Pub Date : 2024-07-12DOI: 10.4274/tjps.galenos.2023.83451
Dina Manny, Amr El-Waseif, Eslam Abd El-Wahed
Objectives: Enterocin is a significant broad-spectrum peptide antibiotic produced by Enterococcus faecium (E. faecium). Enterocin production by E. faecium was investigated using the Taguchi experimental design. The Taguchi models were used to save the time and effort required for optimizing the different conditions affecting its production. They were applied to optimize the conditions for enterocin production using the least number of experiments and the least number of required materials.
Materials and methods: Seven factors i.e., pH, temperature, time of incubation, aeration rate, inoculum size, carbohydrate concentration, and bile salt concentrations, each at three levels were selected and an orthogonal array layout of L273 was performed.
Results: The experimental results indicated that the best incubation conditions were; 48 hours incubation on a nutrient medium at pH 6.5, temperature at 25 °C, aeration rate at 0 round per minute, inoculum size 20 mL, and bile salt concentration. It was 5%, and the carbon concentration was 2.0%. All these factors combined led to the best enterocin production by E. faecium.
Conclusion: This optimization of enterocin production by the Taguchi experimental models emphasized some important results regarding the interaction of the different driving factors leading to the best enterocin production in one experiment.
{"title":"Optimization of Enterocin Production from Probiotic <i>Enterococcus faecium</i> Using Taguchi Experimental Design.","authors":"Dina Manny, Amr El-Waseif, Eslam Abd El-Wahed","doi":"10.4274/tjps.galenos.2023.83451","DOIUrl":"10.4274/tjps.galenos.2023.83451","url":null,"abstract":"<p><strong>Objectives: </strong>Enterocin is a significant broad-spectrum peptide antibiotic produced by <i>Enterococcus faecium</i> (<i>E. faecium</i>). Enterocin production by <i>E. faecium</i> was investigated using the Taguchi experimental design. The Taguchi models were used to save the time and effort required for optimizing the different conditions affecting its production. They were applied to optimize the conditions for enterocin production using the least number of experiments and the least number of required materials.</p><p><strong>Materials and methods: </strong>Seven factors i.e., pH, temperature, time of incubation, aeration rate, inoculum size, carbohydrate concentration, and bile salt concentrations, each at three levels were selected and an orthogonal array layout of L27<sup>3</sup> was performed.</p><p><strong>Results: </strong>The experimental results indicated that the best incubation conditions were; 48 hours incubation on a nutrient medium at pH 6.5, temperature at 25 °C, aeration rate at 0 round per minute, inoculum size 20 mL, and bile salt concentration. It was 5%, and the carbon concentration was 2.0%. All these factors combined led to the best enterocin production by <i>E. faecium</i>.</p><p><strong>Conclusion: </strong>This optimization of enterocin production by the Taguchi experimental models emphasized some important results regarding the interaction of the different driving factors leading to the best enterocin production in one experiment.</p>","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"1 1","pages":"192-198"},"PeriodicalIF":1.8,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41613629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-25DOI: 10.4274/tjps.galenos.2023.72430
Uğur Karagöz, Remant Bahadur Kc, Elif İşel, A. G. Kantarci, Hasan Uludag
{"title":"Evaluation of a Synthetic PEI-based Polymeric Vector for ING4 Gene Delivery to MCF-7 Breast Cancer Cells","authors":"Uğur Karagöz, Remant Bahadur Kc, Elif İşel, A. G. Kantarci, Hasan Uludag","doi":"10.4274/tjps.galenos.2023.72430","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2023.72430","url":null,"abstract":"","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"31 4","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139157950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of Anticancer and Antioxidant Activities of Coffee Stem Parasite Extract (Scurrula Ferruginea (Roxb. Ex Jack) Danser) and in Silico Studies of its Isolate","authors":"Destria Roza, Gianna Fadhilah, Eka Indriani, Yuli Asih Wiliranti, T. Juwitaningsih","doi":"10.4274/tjps.galenos.2023.26243","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2023.26243","url":null,"abstract":"","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"19 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139158654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-14DOI: 10.4274/tjps.galenos.2023.68054
Sıla Gülbağ Pınar, Nevin Çelebi
{"title":"Development of Cyclosporine A Nanosuspension using Experimental Design by Response Surface Methodology: In Vitro Evaluations","authors":"Sıla Gülbağ Pınar, Nevin Çelebi","doi":"10.4274/tjps.galenos.2023.68054","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2023.68054","url":null,"abstract":"","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"24 8","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134953983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-14DOI: 10.4274/tjps.galenos.2023.37807
Özlem Çoban, Kutsal Özcan, Seçkin Engin, Buse Tatar, Mihriban Soylu
