用作牙周病检测器,预测治疗变量,并用于量身定制的治疗模式。
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引用次数: 0
Evaluation of Bromelain, A Pineapple Extract as a Bleaching Agent on Human Enamel and Determining its Concentration by Reverse-HPLC Method 菠萝提取物菠萝蛋白酶对人牙釉质漂白作用的评价及反高效液相色谱法测定其浓度
Q3 Dentistry Pub Date : 2023-01-01 DOI: 10.4103/jofs.jofs_116_23
Ramya Sheshadri, A. Shivakumar, Bhaskaran Mahendran, Ravandur Chandan, S. Kalgeri
Introduction: In recent times, there has been an increase in the number of esthetic smile makeovers. As a part of these procedures, achieving a brighter smile is considered essential. The treatment options for such makeovers range from simple bleaching to complex restorations. Bleaching, a conservative and straightforward method to manage discolored teeth, has become popular. Therefore, vital bleaching has gained immense popularity. To evaluate and compare the color changes on human enamel bleached with different concentrations of hydrogen peroxide, containing pineapple extract as an additive, and determine the bromelain content in pineapple, by reversed-phase ‑ HPLC method. Material and Methods: Twenty permanent single-rooted artificially stained maxillary anterior teeth were decoronated at CEJ, the crown component was divided into two vertical halves, further divided into four groups containing 10 samples each and bleached accordingly. Group I (A) 15% H2O2 only, Group I (B) 2 mL of pineapple extract and 28 mL of 15% H2O2. Group II (A) 25% H2O2 only and Group II (B) 2 mL of pineapple extract and 28 mL of 25% H2O2. The samples were checked for color change with the help of a reflectance spectrophotometer. RP-HPLC method was used to find the proportion of bromelain in pineapple extract. Statistical analysis: carried out using one-way ANOVA for comparison between two groups and Scheffe’s post hoc test for comparison between more than two groups. “Significance level” for all statistical tests was set at P < 0.05 (significance level <5%). All these analyses were carried out using the SPSS Version 22 software. Results: Group II B showed the maximum change in color means ΔE compared to the other three groups. The mean color change ΔE between Group II A and ΔE Group I B showed no statistical difference. The mean color change ΔE of Group I A showed the least color change compared to all other groups. Group II B showed a statistically significant change in color with “P −0.001” compared to all other groups. Linearity overlay on chromatogram by RP-HPLC method showed the concentration of bromelain in pineapple extract is 64.99 mcg/mL. Conclusion: Pineapple extracts along with hydrogen peroxide showed promising results with a new gateway of success in whitening teeth.
简介:近年来,美容微笑的次数有所增加。作为这些程序的一部分,获得更灿烂的笑容被认为是必不可少的。这种修复的治疗选择从简单的漂白到复杂的修复。漂白,一种保守而直接的方法来处理变色的牙齿,已经变得流行起来。因此,活性漂白获得了极大的普及。以菠萝提取物为添加剂,评价和比较不同浓度过氧化氢漂白人牙釉质的颜色变化,并采用反相高效液相色谱法测定菠萝中菠萝蛋白酶的含量。材料和方法:在CEJ对20颗永久性单根人工染色上颌前牙进行去角质,将牙冠部分垂直分为两半,再分为四组,每组10个样本,并进行相应的漂白。I组(A)仅15%H2O2,I组(B)2 mL菠萝提取物和28 mL的15%H2O2。第II(A)组仅含25%H2O2,第II(B)组2 mL菠萝提取物和28 mL的25%H2O2。借助反射分光光度计检查样品的颜色变化。采用反相高效液相色谱法测定菠萝提取物中菠萝蛋白酶的含量。统计分析:使用单因素方差分析进行两组之间的比较,使用Scheffe的事后检验进行两组以上的比较。所有统计检验的“显著性水平”均设定为P<0.05(显著性水平<5%)。所有这些分析均使用SPSS Version 22软件进行。结果:与其他三组相比,II B组的颜色均值ΔE变化最大。ⅡA组和ⅠB组的平均颜色变化ΔE无统计学差异。与所有其他组相比,I A组的平均颜色变化ΔE显示出最小的颜色变化。与所有其他组相比,II B组的颜色变化具有统计学意义,“P−0.001”。反相高效液相色谱法测定菠萝蛋白酶在菠萝提取物中的浓度为64.99 mcg/mL。结论:菠萝提取物和过氧化氢在美白牙齿方面显示出良好的效果,为美白牙齿开辟了新的成功途径。
{"title":"Evaluation of Bromelain, A Pineapple Extract as a Bleaching Agent on Human Enamel and Determining its Concentration by Reverse-HPLC Method","authors":"Ramya Sheshadri, A. Shivakumar, Bhaskaran Mahendran, Ravandur Chandan, S. Kalgeri","doi":"10.4103/jofs.jofs_116_23","DOIUrl":"https://doi.org/10.4103/jofs.jofs_116_23","url":null,"abstract":"Introduction: In recent times, there has been an increase in the number of esthetic smile makeovers. As a part of these procedures, achieving a brighter smile is considered essential. The treatment options for such makeovers range from simple bleaching to complex restorations. Bleaching, a conservative and straightforward method to manage discolored teeth, has become popular. Therefore, vital bleaching has gained immense popularity. To evaluate and compare the color changes on human enamel bleached with different concentrations of hydrogen peroxide, containing pineapple extract as an additive, and determine the bromelain content in pineapple, by reversed-phase ‑ HPLC method. Material and Methods: Twenty permanent single-rooted artificially stained maxillary anterior teeth were decoronated at CEJ, the crown component was divided into two vertical halves, further divided into four groups containing 10 samples each