Determination of the ovine ovarian reserve during the prenatal and neonatal periods

IF 0.5 Q4 REPRODUCTIVE BIOLOGY Asian Pacific Journal of Reproduction Pub Date : 2023-07-01 DOI:10.4103/2305-0500.380986
Isam B. Sharum
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Abstract

Objective: To determine the ovine ovarian histomorphology and follicular staging at various age periods in Awassi breed. Methods: Ovaries were collected from prenatal fetuses [gestational age (95±5) days], neonatal (day 0), and prepubertal ewe lambs (two and four months of age); each age group included six animals. Ovaries (n=12, each group) were dissected and processed for hematoxylin and eosin staining. Stained sections (n=24, each group) were imaged and utilized for histomorphology assessment, follicle measurement, and classification. Results: Prenatal ovaries were mainly enriched with primordial follicles accompanied by a lower proportion of primary follicles. In addition to primordial and primary follicles, neonatal ovaries demonstrated a proportion of centrally located multilayered and antral follicles. In comparison with neonatal ovaries, the proportion of multilayered and antral follicles was significantly higher in the ovaries of two-month-old lambs; conversely, the proportion of peripherally situated primordial follicles dramatically declined compared to that of earlier age of lamb. Although there was no statistical variation in the sizes of primordial follicles across groups, the mean diameter of the primary follicle in the prenatal ovaries was substantially smaller than in postnatal ovaries. Compared to the neonatal ovaries, the size of the multilayered and antral follicles in the prepubertal ovaries was substantially larger. Conclusions: The earliest follicular developmental stages were established prenatally whereas the advanced growth stages started in the neonatal period and greatly increased in the prepubertal period.
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产前和新生儿期绵羊卵巢储备的测定
目的:了解Awassi不同年龄段绵羊卵巢组织形态及卵泡分期。方法:从产前胎儿[胎龄(95±5)天]、新生儿(0天)和青春期前母羊羔羊(2个月龄和4个月龄)采集卵巢;每个年龄组包括6只动物。解剖卵巢(每组n=12)并进行苏木精和伊红染色。对染色切片(每组n=24)进行成像,并用于组织形态学评估、毛囊测量和分类。结果:产前卵巢主要富含原始卵泡,初级卵泡比例较低。除了原始卵泡和初级卵泡外,新生儿卵巢还显示出一定比例的位于中心的多层和窦状卵泡。与新生儿卵巢相比,两个月大的羔羊卵巢中多层卵泡和窦状卵泡的比例明显更高;相反,与早期羔羊相比,位于外围的原始卵泡的比例显著下降。尽管各组原始卵泡的大小没有统计学差异,但产前卵巢中初级卵泡的平均直径明显小于产后卵巢。与新生儿卵巢相比,青春期前卵巢中多层和窦状卵泡的大小要大得多。结论:最早的卵泡发育阶段是在产前建立的,而晚期生长阶段始于新生儿期,并在青春期前大大增加。
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来源期刊
Asian Pacific Journal of Reproduction
Asian Pacific Journal of Reproduction Veterinary-Veterinary (all)
CiteScore
1.70
自引率
0.00%
发文量
588
审稿时长
9 weeks
期刊介绍: The journal will cover technical and clinical studies related to health, ethical and social issues in field of Gynecology and Obstetrics. Articles with clinical interest and implications will be given preference.
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