Antibacterial Activity Test of Herbal Liquid Soap Against Streptoccus pyogenes and Candida albicans

Darini Kurniawati
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Abstract

Nowadays people like to use liquid soap because it is more practical and easier to store. Antiseptic liquid soap circulating in the market uses chemical compounds such as triclosan, hexalorofen and bithional whose raw materials are from abroad. The government encourages the use of its own natural raw materials so that natural materials will become the mainstay of Indonesian industry. The purpose of this research was knowing the antibacterial effectiveness of herbal liquid soap against Streptococcus pyogenes and Candida albicans. This research used true experimental method which the process was started by making herbal liquid soap products using the hot; and cold press method for evaluating pH, organoleptic, foam stability, water content, free fatty acids and free alkali, testing germ numbers with the intervention group (washing hands with herbal liquid soap). and control group (washing hands with running water), followed by testing the inhibitory power of herbal liquid soap against Streptococcus pyogenes and Candida albican microbes. The results of the evaluation test of herbal liquid soap have a liquid form, lemon odor and yellow color, foam height 15-70 mm, pH 10, water content 52%, free fatty acids 0.05%, free alkali 0.091%, according to SNI soap standards. required liquid. Antibacterial test by dilution method and continued with microbial inhibition results obtained MIC against Streptococcus pyogenes at a concentration of 20% and MBC at a concentration of 20% of 22mm. Against Candida albican, the results of MIC at a concentration of 25% and MBC at a concentration of 25% were 15 mm.
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草药液皂对化脓性链球菌和白色念珠菌的抑菌活性试验
现在人们喜欢用液体肥皂,因为它更实用,更容易储存。市面上流通的消毒液皂使用的三氯生、六氯芬、双硫通等化合物的原料均来自国外。政府鼓励使用本国的天然原材料,使天然材料成为印尼工业的支柱。本研究的目的是了解草药液体皂对化脓性链球菌和白色念珠菌的抗菌效果。本研究采用真正的实验方法,首先利用高温制作草药皂液产品;冷压法评价pH值、感官、泡沫稳定性、含水量、游离脂肪酸和游离碱,用干预组(用草药液皂洗手)检测细菌数量。对照组(用自来水洗手),然后测试草药液体皂对化脓性链球菌和白色念珠菌的抑菌能力。评价试验结果草药液皂呈液体状,有柠檬气味,颜色呈黄色,泡沫高度15-70 mm, pH值10,含水量52%,游离脂肪酸0.05%,游离碱0.091%,符合SNI皂标准。所需的液体。用稀释法进行抑菌试验,继续进行微生物抑制实验,得到对化脓性链球菌的MIC浓度为20%,MBC浓度为20% (22mm)。MIC浓度为25%,MBC浓度为25%,对白色念珠菌的杀伤效果为15 mm。
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