Knockdown of acetylcholinesterase (AChE) gene in rice yellow stem borer, Scirpophaga incertulas (Walker) through RNA interference

Q1 Agricultural and Biological Sciences Agri Gene Pub Date : 2019-03-01 DOI:10.1016/j.aggene.2019.100081

Vijaya Sudhakara Rao Kola, Renuka Pichili, Ayyagari Phani Padmakumari, Satendra Kumar Mangrauthia, Sena M. Balachandran, Maganti Sheshu Madhav

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引用次数: 7

Abstract

Yellow stem borer (YSB), Scirpophaga incertulas, (Walker) is one the most destructive pests in rice causing severe yield loss. Of the many approaches for managing insect pests, RNA interference (RNAi) is a viable option which can be employed by targeting the key insect genes that are involved in host-pest interactions. In this study, Acetylcholinesterase (AChE), a key gene of YSB was targeted for silencing through a RNAi approach. Specifically designed dsRNA from the alpha/beta hydrolase fold of YSB AChE was labeled with 5’ FAM (Fluorescent dye) and used in this study. The efficacy of dsRNA was evaluated through a systematic insect bioassay. Larval growth and mortality were observed for a period of 15 days after the treatment through the cut stem assays. The reduced larval length and weight were observed in the dsRNA treated samples which were correlated with the low gene expression compared to the untreated controls. These results suggest that AChE is a potential target for RNAi approach in insects.

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RNA干扰法敲除水稻黄二化螟乙酰胆碱酯酶（AChE）基因

黄茎螟(Scirpophaga incertulas, Walker)是水稻最具破坏性的害虫之一，造成严重的产量损失。在控制害虫的许多方法中，RNA干扰(RNAi)是一种可行的选择，它可以通过靶向参与宿主-害虫相互作用的关键昆虫基因来使用。本研究通过RNAi方法靶向YSB的关键基因乙酰胆碱酯酶(AChE)进行沉默。从YSB AChE的α / β水解酶折叠中专门设计的dsRNA用5 ' FAM(荧光染料)标记并用于本研究。通过系统的昆虫生物测定来评价dsRNA的功效。在处理后15 天，通过切茎试验观察幼虫的生长和死亡率。与未处理的对照相比，dsRNA处理样品的幼虫长度和体重减少，这与低基因表达有关。这些结果表明乙酰胆碱酯酶是RNAi方法在昆虫中的潜在靶点。

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Agri Gene Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

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期刊介绍： Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.

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