PREPARATION AND CHARACTERIZATION OF BACTERIAL CELLULOSE PRODUCED IN MODIFIED HESTERIN-SCHRAMM MEDIUM BY GLUCONACETOBACTER XYLINUS

IF 1.3 4区 农林科学 Q2 MATERIALS SCIENCE, PAPER & WOOD Cellulose Chemistry and Technology Pub Date : 2023-07-20 DOI:10.35812/cellulosechemtechnol.2023.57.46
Mohammadmahdi Alighanbari, F. Danafar, Fereshteh Bakhtiari, M. Jajarmi
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Abstract

This work investigated the suitability of using date syrup for producing bacterial cellulose (BC) by Gluconacetobacter xylinus (PTCC 1734). In the preliminary study, BC was produced in Hesterin-Schramm (HS) medium. The highest production yield was achieved after 12 days of cultivation at the initial pH of 8. Considering this result, BC was produced in modified HS media containing date syrup. In the modified HS1 (MHS1) medium, glucose was omitted, and in the modified HS2 (MHS2) medium, neither glucose nor peptone was present. Interestingly, the production yield in the modified media was about 75% higher than the production using HS medium. The amounts of BC produced using MHS1 and MHS2 media were obtained as 5.03 gL-1 and 5.17 gL-1, respectively. Structure analysis of these specimens indicated long fibrils, with diameters less than 70 nm. However, the crystallinity index of BC obtained from MHS1 media, containing peptone, was higher than that of BS produced in MHS2.
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改性hesterin-schramm培养基中木质糖醋杆菌细菌纤维素的制备及特性研究
研究了以枣糖浆为原料利用木酸糖醋杆菌(PTCC 1734)生产细菌纤维素(BC)的适宜性。在初步研究中,BC是在Hesterin-Schramm (HS)培养基中产生的。在初始pH为8的条件下,培养12天后产量最高。考虑到这一结果,在含有枣糖浆的改性HS培养基中生产BC。在改良的HS1 (MHS1)培养基中不含葡萄糖,在改良的HS2 (MHS2)培养基中不含葡萄糖和蛋白胨。有趣的是,改性培养基的产量比使用HS培养基的产量高出约75%。MHS1和MHS2培养基的BC产量分别为5.03 gL-1和5.17 gL-1。结构分析表明,这些标本的原纤维较长,直径小于70 nm。然而,从含有蛋白胨的MHS1培养基中获得的BC的结晶度指数高于在MHS2中产生的BS。
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来源期刊
Cellulose Chemistry and Technology
Cellulose Chemistry and Technology 工程技术-材料科学:纸与木材
CiteScore
2.30
自引率
23.10%
发文量
81
审稿时长
7.3 months
期刊介绍: Cellulose Chemistry and Technology covers the study and exploitation of the industrial applications of carbohydrate polymers in areas such as food, textiles, paper, wood, adhesives, pharmaceuticals, oil field applications and industrial chemistry. Topics include: • studies of structure and properties • biological and industrial development • analytical methods • chemical and microbiological modifications • interactions with other materials
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