{"title":"Formulation and Evaluation of Triamcinolone Acetonide-loaded Oral Disintegrated Film with Different Polymers via Solvent Casting Method","authors":"Özlem Çoban, Kutsal Özcan, Seçkin Engin, Buse Tatar, Mihriban Soylu","doi":"10.4274/tjps.galenos.2023.37807","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2023.37807","url":null,"abstract":"","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"3 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134954403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background : A drug utilization study is a tool for determining the effectiveness of drug use. Our research aims to evaluate the drug usage patterns in Ear, Nose, and Throat (ENT) outpatient settings by incorporating established benchmarks and World Health Organization (WHO) indicators. Methods : We conducted a drug utilization evaluation (DUE) study on 800 patients from the ENT outpatient department (OPD). Initially, we gathered data on the currently prescribed medications and, through careful analysis, identified discrepancies. To rectify any irrational prescribing patterns identified among physicians, we utilized continuing educational activities like 'Dear Doctor' letters and personal consultations. We applied the WHO's core indicators, specifically prescribing and patient care indicators, and established benchmarks to encourage rational prescribing. Results : The three most common diagnoses identified were pharyngitis (51.49%), allergic rhinitis (25.11%), and Acute Suppurative Otitis Media (21.17%). Montelukast, in combination with levocetirizine (13.77%) and amoxicillin paired with clavulanic acid (8.81%), were the most frequently prescribed medications. The study revealed prescribing indicators such as an excessive number of drugs per prescription (4.2), low usage of generic names (33.0%), and suboptimal reliance on the Essential Drugs List (76.7%). Furthermore, patient care indicators demonstrated room for improvement, particularly concerning consultation times (6mins
{"title":"Prospective-Drug Utilization Evaluation Analysis in Ear, Nose, and Throat Outpatient Department: Incorporating Benchmarks and World Health Organization Indicators","authors":"Vinodkumar Mugada, Blessy Jayamon, Bindu Krishna Kosireddy, Bhagya Sri Palisetty, Apoorva Pinisetti","doi":"10.4274/tjps.galenos.2022.50246","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2022.50246","url":null,"abstract":"Background : A drug utilization study is a tool for determining the effectiveness of drug use. Our research aims to evaluate the drug usage patterns in Ear, Nose, and Throat (ENT) outpatient settings by incorporating established benchmarks and World Health Organization (WHO) indicators. Methods : We conducted a drug utilization evaluation (DUE) study on 800 patients from the ENT outpatient department (OPD). Initially, we gathered data on the currently prescribed medications and, through careful analysis, identified discrepancies. To rectify any irrational prescribing patterns identified among physicians, we utilized continuing educational activities like 'Dear Doctor' letters and personal consultations. We applied the WHO's core indicators, specifically prescribing and patient care indicators, and established benchmarks to encourage rational prescribing. Results : The three most common diagnoses identified were pharyngitis (51.49%), allergic rhinitis (25.11%), and Acute Suppurative Otitis Media (21.17%). Montelukast, in combination with levocetirizine (13.77%) and amoxicillin paired with clavulanic acid (8.81%), were the most frequently prescribed medications. The study revealed prescribing indicators such as an excessive number of drugs per prescription (4.2), low usage of generic names (33.0%), and suboptimal reliance on the Essential Drugs List (76.7%). Furthermore, patient care indicators demonstrated room for improvement, particularly concerning consultation times (6mins","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"139 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139334444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-20DOI: 10.4274/tjps.galenos.2023.23428
Ahmad Rashidi Mohamed Tahir, Voon Jia Haw, Wan Sazrina Wan Zaid, M. Nizaruddin, Arimi Fitri Mat Ludin
Objectives: To promote a holistic approach to healthcare, the University of Cyberjaya (UoC) introduced a unique elective course called sport pharmacy which incorporates components of lifestyle interventions. Customers are increasingly seeking guidance on lifestyle factors that impact their health. Pharmacists with expertise in sports and exercise can meet this demand. However, there is a lack of studies about the impact of non-pharmacological approaches on health parameters among healthcare professionals and pharmacy students in Malaysia. The objectives of this study were to compare students’ health parameters based on non-pharmacological approaches and identify the factors that motivate them to maintain their health. Materials and Methods: The study comprised of 47 Year 4 pharmacy students in pre and post practical phase (September 2018 and November 2018), followed by post-resting phase (May 2019). Data collection form in the Sport Pharmacy course was used for the data collection. Results: A little less than half of the students displayed normal Body Mass Index (BMI). After the post-resting period, there was a rise in the number of students classified as obese, as well as those with high TC and FBG. Around 23% continued to control their diet and 32% maintained their physical activity levels during the post-resting study.
