Cell Therapy and Investigation of Angiogenesis of Fibroblastswith Collagen Hydrogel on the Healing of Diabetic Wounds

IF 1.8 Q3 PHARMACOLOGY & PHARMACY Turkish Journal of Pharmaceutical Sciences Pub Date : 2022-12-28 DOI:10.4274/tjps.galenos.2022.62679
Abbas Zabihi, Sanaz Pashapour, M. Mahmoodi
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Abstract

INTRODUCTION: A diabetic ulcer is a common disease in diabetic patients. Due to antibiotic resistance, new therapeutic alternatives are being considered in diabetic foot patients to reduce complications and mortality. This study aimed to evaluate the effect of collagen hydrogel on wound healing process in diabetic rats. METHODS: Diabetic wounds were induced with streptozotocin in all 42 male Wistar rats. The rats were divided into four groups: (a) treated with fibroblast cells, (b) collagen hydrogel, (c) collagen cultured with fibroblast cells, and (d) control group. Microscopic and histological (H&E staining and Mason trichrome staining), measurement of wound surface with Image J, skin density and thickness by the ultrasound probe, and skin elasticity with cutometer tool was used to evaluate the wound healing in a days , 14, and 21 after the treatment. RESULTS: The results showed that the treatment of diabetic wounds with fibroblast cells cultured in collagen hydrogel greatly reduces inflammatory responses in the skin tissue and significantly accelerates the healing process. Also, 21 days after the start of treatment, skin elasticity, thickness and density were higher in the collagen + fibroblast group than in the control group.
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胶原水凝胶对成纤维细胞治疗及血管生成的研究
简介:糖尿病性溃疡是糖尿病患者的常见病。由于抗生素耐药性,新的治疗方案正在考虑糖尿病足患者,以减少并发症和死亡率。本研究旨在探讨胶原水凝胶对糖尿病大鼠创面愈合过程的影响。方法:采用链脲佐菌素诱导42只雄性Wistar大鼠糖尿病创面。将大鼠分为四组:(a)成纤维细胞组,(b)胶原水凝胶组,(c)成纤维细胞培养胶原组,(d)对照组。采用显微和组织学(H&E染色和Mason三色染色)、Image J创面测量、超声探头皮肤密度和厚度、刀具皮肤弹性评估治疗后1天、14天、21天创面愈合情况。结果:胶原水凝胶培养成纤维细胞治疗糖尿病创面可显著降低皮肤组织炎症反应,显著加快创面愈合进程。治疗21 d后,胶原+成纤维细胞组皮肤弹性、厚度和密度均高于对照组。
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CiteScore
3.60
自引率
5.90%
发文量
79
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