Comparative transcriptome analysis reveals potential gene targets for ovipositional inhibition by diallyl trisulfide against female Sitotroga cerealella

Q1 Agricultural and Biological Sciences Agri Gene Pub Date : 2017-09-01 DOI:10.1016/j.aggene.2017.07.001
Min Ma , Yi-Yi Ying , Zhi-Ya He , Yan Lu , Xue-Gang Li , Chao-Liang Lei , Feng-Lian Yang
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引用次数: 2

Abstract

The angoumois grain moth, Sitotroga cerealella Olivier, is a serious stored-grain pest worldwide. In previous studies, we demonstrated that diallyl trisulfide (DATS), one of active substances from garlic essential oil, inhibited oviposition of S. cerealella, but the mechanism for this inhibition remains unclear. We suspected that DATS-regulated gene expression changes in S. cerealella may be a reason for the modulation of fertility and reproductive behaviors of the moth. Comparative transcriptomes of female S. cerealella were built by RNA-sequencing to detect differences between DATS exposure and background air with special lights on the key genes related to the fecundity. A total of 304 differentially expressed genes (DEGs) were detected in female moth after exposure to DATS, of which 120 were up-regulated, and 184 were down-regulated. Sixty-two of these DEGs encoding proteins involved in growth and development of eggs and regulation of hormones and mating behavior were identified, and of these, 49 were annotated as chorion protein. Meanwhile, quantitative real-time PCR verified that DEG data were reliable for further transcriptional analysis. Additionally, the results of ovarian dissection and oviposition activity bioassay suggest that DATS has negative effects on ovarian development and fecundity of S. cerealella. Our comprehensive sequence resource with desirable quality enriched the genomic platform of S. cerealella, and the DEGs that were identified will facilitate understanding of the molecular mechanism of ovipositional inhibition by DATS.

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比较转录组分析揭示了三硫二烯丙基对雌性谷曲菌产卵抑制的潜在基因靶点
谷粒蛾(Sitotroga cerealella Olivier)是世界范围内严重的储粮害虫。在以往的研究中,我们发现大蒜精油中的活性物质之一二烯丙基三硫醚(diallyl trisulfide, DATS)可以抑制小谷杆菌的产卵,但其抑制机制尚不清楚。我们推测,dats调控的基因表达变化可能是小麦黑麦蛾生育和生殖行为调控的原因之一。利用rna测序技术构建了雌性麦谷菌的比较转录组,以检测DATS暴露与特殊光照背景空气中与繁力相关的关键基因的差异。暴露于DATS后,雌蛾共检测到304个差异表达基因,其中上调120个,下调184个。这些基因编码蛋白中有62个参与卵子的生长发育、调节激素和交配行为,其中49个被标记为绒毛膜蛋白。同时,实时荧光定量PCR验证了DEG数据的可靠性,可用于进一步的转录分析。此外,卵巢解剖和产卵活性生物测定结果表明,DATS对谷类小麦的卵巢发育和繁殖能力有负面影响。我们的完整的高质量序列资源丰富了谷类小麦的基因组平台,所鉴定的DEGs将有助于理解DATS对产卵抑制的分子机制。
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Agri Gene
Agri Gene Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
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期刊介绍: Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.
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