{"title":"The Inclusion of Sports & Exercise Related Module into Pharmacy Curriculum: Non-pharmacological Approach on Students’ Health Parameters","authors":"Ahmad Rashidi Mohamed Tahir, Voon Jia Haw, Wan Sazrina Wan Zaid, M. Nizaruddin, Arimi Fitri Mat Ludin","doi":"10.4274/tjps.galenos.2023.23428","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2023.23428","url":null,"abstract":"Objectives: To promote a holistic approach to healthcare, the University of Cyberjaya (UoC) introduced a unique elective course called sport pharmacy which incorporates components of lifestyle interventions. Customers are increasingly seeking guidance on lifestyle factors that impact their health. Pharmacists with expertise in sports and exercise can meet this demand. However, there is a lack of studies about the impact of non-pharmacological approaches on health parameters among healthcare professionals and pharmacy students in Malaysia. The objectives of this study were to compare students’ health parameters based on non-pharmacological approaches and identify the factors that motivate them to maintain their health. Materials and Methods: The study comprised of 47 Year 4 pharmacy students in pre and post practical phase (September 2018 and November 2018), followed by post-resting phase (May 2019). Data collection form in the Sport Pharmacy course was used for the data collection. Results: A little less than half of the students displayed normal Body Mass Index (BMI). After the post-resting period, there was a rise in the number of students classified as obese, as well as those with high TC and FBG. Around 23% continued to control their diet and 32% maintained their physical activity levels during the post-resting study.","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"22 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139338665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objectives: The purpose of this study was to prepare a sustained delivery mucoadhesive-thermosensitive formulation containing poloxamer 338 (P338), poloxamer 188 (P188) and mucoadhesive agents such as chitosan (CHT) and carboxymethylcellulose (CMC) to increase the ophthalmic bioavailability of Timolol Maleate (TM). Materials and Methods: Gels were prepared by mixing different amounts of P338, P188 and mucoadhesive agents in cold isotonic water with a magnetic stirrer. The sol-gel gelation time of the gels was determined using the test tube inversion method. Viscosity measurements and analysis of mechanical properties of gel formulations were carried out. In vitro release using dialysis membrane and ex vivo permeation studies using fresh cow eyes were performed.