and bleached accordingly. Group I (A) 15% H2O2 only, Group I (B) 2 mL of pineapple extract and 28 mL of 15% H2O2. Group II (A) 25% H2O2 only and Group II (B) 2 mL of pineapple extract and 28 mL of 25% H2O2. The samples were checked for color change with the help of a reflectance spectrophotometer. RP-HPLC method was used to find the proportion of bromelain in pineapple extract. Statistical analysis: carried out using one-way ANOVA for comparison between two groups and Scheffe’s post hoc test for comparison between more than two groups. “Significance level” for all statistical tests was set at P < 0.05 (significance level <5%). All these analyses were carried out using the SPSS Version 22 software. Results: Group II B showed the maximum change in color means ΔE compared to the other three groups. The mean color change ΔE between Group II A and ΔE Group I B showed no statistical difference. The mean color change ΔE of Group I A showed the least color change compared to all other groups. Group II B showed a statistically significant change in color with “P −0.001” compared to all other groups. Linearity overlay on chromatogram by RP-HPLC method showed the concentration of bromelain in pineapple extract is 64.99 mcg/mL. Conclusion: Pineapple extracts along with hydrogen peroxide showed promising results with a new gateway of success in whitening teeth.","PeriodicalId":16651,"journal":{"name":"Journal of Orofacial Sciences","volume":"15 1","pages":"31 - 37"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42187351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative evaluation of antibacterial efficacy of chlorhexidine mouthwash and Momordica charantia, Spinacia oleracea mouthwash against Streptococcus mutans, Lactobacillus spp., and Porphyromonas gingivalis − An in vitro study 氯己定漱口水与苦瓜、菠菜漱口水对变形链球菌、乳酸杆菌和牙龈卟啉单胞菌抗菌效果的比较评价——一项体外研究
Q3 Dentistry Pub Date : 2023-01-01 DOI: 10.4103/jofs.jofs_115_23
Krishna S Kadam, Niraj S. Gokhale, S. Hugar, Neha Kohli, Suneel S. Dodamani, S. Tendulkar
Introduction: Various advances have been made in pharmacology and synthetic organic chemistry, the dependency on natural products, particularly on herbs, remains relatively unchanged. Among various herbal products that are being used in dentistry, efficacy of mouthwash prepared using Momordica charantia extract and Spinacia oleracea extract on prevention of caries in children has not been researched yet. Hence, in this study the antibacterial efficacy of chlorhexidine mouthwash was evaluated and compared with Momordica charantia, Spinacia oleracea mouthwash against Streptococcus mutans and Lactobacillus acidophilus, Porphyromonas gingivalis. To evaluate and compare the antimicrobial efficacy of chlorhexidine mouthwash and Momordica charantia, Spinacia oleracea mouthwash against Streptococcus mutans, Lactobacillus acidophilus, and Porphyromonas gingivalis. Materials and Methods: Ethanolic extract of Momordica charantia and Spinacia oleracea was prepared. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of these extracts were then determined against Streptococcus mutans, Lactobacillus acidophilus, and Porphyromonas gingivalis using resazurin method and agar plate streaking method. Herbal mouthwashes were then prepared from the extracts using MIC and MBC values and its cytotoxicity was determined using MTT assay. Antibacterial susceptibility was then determined using agar well diffusion method and time-kill assay. Results: There is no statistically significant difference in the effectiveness of chlorhexidine mouthwash and Momordica charantia, Spinacia oleracea mouthwash against Streptococcus mutans, Lactobacillus acidophilus, and Porphyromonas gingivalis. Conclusion: Momordica charantia extract mouthwash and Spinacia oleracea extract mouthwash can be used as an herbal alternative as it has equal antibacterial efficacy against Streptococcus mutans, Lactobacillus acidophilus, Porphyromonas gingivalis as compared to 0.2% chlorhexidine mouthwash.
引言:药理学和合成有机化学取得了各种进展,对天然产物的依赖,特别是对草药的依赖,相对没有变化。在牙科使用的各种草药产品中,使用苦瓜提取物和菠菜提取物制备的漱口水预防儿童龋齿的功效尚未得到研究。因此,本研究评估了氯己定漱口水对变形链球菌和嗜酸乳杆菌、牙龈卟啉单胞菌的抗菌效果,并与苦瓜、菠菜漱口水进行了比较。评价和比较氯己定漱口水与苦瓜、菠菜漱口水对变形链球菌、嗜酸乳杆菌和牙龈卟啉单胞菌的抗菌效果。材料与方法:制备苦瓜和菠菜乙醇提取物。然后使用雷萨祖林法和琼脂平板划线法测定这些提取物对变形链球菌、嗜酸乳杆菌和牙龈卟啉单胞菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。然后用MIC和MBC值从提取物中制备草药漱口水,并用MTT法测定其细胞毒性。然后用琼脂扩散法和时间杀伤法测定抗菌药物的敏感性。结果:洗必泰漱口水与苦瓜、菠菜漱口水对变形链球菌、嗜酸乳杆菌和牙龈卟啉单胞菌的疗效无统计学差异。结论:苦瓜提取物漱口水和菠菜提取物漱口水对变形链球菌、嗜酸乳杆菌、牙龈卟啉单胞菌的抗菌效果与0.2%氯己定漱口水相当,可作为草药替代品。
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引用次数: 0
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Journal of Orofacial Sciences
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