Phytochemical Screening and HPTLC Analysis of Bio-active Markers of Ethanol Extract of Indian Bay Leaves

Abstract

ABSTRACT Solvent extracts of Cinnamomum tamala (CT) leaves were screened for phytochemicals, and the flavonoid and phenolic contents were estimated. The two standard marker compounds quercetin and eugenol were also quantified using high-performance thin-layer chromatography (HPTLC). The total flavonoid and phenolic contents were determined using aluminum chloride and Folin–Ciocalteu colorimetric methods, respectively. The ethanolic extracts had the highest total flavonoid and phenolic contents with 141.31 ± 0.053 mg QE g−1 and 178.96 ± 0.62 mg GAE g−1, respectively. The HPTLC method was used to detect spots for quercetin and eugenol (Rf value of 0.63 and 0.59, respectively). The amounts of eugenol and quercetin were 452.6 µg and 705.6 µg in 100 mg of the ethanolic extract of CT leaves, respectively.