{"title":"Timolol Maleate In Situ Ophthalmic Mucoadhesive-Thermosensitive Gel: Development and Characterization","authors":"Sibel İLBASMIŞ TAMER, Özlem Çulcu, Eda Turan, Fahriye Figen Tirnaksiz","doi":"10.4274/tjps.galenos.2023.50723","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2023.50723","url":null,"abstract":"Objectives: The purpose of this study was to prepare a sustained delivery mucoadhesive-thermosensitive formulation containing poloxamer 338 (P338), poloxamer 188 (P188) and mucoadhesive agents such as chitosan (CHT) and carboxymethylcellulose (CMC) to increase the ophthalmic bioavailability of Timolol Maleate (TM). Materials and Methods: Gels were prepared by mixing different amounts of P338, P188 and mucoadhesive agents in cold isotonic water with a magnetic stirrer. The sol-gel gelation time of the gels was determined using the test tube inversion method. Viscosity measurements and analysis of mechanical properties of gel formulations were carried out. In vitro release using dialysis membrane and ex vivo permeation studies using fresh cow eyes were performed.","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"95 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139338884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-19DOI: 10.4274/tjps.galenos.2023.62293
O. Odekanyin, Sunday Isaiah Akanni
Objectives: In this research, Dioscorea preussii root tuber haemagglutinin was purified and its physicochemical properties determined. The antioxidative prowess and anti-haemolytic activities of the haemagglutinin were also investigated. Material and Methods: Hemagglutinating assay was used to detect the presence of lectin in the Phosphate Buffer Saline (PBS) extract of the D. preussii root tuber. The lectin was purified using ammonium sulphate fractionation and molecular sieve chromatography. Optimum pH and temperature were determined. So also, antioxidant activity was assessed using DPPH radical scavenging, metal chelating, FRAP and lipid peroxidation inhibition assays. Red blood cells subjected to oxidative damage caused by H 2 O 2 was employed to evaluate its antihemolytic ability. Results: Starch inhibited the haemagglutinating activity of the hemagglutinin. Dioscorea preussii haemagglutinin (DPH) maintained full haemagglutinating activity from 30 ºC to 60 ºC and pH 5-13. EDTA had no effect on the haemagglutinating activity of the haemagglutinin. All the denaturing agents (Guanidine-HCl, Urea and β-Mercaptoethanol
{"title":"Hemagglutinin from the Root Tuber of Dioscorea preussii Pax Exhibit Antioxidative Prowess and Haemolysis Inhibition","authors":"O. Odekanyin, Sunday Isaiah Akanni","doi":"10.4274/tjps.galenos.2023.62293","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2023.62293","url":null,"abstract":"Objectives: In this research, Dioscorea preussii root tuber haemagglutinin was purified and its physicochemical properties determined. The antioxidative prowess and anti-haemolytic activities of the haemagglutinin were also investigated. Material and Methods: Hemagglutinating assay was used to detect the presence of lectin in the Phosphate Buffer Saline (PBS) extract of the D. preussii root tuber. The lectin was purified using ammonium sulphate fractionation and molecular sieve chromatography. Optimum pH and temperature were determined. So also, antioxidant activity was assessed using DPPH radical scavenging, metal chelating, FRAP and lipid peroxidation inhibition assays. Red blood cells subjected to oxidative damage caused by H 2 O 2 was employed to evaluate its antihemolytic ability. Results: Starch inhibited the haemagglutinating activity of the hemagglutinin. Dioscorea preussii haemagglutinin (DPH) maintained full haemagglutinating activity from 30 ºC to 60 ºC and pH 5-13. EDTA had no effect on the haemagglutinating activity of the haemagglutinin. All the denaturing agents (Guanidine-HCl, Urea and β-Mercaptoethanol","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"38 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139338712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-28DOI: 10.4274/tjps.galenos.2023.10663
F. Pakdel, J. Pakdel, Ali Najmeddin, Afshin Peirovi, M. H. Nicknam, F. Dorkoosh
Introduction Developing and designing an implant with a controlled release of active protein drugs has always been a challenge. In order to optimize and control the release of insulin in this project, the drug complexing mechanism was used by dextran sulfate sodium and PHBV (Poly (3-hydroxybutyrate-co-3-hydroxyvalerate)) polymer. Methods For this purpose
{"title":"A novel controlled release implant of insulin based on Poly (3- hydroxybutyrate-co-3-hydroxyvalerate) polymer prepared by extrusion process","authors":"F. Pakdel, J. Pakdel, Ali Najmeddin, Afshin Peirovi, M. H. Nicknam, F. Dorkoosh","doi":"10.4274/tjps.galenos.2023.10663","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2023.10663","url":null,"abstract":"Introduction Developing and designing an implant with a controlled release of active protein drugs has always been a challenge. In order to optimize and control the release of insulin in this project, the drug complexing mechanism was used by dextran sulfate sodium and PHBV (Poly (3-hydroxybutyrate-co-3-hydroxyvalerate)) polymer. Methods For this purpose","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48709722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号